TGuide Smart Blood/Cell/Tissue RNA Kit
Nambala ya Catalog / phukusi
| Mphaka. ayi | ID | No. ya preps |
| 4995043 (mbale) | Chithunzi cha DP661-E | 96 |
| 4993551 (katiriji) | Chithunzi cha DP661-DE | 48 |
Chitsogozo chosankha Reagent cha TGuide S16
| Nuclei Acid | Mtundu Wachitsanzo | Dzina lazogulitsa | Kukula kwake (zokonzekera) Katiriji/ mbale | Mphaka no. Cartridge / mbale |
| DNA | Nyama ya Zinyama | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Chomera & Mbewu | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Nthaka/Choponda | TGuide Smart Soil /Stool DNA Kit | 48 | 4993549 |
| DNA | Gel & PCR mankhwala | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Magazi Onse | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Ma cell/Swab/Dry spots, etc | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Magazi/Maselo/Minofu | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Chomera & Mbewu | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Zamadzimadzi Zaulere Zamaselo (Seramu, ndi zina) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Chiyambi cha malonda
Yeretsani zokolola zambiri, zoyera, zamtundu wapamwamba wa RNA kuchokera ku magazi/maselo/minofu.
Palibe ma reagents oopsa monga phenol/chloroform omwe ali
RNA yopezedwa imakhala yoyera kwambiri ndipo imatha kugwiritsidwa ntchito mwachindunji pozindikira chip, kutsata kwapamwamba kwambiri ndi zoyeserera zina.
Mawonekedwe
Super yosavuta kugwiritsa ntchito
Palibe ntchito yowonjezera mapaipi.
Voliyumu ya elution ikhoza kusinthidwa ngati pakufunika.
Kuyika ndi kugwira ntchito kumafuna maphunziro ochepa. Ndi mapulogalamu omwe adakhazikitsidwa kale, tsegulani katiriji, sankhani protocol ndikuyesa kuyesa kwanu. Mapulogalamu ndi okonzeka kugwiritsidwa ntchito, komanso makonda.
Ma reagents odzaza ndi zinthu zofananira zotayidwa.
Kusamalira mankhwala kumathetsedwa kwambiri kuonetsetsa chitetezo cha ntchito.
Zotsatira za Demo
Agilent 2100 idagwiritsidwa ntchito pozindikira kukhulupirika.
Gwero lachitsanzo: mbewa za CD-1
Zitsanzo: magazi athunthu
Kukula kwachitsanzo: 200 μl
Zotsatira zoyeserera: RNA yonse yotengedwa m'magazi ndi TGuide S16 yokhala ndi reagent 4993551 ili ndi zokolola zabwino komanso chiyero chambiri, ndipo imatha kukwaniritsa zosowa zakutsika
kutsata kwapamwamba.
Gwero lachitsanzo: mbewa za CD-1
Kukweza voliyumu: 1 μl. Chiwindi chinachepetsedwa kasanu ndikudzaza.
Kuchuluka kwa gel agarose: 1%. Electrophoresis kwa mphindi 20 pa 6 V / cm;
M:TIANGEN Marker III 1-4: mtima 5-8:
chiwindi 9-12: mapapo 13-16: impso
Zitsanzo ziwiri zoyambirira za minofu iliyonse zidatengedwa ndi zida zopangira spin-column, ndipo ziwiri zomaliza zidachotsedwa zokha ndi TGuide S16.
Chotsatira choyesera: minofu ya nyama yonse ya RNA yotengedwa ndi TGuide S16 yokhala ndi reagent 4993551 imakhala ndi zokolola zambiri komanso zoyera, zomwe ndizofanana ndi RNA yotengedwa ndi protocol yotulutsa spin-column. Kuyera kumakhala kofanana, ndi OD260/OD280 mozungulira 2.0~2.1 ndi OD260/OD230> 2.0. Pomaliza, TGuide S16 itha kugwiritsidwa ntchito kuchotsa RNA yonse kuchokera kuzinthu zanyama m'malo mwa spin-column based extraction solution.
