Nanopore Full-length transcriptome

Nanopore transcriptome sequencing ndi njira yamphamvu yotsatirira ma cDNA aatali, kuzindikira molondola ndi kuwerengera ma isoforms. BMKCloud Nanopore Full-Length Transcriptome Pipeline idapangidwa kuti izisanthula deta ya RNA-Seq yopangidwa papulatifomu ya Nanopore motsutsana ndi genome yodziwika bwino yodziwika bwino, yopereka kusanthula koyenera komanso kuchuluka kwa jini ndi zolemba. Pambuyo pa kuwongolera kwaubwino, zotsatizana zautali wathunthu za non-chimeric (FLNC) zimapezedwa ndipo zotsatizana zotsatizana zimajambulidwa ku ma genome kuti achotse zolembedwa zosafunikira. Kuchokera pamawu olembedwa awa, mawu amachulukitsidwa ndipo ma jini owonetsedwa mosiyanasiyana ndi zolembedwa zimazindikiridwa ndikufotokozedwa mogwira ntchito. Mapaipiwa amaphatikizanso kusanthula kwa njira zina za polyadenylation (APA), kusanthula kwamitundu ina, kusanthula kosavuta kotsatizana (SSR), kulosera za lncRNA ndi zolinga zofananira, kuneneratu zakutsatizana kwa ma coding (CDS), kusanthula kwamtundu wa jini, kusanthula kwazinthu zolembera, kulosera zamitundu yatsopano. ndi chidziwitso chogwira ntchito cha zolembedwa.