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Zogulitsa

Fungal Genome

Biomarker Technologies imapereka kafukufuku wa ma genome, genome yabwino komanso genome yokwanira ya fangasi kutengera cholinga cha kafukufuku wina.Kutsatizana kwa ma genome, kusonkhanitsa ndi kutanthauzira kogwira ntchito kungathe kupezedwa mwa kuphatikiza kutsatizana kwa m'badwo Wotsatira + Kutsatizana kwa mbadwo wachitatu kuti mukwaniritse msonkhano wapamwamba kwambiri wa genome.Ukadaulo wa Hi-C utha kugwiritsidwanso ntchito kuti uthandizire kuphatikiza ma genome pamlingo wa chromosome.

nsanja:PacBio Sequel II

Nanopore PromethION P48

Illumina NovaSeq Platform


Tsatanetsatane wa Utumiki

Zotsatira za Demo

Ubwino wa Utumiki

● Njira zingapo zotsatirira zomwe zilipo pazolinga zosiyanasiyana za kafukufuku

● Wodziwa zambiri pakupanga ma genome a mafangasi omwe ali ndi ma pene-complete genome opitilira 10,000.

● Gulu lothandizira ukadaulo waukatswiri lomwe limakwaniritsa zofunikira pakufufuza.

Mafotokozedwe a Utumiki

Utumiki

Njira Yotsatirira

Ubwino wotsimikizika

Nthawi yoyerekeza yozungulira

Mapu abwino a fungal

Illumina 50X+Nanopore 100X

Contig N50≥2 Mb

35 masiku ogwira ntchito

PacBio HiFi 30X

Mapu athunthu a fungal pene

Illumina 50X+Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X

Chromosome anchoring ratio>90%

45 masiku ogwira ntchito

Bioinformatics kusanthula

● Kuwongolera khalidwe la data yaiwisi

● Msonkhano wa genome

● Kusanthula chigawo cha genome

● Kufotokozera kwa jini

● Kuyerekeza kwa ma genomic

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Zitsanzo Zofunika ndi Kutumiza

Zitsanzo Zofunika:

ZaZithunzi za DNA:

Mtundu Wachitsanzo

Ndalama

Kukhazikika

Chiyero

Zithunzi za DNA

> 1.2 μg

> 20 ng/μl

OD260/280= 1.6-2.5

Za zitsanzo za minofu:

Mtundu wachitsanzo Analimbikitsa chitsanzo chithandizo Zitsanzo zosungira ndi kutumiza
Unicellular bowa Yang'anani yisiti pansi pa maikulosikopu ndikuwasonkhanitsa mu gawo lawo

Kusamutsa chikhalidwe (chokhala pafupifupi 3-4.5e9 maselo) mu 1.5 kapena 2 ml eppendorf.(Keep on ice)

Centrifuge chubu kwa mphindi 1 pa 14000 g kuti mutenge mabakiteriya ndikuchotsa mphamvu yamphamvu mosamala.

Tsekani chubu ndikuwumitsa mabakiteriya mu nayitrogeni wamadzimadzi kwa maola 1-3.Sungani chubu mu -80 ℃ furiji.

Ikani zitsanzo mu nayitrogeni wamadzimadzi kwa maola 3-4 ndikusunga mu nayitrogeni wamadzimadzi kapena -80 digiri kuti musungidwe nthawi yayitali.Kutumiza kwachitsanzo kokhala ndi ayezi wowuma ndikofunikira.
Macro bowa Minofu mu mwachangu kukula gawo tikulimbikitsidwa.

Tsukani minofu ndi madzi opanda endotoxin, ndiye 70% ethonal.

Sungani chitsanzo mu cryo-chubu.

Kuyenda kwa Ntchito Yantchito

kutumiza chitsanzo

Kupereka zitsanzo

Kukonzekera kwa Library

Kumanga laibulale

Kutsata

Kutsata

Kusanthula deta

Kusanthula deta

Pambuyo pogulitsa Services

Pambuyo pogulitsa ntchito


  • Zam'mbuyo:
  • Ena:

  • 1.Circos chithunzi pa mafangasi genomic zigawo zikuluzikulu

    3

    2.Kuyerekeza kwa genomics kusanthula: Mtengo wa Phylogenetic

    4

     

    pezani mtengo

    Lembani uthenga wanu apa ndikutumiza kwa ife

    Titumizireni uthenga wanu: