Def Novo Fungmess Metuty
BMKGene imathandizira mayankho osiyanasiyana a gengus fungus, ndikupereka chithandizo chamagulu osiyanasiyana ndikufuna kukhala ndi chiyero chokwanira. Kugwiritsa ntchito zikopa zazifupi zowerenga zokha zomwe zimapangitsa m'badwo wa genome. Kuwerenga kwakanthawi komanso kuwerengera kwa nthawi yayitali pogwiritsa ntchito nanopore kapena pacbio amaphatikizidwa kwa genganga yoyengedwa yoyenga bwino ndi mipata yayitali. Kuphatikiza apo, kuphatikiza zotsekemera za Hi-C. Ch amathandiziranso kukhoza, kumapangitsa kuti zitsimikiziro za chromosome yokwanira.
Mawonekedwe antchito
Ndi njira zitatu zomwe zingathe kusankha kutengera kuchuluka kwa mtundu wa genome.
● Njira yokonzekera matenda: Kuwerengera mwachidule ndi matenda a Novaseq Pe150.
● Njira Yabwino Kwambiri:
Kafukufuku wamtunduwu: Audina Novaseq Pe150.
Msonkhano wa Genome: Pacbio Revio (HIFI imawerengera) kapena nanomore promethoion 48.
● GLOMOSOM-Level Funome:
Kafukufuku wamtunduwu: Audina Novaseq Pe150.
Msonkhano wa Genome: Pacbio Revio (HIFI imawerengera) kapena nanomore promethoion 48.
Contrag Contchous ndi Msonkhano wa Hi-C.
Ubwino Wantchito
●Njira zingapo zopenyera: Zolinga zosiyanasiyana za kafukufuku ndi zofunikira za mtundu wa genome
●Malizitsani Bioinformaticts Graeflow:Izi zimaphatikizapo msonkhano wa genome komanso kuneneratu kwa zinthu zambiri za fukoli, Genen Rine Kutchuka, komanso kutsutsa.
●Waluso kwambiri: Ndili ndi matenda oposa 12,000 omwe asonkhana, timabweretsa zaka khumi, gulu laukadaulo waluso, zinthu zokwanira, komanso chithandizo chabwino kwambiri.
●Thandizo la Kugulitsa:Kudzipereka kwathu kumapitilira kumaliza ntchito ndi mwezi wachitatu - nthawi yogulitsa ntchito. Panthawi imeneyi, timapereka thandizo lotsatira, kuvutitsa, ndi magawo kuti athetse mafunso okhudzana ndi zotsatira zake.
Zolemba
| Kutumikila | Njira Yolowetsa | Kuwongolera kwapadera |
| Genfter genome | Audina Pe150 100X | Q30≥05% |
| Ngongole Yabwino | Kafukufuku wa Genome: Audina Pe150 50 X Msonkhano: Pacbio Hifi 30x kapena Nanopore 100x | Controg N50 ≥1MB (Pacbio Syunicallular) contrag n50MB (pott unicellular) controg n5000 ≥ (ena) |
| Chromosome-level genome | Kafukufuku wa Genome: Audina Pe150 50 X Msonkhano: Pacbio Hifi 30x kapena Nanopore 100x HI-C Msonkhano wa 100x | Contrag corchious raio> 90%
|
Ntchito Zofunikira
| Kukhazikika (zg / μl) | Kuchuluka kwathunthu (μg) | Voliyumu (μl) | Ode260 / 280 | Ode260 / 230 | |
| Pacbio | ≥20 | ≥2 | ≥20 | 1.7-2.2 | ≥1.6 |
| Nanomore | ≥40 | ≥2 | ≥20 | 1.7-2.2 | 1.0-3.0 |
| Idzima | ≥1 | ≥0.06 | ≥20 | - | - |
Mafangafu ya Unicelluular: ≥3.5x1010 malulowa
Macro bowa: ≥10 g
Ntchito Yogwira Ntchito
Kutumiza kwachitsanzo
Ntchito Yomanga Laibulale
Kusazidza
Kusanthula kwa data
Ntchito Zogulitsa Pambuyo
Mulinso kuwunika:
Kafukufuku wamtunduwu:
- Kusintha Kwatsatanetsatane
- Kuwerengera kwa Genme: Kukula, heterozygleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegleglegnegson
Msonkhano wabwino wabwino:
- Kusintha Kwatsatanetsatane
- De novoMsonkhano
- Kusanthula kwa genome chigulu: kuneneratu za ma CD ndi zinthu zingapo za mitundu
- Zogwiritsa ntchito zogwirira ntchito ndi ma database angapo (Pitani, Kegg, etc.) ndi databases (khadi, vfdb, etc.)
HI-C Msonkhano:
- Moni-Ce-Cidaida.
- Kuphatikizira kuphatikiza, kuyitanitsa ndi kuwongolera
- Kuyika kwa Msonkhano wa Hi-C: Kutengera Mapu a Refeome ndi Mapa
Kafukufuku wamtundu: K-K-C-GRA
Msonkhano wa Gen Soume: Gene Boxology (NR Database)
Msonkhano wa GenOM: Ntchito Zogwirira Ntchito (Pitani)
Funsani zomwe zikugwirizana ndi BMKGE Msonkhano wa Fungal Menome wa Bongal BioMO MOYO MOYO MWA MALO OGULITSIRA.
Hao, J. et al. (2023) 'Zophatikizika zokutira zamafuta amphaka wa bowa innotus zosavomerezeka pansi pa mitsinje',BMC Njomu, 24 (1), mas. 1-12. Doi: 10.1186 / S12864-023-09656-Z / Zithunzi / 3.
Lu, l. et al. (2023) 'Misonkho Yakulira imavumbula chisinthiko ndi tizilombo tating'onoting'ono tomwe timayang'ana tizilombo toyambitsa matenda a Pathogen Rhizoctonia crealisa',Buku la Crop, 11 (2), mas. 405-416. Doi: 10,1016 / J.CJ.2022.07.024.
Zhang, H. et al. (2023) zothandizira za mitundu inayi yofotokozera za malo oyambitsa dollar pamtundu wosiyanasiyana ',Matenda azomera, 107 (3), mas. 929-934. Doi: 10.1094 / PDIS-08-22-1921-A
Zhang, SS et al. (2023) 'Malingaliro ndi majini a katundu wa tetrapor mu bowa wa grifola Frismosa',NKHANI YA FUKA, 9 (10), p. 959. Doi: 10.3390 / Jof9100959 / S1.
