Kuyanjana kwa Chromatin kochokera ku Hi-C
Zinthu Zofunika pa Utumiki
● Kutsatana kwa Illumina NovaSeq ndi PE150.
● Ntchito imafuna zitsanzo za minofu, m'malo mwa ma nucleic acids otulutsidwa, kuti zigwirizane ndi formaldehyde ndikusunga mgwirizano wa DNA ndi mapuloteni.
● Kuyesera kwa Hi-C kumaphatikizapo kuchepetsa ndi kukonza malekezero omata ndi biotin, kutsatiridwa ndi kuzungulira malekezero osalimba omwe amabwera pamene akusunga kuyanjana. Kenako DNA imakokedwa pansi ndi mikanda ya streptavidin ndikuyeretsedwa kuti ikonzedwenso ku laibulale.
Ubwino wa Utumiki
●Kapangidwe kabwino ka Enzyme Yoletsa: kuonetsetsa kuti Hi-C ikugwira ntchito bwino kwambiri pa mitundu yosiyanasiyana yokhala ndi mawiri olumikizana olondola okwana 93%.
●Zolemba Zambiri Zaukadaulo ndi Zofalitsa:BMKGene ili ndi chidziwitso chachikulu ndi mapulojekiti opitilira 2000 a Hi-C otsatizana kuchokera ku mitundu 800 yosiyanasiyana ndi ma patent osiyanasiyana. Milandu yopitilira 100 yofalitsidwa yokhala ndi accumulative impact factor yoposa 900.
●Gulu la Bioinformatics laluso kwambiri:ndi ma patent amkati ndi ma copyright a mapulogalamu a Hi-C experiments ndi deta analysis ndi pulogalamu yodzipangira yokha deta yowonetsera.
●Thandizo Pambuyo pa Kugulitsa:Kudzipereka kwathu sikupitirira kutha kwa polojekiti ndi nthawi yautumiki wa miyezi itatu pambuyo pogulitsa. Panthawiyi, timapereka chithandizo chotsata polojekiti, thandizo la kuthetsa mavuto, ndi magawo a mafunso ndi mayankho kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira zake.
●Chidziwitso Chokwanira: timagwiritsa ntchito ma database angapo kuti tifotokoze majini ndi mitundu yosiyanasiyana yodziwika bwino ndikuchita kusanthula koyenera kwa kukulitsa, kupereka chidziwitso pa mapulojekiti angapo ofufuza.
Mafotokozedwe a Utumiki
| Laibulale | Njira Yotsatizana | Zotsatira zodziwika bwino za deta | Kusintha kwa Chizindikiro cha Hi-C |
| Laibulale ya Hi-C | Illumina PE150 | Chizunguliro cha Chromatin: 150x TAD: 50x | Chizunguliro cha Chromatin: 10Kb Kulemera: 40Kb |
Zofunikira pa Utumiki
| Mtundu wa chitsanzo | Ndalama zofunika |
| Minofu ya nyama | ≥2g |
| Magazi Athunthu | ≥2mL |
| Bowa | ≥1g |
| Minofu yaing'ono ya zomera | 1g/aliquot, ma aliquot 2-4 olimbikitsidwa |
| Maselo Olimidwa | ≥1x107 |
Kusanthula Kotsatiraku Kukuphatikizapo:
● QC ya data yosaphika;
● Mapping ndi Hi-C library QC: ma valid interaction pairs ndi Interaction Decay Exponents (IDEs);
● Kufotokozera za Kuyanjana kwa Genome: kusanthula kwa cis/trans ndi mapu olumikizirana a Hi-C;
● Kusanthula kwa kugawa kwa zipinda za A/B;
● Kuzindikira ma TAD ndi ma chromatin loops;
● Kusanthula kosiyana kwa zinthu za kapangidwe ka chromatin ya 3D pakati pa zitsanzo ndi kufotokozera kogwirizana kwa majini ogwirizana.
Kugawa kwa Cis ndi trans proportion
Mapu owunikira momwe ma chromosome amagwirira ntchito pakati pa zitsanzo
Kufalikira kwa magawo a A/B m'majini onse
Kufalikira kwa ma chromatin loops m'majini osiyanasiyana
Kuwonetsa kwa ma TAD
Fufuzani kupita patsogolo kwa kafukufuku komwe kwathandizidwa ndi ntchito za BMKGene's Hi-C sequencing kudzera mu zofalitsa zosiyanasiyana.
Meng, T. et al. (2021) 'Kusanthula kofananira kwa multi-omics kumazindikira CA2 ngati chandamale chatsopano cha chordoma',Neuro-Oncology, 23(10), masamba 1709-1722. doi: 10.1093/NEUONC/NOAB156.
Xu, L. et al. (2021) 'Kusokonekera kwa 3D ndi kukonzanso majini kumapereka chidziwitso pa matenda a NAFLD kudzera mu Hi-C, Nanopore, ndi RNA sequencing',Acta Pharmaceutica Sinica B, 11(10), tsamba 3150-3164. doi: 10.1016/J.APSB.2021.03.022.
