TGuide Smart Magnetic Plant RNA Kit
Nambala ya Catalog / phukusi
| Mphaka. ayi | ID | No. ya preps |
| 4993552 (katiriji) | Chithunzi cha DP662-DE | 48 |
Chitsogozo chosankha Reagent cha TGuide S16
| Nuclei Acid | Mtundu Wachitsanzo | Dzina lazogulitsa | Kukula kwake (zokonzekera) Katiriji/ mbale | Mphaka no. Cartridge / mbale |
| DNA | Nyama ya Zinyama | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | Chomera & Mbewu | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Nthaka/Choponda | TGuide Smart Soil /Stool DNA Kit | 48 | 4993549 |
| DNA | Gel & PCR mankhwala | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Magazi Onse | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Ma cell/Swab/Dry spots, etc | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Magazi/Maselo/Minofu | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | Chomera & Mbewu | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Zamadzimadzi Zaulere Zamaselo (Seramu, ndi zina) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Chiyambi cha malonda
Yeretsani zokolola zambiri, zoyera, zamtundu wapamwamba wa RNA kuchokera ku magazi/maselo/minofu.
Palibe ma reagents oopsa monga phenol/chloroform omwe ali
RNA yopezedwa imakhala yoyera kwambiri ndipo imatha kugwiritsidwa ntchito mwachindunji pozindikira chip, kutsata kwapamwamba kwambiri ndi zoyeserera zina.
Mawonekedwe
Super yosavuta kugwiritsa ntchito
Palibe ntchito yowonjezera mapaipi.
Voliyumu ya elution ikhoza kusinthidwa ngati pakufunika.
Kuyika ndi kugwira ntchito kumafuna maphunziro ochepa. Ndi mapulogalamu omwe adakhazikitsidwa kale, tsegulani katiriji, sankhani protocol ndikuyesa kuyesa kwanu. Mapulogalamu ndi okonzeka kugwiritsidwa ntchito, komanso makonda.
Ma reagents odzaza ndi zinthu zofananira zotayidwa.
Kusamalira mankhwala kumathetsedwa kwambiri kuonetsetsa chitetezo cha ntchito.
Zotsatira za Demo
Muzu wa tirigu wonse wa RNA m'zigawo
Kukula kwachitsanzo: 100 mg
Zitsanzo pretreatment: akupera ndi madzi asafe kapena otsika kutentha minofu homogenizer mankhwala
Agarose gel osakaniza: 2% (TAE)
Kukweza voliyumu: 2 μl
Chizindikiro: Chizindikiro IV, TIANGEN
Chotsatira choyesera: kugwiritsa ntchito TGuide S16 yopezedwa ndi reagent 4993552 kuti ichotseretu RNA yonse ya mizu ya tirigu imakhala ndi zokolola zabwino komanso zoyera kwambiri. Pakuyesa uku, pafupifupi 20 μg ya nucleic acid idachotsedwa ku mizu ya tirigu ya 100 mg, ndi OD260 / OD280 kuzungulira 2.0 ~ 2.1, ndi OD260 / OD230＞2.0.
Total RNA m'zigawo zosiyanasiyana zomera zitsanzo
Kukula kwachitsanzo: 100 mg
Zitsanzo pretreatment: otsika kutentha homogenizer
Kuchuluka kwa gel agarose: 1% (TAE)
Kukweza voliyumu: 1 μl
M: Chizindikiro III, TIANGEN
1-4: mbewu za chimanga 5-8: masamba a chimanga 9-12: mizu ya kabichi 13-16: soya
Zitsanzo ziwiri zoyamba zidatengedwa ndi zida za spin-column m'zigawo ndipo zitsanzo ziwiri zomaliza zidatengedwa ndi TGuide S16.
Chotsatira choyesera: zokolola za RNA yopangidwa ndi TGuide S16 yokhala ndi reagent 4993552 ndizofanana kapena zabwinoko kuposa zomwe zidapangidwa ndi spin-column extraction. Ukhondo ndi umphumphu ndizofanana. Chifukwa chake, TGuide S16 itha kugwiritsidwa ntchito kuchotsa RNA yonse m'malo mwa spin-column based extraction solution.
