Makina a Chisinthiko
Ubwino Wantchito
Takagi et al.,Buku la mbewu, 2013
●Kusanthula Kwambiri Bioinformatic:Kuthandiza kuyezeka kwa mitundu ya ma genetic, komwe kumawonetsa chisinthiko chosinthika cha mitundu, ndikuwulula ubale wodalirika wa phylogenetic pakati pa mitundu yochepetsedwa yolumikizidwa ndi chisinthiko
●Kusanthula kwachitetezo: monga kuwerengera kwa nthawi yosiyanasiyana ndi liwiro potengera kusiyanasiyana ku Nucleotide ndi Amino Ads mulingo.
●Katswiri wa ukadaulo ndi zolemba zofalitsa: BMKgene wapeza ntchito zazikuluzikulu zazambiri za anthu oposa 15, zikugwirizana ndi mitundu masauzande ambiri, ndi zina zambiri zofalitsidwa.
P: Ndi zokumana nazo zazikulu munthawi yotsogola, gulu la BMKgene limasanthula kuwunika kwathunthu ndi nthawi yotembenuka.
● Thandizo Logulitsa:Kudzipereka kwathu kumapitilira kumaliza ntchito ndi mwezi wachitatu - nthawi yogulitsa ntchito. Panthawi imeneyi, timapereka thandizo lotsatira, kuvutitsa, ndi magawo kuti athetse mafunso okhudzana ndi zotsatira zake.
Zolemba ndi zofunikira
| Mtundu wa zingwe | Olimbikitsidwa kuchuluka kwa anthu | Njira Yolowetsa | Zofunikira za Nucleotide |
| Chingwe chonse | ≥ 30 payekha, ndi ≥ 10 zochokera ku subgrouroup iliyonse
| 10x | Kuzindikira: ≥ 1 qg / μl Kuchuluka kwa≥ 30ng Kuperewera kapena kuwonongeka kapena kuipitsidwa |
| Chidutswa chapadera chowonjezera (slaf) | Kuya Kwakuya: 10x Chiwerengero cha ma tag: <400 MB: WGS akulimbikitsidwa <1GB: 100k tags 1GB > 2GB: 300K tag Ma tag a max 500k | Kukhazikika ≥ 5 ng / μl Kuchuluka kwa ≥ 80 zg Nanodropropd260 / 280 = 1.6-2.5 Agarose gel: ayi kapena kuwonongeka kochepa kapena kuipitsidwa
|
Ntchito Yogwira Ntchito
Mapangidwe a kuyesa
Kutumiza kwachitsanzo
Ntchito Yomanga Laibulale
Kusazidza
Kusanthula kwa data
Ntchito Zogulitsa Pambuyo
Ntchito imaphatikizapo kusanthula kwa kuchuluka kwa anthu (Phylogenetic Mtengo wa anthu (PCA, Triptirization Stratimition Distivition) Ntchitoyi imaphatikizidwanso kusanthula kwachipatala (mwachitsanzo, nthawi yosiyanasiyana, NAP BOW).
*Zotsatira za Demo zomwe zikuwonetsedwa pano zonse za genomes zimasindikizidwa ndi BMKGEN
Kusanthula kwa 1.evovotis komwe kumakhala kupangira mtengo wa phylogenetic, kapangidwe ka anthu ndi PCA kutengera mitundu ya majini.
Mtengo wa phylogenetic umaimira maubwenzi a msonkho ndi chisinthiko pakati pa makolo wamba.
PCA ikufuna kuona mwakulankhula pakati pa anthu wamba.
Kupanga kwa kuchuluka kwa anthu kumawonetsa kukhalapo kwa anthu osiyanasiyana pazambiri pankhani ya ma frequenc.
Chen, et. al.,Map, 2020
2.Selective Ssee
Kusankha Ssuep kumatanthauza njira yomwe tsamba labwino limasankhidwa ndi maulendo olumikizidwa ndi malo osalowerera ndale zimawonjezereka ndipo mawebusayiti osavomerezeka amachepa, ndikuchepetsa kudera.
Kuzindikira kolunjika kwa madera omwe amasankhidwa ndi kuwerengera kwa chibadwa cha kuchuluka kwa chibadwa (π, fst, a tajima a snuws onse mkati mwa zenera (100 KB) pazenera (10 KB).
Mitundu yosiyanasiyana ya nucleotide (π)
TAjima's D
Index (FST)
Wu, et. al.,Chomera cha maselo, 2018
3.Ge
Wu, et. al.,Chomera cha maselo, 2018
Mbiri Yakale
Zhang, et. al.,Zachilengedwe zachilengedwe & chisinthiko, 2021
5.Dimence nthawi
Zhang, et. al.,Zachilengedwe zachilengedwe & chisinthiko, 2021
Funsani zomwe zikugwirizana ndi BMKGE Mbiri ya BMKNEY RETERS SUSTRICS kudzera muzofalitsa zofalitsa:
Hassanyar, AK et al. .Mabungwe apadziko lonse lapansi a sayansi, 24 (7). Doi: 10.3390 / IJMS240762238.
Chai, J. et al. .Kafukufuku wa zoological, 2022, Vol. 43, Magazini 3, 469-480, 43 (3), mas. 469-480. Doi: 10,24272 / J.ESSN.2095-8137.2022.101.
Han, M. et al. (2022) 'Phylogetogrance patokha ndi mbiri ya anthu ochokera ku chilengedwe cha Elymus Sibericus L. pa Qinghai-tibetan Plateau',Madera mu Science Science, 13, p. 882601. Doi: 10.3389 / FPLS.2022.882601 / Biblex.
Wang, J. et al. .Kafukufuku wa Hurtecil, 9. Doi: 10,1093 / hr / uhac021.
