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Zogulitsa

Hi-C yochokera ku Chromatin Interaction

Hi-C ndi njira yopangidwa kuti ijambule kasinthidwe ka genomic pophatikiza kufufuza kuyanjana kochokera kufupi ndi kutsata kwapamwamba. Njirayi imachokera ku chromatin crosslinking ndi formaldehyde, kutsatiridwa ndi kugaya ndi kubwezeretsanso m'njira yoti tizidutswa tating'ono tating'ono tating'ono timene timagwirizanitsa timapanga katundu wa ligation. Posanja zinthu za ligation izi, ndizotheka kuphunzira gulu la 3D la genome. Hi-C imathandiza kuphunzira kugawidwa kwa magawo a genome omwe ali odzaza pang'ono (A compartments, euchromatin) ndipo amatha kukhala olembedwa bwino, ndi zigawo zomwe zimakhala zolimba kwambiri (B compartments, Heterochromatin). Hi-C itha kugwiritsidwanso ntchito kuzindikiritsa Topologically Associated Domains (TADs), zigawo za genome zomwe zimapindika ndipo zimatha kukhala ndi mawonekedwe ofanana, komanso kuzindikira malupu a chromatin, zigawo za DNA zomwe zimalumikizidwa pamodzi ndi mapuloteni komanso omwe ali. nthawi zambiri amalemeretsedwa muzinthu zowongolera. Ntchito yotsatizana ya BMKGene ya Hi-C imapatsa mphamvu ofufuza kuti afufuze kukula kwa ma genomics, ndikutsegula njira zatsopano zomvetsetsa malamulo a genome ndi zotsatira zake paumoyo ndi matenda.

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira zachiwonetsero

Zofalitsa zotsatiridwa

Mawonekedwe a Utumiki

● Kutsata pa Illumina NovaSeq ndi PE150.

● Kutumikira kumafuna zitsanzo za minofu, m'malo mwa nucleic acids, kuti zigwirizane ndi formaldehyde ndi kuteteza kugwirizana kwa DNA ndi mapuloteni.

● Kuyesera kwa Hi-C kumaphatikizapo kuletsa ndi kukonza mapeto a zomata ndi biotin, kutsatiridwa ndi kuzunguliza kwa mapeto osamveka bwino pamene mukusunga kuyanjana. DNA imakokedwa pansi ndi mikanda ya streptavidin ndikuyeretsedwa kuti ikonzekere laibulale.

Ubwino wa Utumiki

●Mulingo woyenera Kuletsa Enzyme Design: kuwonetsetsa kuti Hi-C imagwira ntchito bwino pazamoyo zosiyanasiyana zofikira 93% zovomerezeka.

●Mbiri Yambiri Yaukatswiri ndi Zofalitsa:BMKGene ili ndi chidziwitso chochuluka ndi> 2000 Hi-C yotsatirira mapulojekiti kuchokera ku mitundu 800 yosiyanasiyana ndi ma patent osiyanasiyana. Milandu yopitilira 100 yosindikizidwa yokhala ndi chiwonjezeko chopitilira 900.

●Gulu Laluso la Bioinformatics:okhala ndi ma patent a m'nyumba ndi ma copyright a mapulogalamu a zoyeserera za Hi-C ndi kusanthula deta komanso pulogalamu yodzipangira yokha yowonera.

●Thandizo Pambuyo Pakugulitsa:Kudzipereka kwathu kumapitilira kutha kwa projekiti ndi nthawi ya miyezi itatu mutagulitsa. Panthawiyi, timapereka zotsatila za polojekiti, chithandizo chazovuta, ndi magawo a Q&A kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira.

●Mawu Omveka: Timagwiritsa ntchito nkhokwe zingapo kuti tifotokoze bwino zamitundu yosiyanasiyana ndikuchita kusanthula kofananirako, kupereka zidziwitso pama projekiti angapo ofufuza.

Mafotokozedwe a Utumiki

Library

Njira Yotsatirira

Kutulutsa kovomerezeka kwa data

Hi-C Signal Resolution

Hi-C library

Chithunzi cha PE150

Chromatin loop: 150x

PA: 50x

Chromatin Lupu: 10Kb

Mtengo: 40Kb

Zofunikira pa Utumiki

Mtundu wachitsanzo	Ndalama zofunika
Nyama yanyama	≥2g
Magazi Onse	≥2mL
Bowa	≥1g
Zomera - minofu yaing'ono	1g/aliquot, 2-4 aliquotes akulimbikitsidwa
Maselo Okhazikika	≥1x10⁷

Zam'mbuyo: TGuide Smart Magnetic Plant RNA Kit

Ena: DNBSEQ malaibulale opangidwa kale

Mulinso Kusanthula Kotere:

● Data yaiwisi QC;

● Mapu ndi laibulale ya Hi-C QC: awiriawiri ogwirizana ovomerezeka ndi Interaction Decay Exponents (IDEs);

● Kufotokozera kwa Genome-wide Interaction: kusanthula kwa cis/trans ndi mapu a Hi-C;

● Kusanthula kwa gawo la A/B la kugawa;

● Kuzindikiritsa ma TAD ndi malupu a chromatin;

● Kusanthula kosiyana pazinthu zamapangidwe a chromatin a 3D pakati pa zitsanzo ndi mawu ofananirako amtundu wogwirizana nawo.

Cis ndi kugawa kwa magawo

Kutentha kwa chromosomal kuyanjana pakati pa zitsanzo

Kugawa kwamitundu yonse kwa magawo a A/B

Kugawa kwamitundu yonse kwa malupu a chromatin

Kuwona kwa ma TAD

Onani kupita patsogolo kwa kafukufuku woyendetsedwa ndi BMKGene's Hi-C sequencing services kudzera mgulu lazofalitsa.

Meng, T. et al. (2021) 'Kuwunika kofananirako kophatikizika kwama omics kumawonetsa CA2 ngati chandamale cha chordoma',Neuro-Oncology, 23(10), masamba 1709-1722. doi: 10.1093/NEUONC/NOAB156.

Xu, L. et al. (2021) 'Kusokonezeka kwa 3D ndikukonzanso ma genome kumapereka chidziwitso chapathogenesis ya NAFLD ndi kuphatikiza kwa Hi-C, Nanopore, ndi RNA',Acta Pharmaceutica Sinica B, 11(10), tsamba 3150-3164. doi: 10.1016/J.APSB.2021.03.022.