mRNA-seq (NGS) - De novo

Tsatanetsatane wa mRNA umalola kuti mbiri ya zolembedwa zonse za mRNA m'maselo azikhazikika ndipo ndiukadaulo womwe umagwiritsidwa ntchito kwambiri m'malo osiyanasiyana ofufuza. Mapaipi a BMKCloud De novo mRNA-seq adapangidwa kuti azisanthula malaibulale otsatizana a poly-A pomwe palibe genome yomwe ikupezeka. Chitolirocho chimayamba ndi kuwongolera khalidwe, kutsatiridwa ndindi novokuphatikiza zolembera ndi kusankha kwa unigene set. Kusanthula kachitidwe ka unigene kumaneneratu kutsatizana kwa ma coding (CDS) ndi kubwereza kosavuta (SSR). Pambuyo pake, kusanthula kwa mawu osiyanitsa kumapeza ma jini owonetsedwa mosiyanasiyana (DEGs) pakati pamikhalidwe yoyesedwa, kutsatiridwa ndi kafotokozedwe kantchito ndikulemeretsa kwa DEGs kuti atulutse chidziwitso chachilengedwe.