Kutalika kwathunthu mrna-nanopore
Mawonekedwe
● Kugwidwa kwa Poly-MRNA yotsatira ndi CDna Synthesis ndi Kukonzekera laibulale
● Kukhazikika kwa zolemba zonsezi
● Kusanthula kwa bioninformatic kutengera kugwirizanitsa ku generome
● Bioninformatic Persestis imaphatikizapo kungonena za mtundu chabe pa gene ndi kuwunika kwa neoform komanso kusanthula kwa LNCRNA, majini, ma genony
Ubwino Wantchito
●Kuchuluka kwa mawu pamlingo wa isoform: Kusanthula mwatsatanetsatane komanso kuwunika kolondola, kusintha komwe kungapangike pomwe kumangidwa mukamasanthula mitundu yonse
●Kuchepetsa Kufuna kwa Data:Poyerekeza ndi njira yotsatira (ngs), nanopore squening imawonetsa zofunikira za deta, kulola kuchuluka kwa majini a generation owerengeka ndi deta yaying'ono.
●Kulondola kwambiri kwa mawu owerengera: onse ku gene ndi rosoform
●Kuzindikira Zowonjezera Zowonjezera: njira zina polyadetylations, kuphatikizika kwa nes ndi lcnrna ndi majini awo
●Waluso kwambiri: Timu yathu imabweretsa chuma chambiri pantchito iliyonse, atamaliza ntchito zoposa 850 nanogore ndi kutalika kopitilira muyeso ndipo adakonza zitsanzo zopitilira 8,000.
●Thandizo la Kugulitsa Pambuyo: Kudzipereka kwathu kumapitilira kumaliza ntchito polojekiti 3 - patatha miyezi itatu kapena nthawi yogulitsa. Panthawi imeneyi, timapereka thandizo lotsatira, kuvutitsa, ndi magawo kuti athetse mafunso okhudzana ndi zotsatira zake.
Zofunikira ndi Kutumiza
| Nkhokwe ya mabuku | Njira Yolowetsa | Zambiri Zotsimikiziridwa | Kuwongolera kwapadera |
| Mwamphamvu | Audina Pe150 | 6/12 GB | Avereji yapamwamba kwambiri: Q10 |
Zofunikira:
Nucleotides:
| . (Zg / μl) | Kuchuluka (μg) | Kukhala Uliwala | Umunthu |
| ≥ 100 | ≥ 1.0 | Ode260 / 280 = 1.7-2.5 Ode260 / 230 = 0,5-2.5 Ma protein kapena opanda mapuloteni kapena kuipitsa kwa DNA kuwonetsedwa pa gel. | Kwa zomera: Rin≥.0; Kwa nyama: Rin≥7.5; 5.0 $28s / 18s≥1.0; Ochepa kapena okwera pa Baserine |
● Zomera:
Muzu, tsinde kapena petal: 450 mg
Tsamba kapena mbewu: 300 mg
Zipatso: 1.2 g
● Nyama:
Mtima kapena matumbo: 300 mg
Viscera kapena ubongo: 240 mg
Minofu: 450 mg
Mafupa, tsitsi kapena khungu: 1g
● Arthropods:
Tizilombo: 6g
Crustacea: 300 mg
● Magazi onse: 1 chubu
● Maselo: 106 malulowa
Kulimbikitsidwa Kwambiri
Chidebe: 2 ml centrifuge chubu (chinimba cha tin sichikulimbikitsidwa)
Kuyika zitsanzo: Gulu + litasintha mwachitsanzo A1, A2, A3; B1, B2, B3.
Kutumiza:
1. Ma ayezi oundana: zitsanzo zimayenera kunyamula m'matumba ndikuyikidwa m'manda oundana.
2. Machubu okwera: a RNA amatha kuwuma mu RNA kukhazikika chubu (mwachitsanzo RNASTRE®) ndikutumizidwa mu kutentha kwa firiji.
Ntchito Yogwira Ntchito
Nucleotides:
Kutumiza kwachitsanzo
Ntchito Yomanga Laibulale
Kusazidza
Kusanthula kwa data
Ntchito Zogulitsa Pambuyo
Ntchito Yogwira Ntchito
Minofu:
Mapangidwe a kuyesa
Kutumiza kwachitsanzo
Kutulutsa kwa RNA
Ntchito Yomanga Laibulale
Kusazidza
Kusanthula kwa data
Ntchito Zogulitsa Pambuyo
● Kusintha kwa data
● Kuzindikiritsa
● Kuphulika kwina
● Mawu owerengera mu gene ndi mlingo woyenera
● Kusanthula mosiyanasiyana
● Kukakamiza kumathandizira komanso zopindulitsa (mabulosi ndi dets)
Kusanthula kwina
Kusanthula kwina kwa polwodenyty (APA)
Lncrna kuneneratu
Kutchulidwa kwa fanal
Kusokoneza kwa DATS
Ma protein-protein Networks
Funsani zomwe zikugwirizana ndi BMKGene's Nanopore Amzerere-utali wa Mrna kutalika kwa zofalitsa zofalitsa.
Gong, B. Et al. . Doi: 10,1016 / J.jg.2023.01.008.
Iye, Z. et al. (2023) 'Chikumbutso Chachikulu Kwambiri cha Vendocytes cha lymphocytes cha lyndocytes chimayankha Chinsinsi cha Th1 108636. Doi: 10016 / J.Fsi.2023.102636.
Ma, y. et al. (2023) 'Kusanthula Kofananiza Kwa Pacbio ndi Ont RNA Kutulutsa kwa nemopilema nomurai venom', genomics, 115 (6), p. 110709. Doi: 10,1016 / J.YNGENO.2023.110709.
Yu, D. et al. . Doi: 10,1186 / S13287-023-03491-5 / Masago / 6.
