Zogulitsa

Kutsata kwamtundu wa Zomera/Zinyama Zonse

Chomera/Nyama Yonse ya Genome Sequencing Chithunzi Chowonetsedwa

Kusinthasintha kwamtundu wonse, komwe kumadziwikanso kuti WGS, kumathandizira kuwulula masinthidwe wamba komanso osowa pamtundu wonsewo kuphatikiza Single Nucleotide Polymorphism (SNP), Insertion Deletion (InDel), Structure variation (SV), ndi Copy Number Variation (CNV). ).Ma SVs amapanga gawo lalikulu la kusiyana kosiyana kusiyana ndi SNPs ndipo zimakhudza kwambiri majeremusi, zomwe zimakhudza kwambiri zamoyo.Kuwerengeranso kwanthawi yayitali kumathandizira kuzindikirika bwino kwa zidutswa zazikulu komanso kusiyanasiyana kovutirapo chifukwa kuwerenga kwautali kumapangitsa kuti zikhale zosavuta kuwoloka madera ovuta monga kubwereza tandem, madera olemera a GC/AT, ndi zigawo zosinthika kwambiri.

Platform: Illumina, PacBio, Nanopore

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Zotsatira za Demo

Nkhani Yofalitsidwa

1.Ubwino wa Utumiki

Kudziwa zambiri pakutsata ma genome pamitundu yopitilira 1000.

Milandu yopitilira 800 yosindikizidwa yokhala ndi chiwonjezeko chopitilira 4000.

Kusanthula kwatsatanetsatane kwa bioinformatics pamayitanidwe osiyanasiyana ndi kusanthula ntchito.

2. Mafotokozedwe a Utumiki

nsanja		Library	Kuzama kwa seq kovomerezeka
Ilumina		Mtengo wa 150PE	Kwa SNP, InDel imayimba ≥ 10x Kwa SV, CNY kuyimba ≥ 30x
Nanopore		8kb pa	Kwa SV, CNY kuyimba ≥ 20x
Pacbio	Mtengo CCS	15kb ku	Kwa SNP, InDel, SV, CNY kuyimba ≥ 10x

3. Zitsanzo Zofunika

nsanja		Conc.(ng/μL)	Mtengo (ng)	Chiyero		Gel agarose
				OD260/280	OD260/230	1. Chotsani gulu lalikulu popanda kapena malirekuwonongeka kumawonedwa pa gel. 2. Palibe kapena kuchepa kwa RNA kapena kuipitsidwa kwa mapuloteni
Ilumina		≥1	≥30	-	-
Nanopore		≥30	Zimatengera kutulutsa kwa data 10μg/selo	1.7-2.2	≥1.5
Pacbio	Mtengo CCS	≥50		1.7-2.2	1.8-2.5

4. Bioinformation Analysis

5. Kuyenda kwa Ntchito ya Utumiki

Kupereka zitsanzo

Kutulutsa kwa DNA

Kumanga laibulale

Kutsata

Kusanthula deta

Kutumiza kwa data

Zam'mbuyo: BMKMANU S1000 Spatial Transcriptome

Ena: Single-nucleus RNA Sequencing

1) Ziwerengero za Mapu a Genome

Table 1 Ziwerengero za zotsatira za mapu

Chithunzi 1 Kugawidwa kwa kukula kwa choyikapo ndikuwerenga kufalitsa.

2) Kusintha Kuzindikira

Chithunzi 2 Ziwerengero ndi ndemanga za SNP/INDEL/SV pakati pa Zitsanzo

Chithunzi 3 Kugawa kwa ma genome kugawa

3) Kufotokozera kwamitundu yosiyanasiyana

2019	Kulankhulana kwachilengedwe	Kuphatikizika kwa ma genome athunthu kumawonetsa chiyambi cha Brassica napus ndi genetic loci yomwe ikukhudzidwa ndikusintha kwake
2020	PNAS	Chisinthiko ndi mbiri yoweta nsomba zagolide (Carassius auratus)
2021	Plant Biotechnology Journal	Ma genome amitundu iwiri yolimidwa jute
2021	Plant Biotechnology Journal	Ma genomic signatures a masamba ndi oilseed allopolyploid Brassicajuncea ndi ma genetic loci omwe amawongolera kudzikundikira kwa glucosinolates.
2019	Chomera cha Molecular	Kuphatikizika kwa ma genome amtundu wapadziko lonse lapansi wazinthu zogwiriridwa kumawulula maziko a chibadwa cha kusiyana kwawo kwa ecotype.
2022	Kafukufuku wa Horticulture	Kusanthula kwa genome-wide association kumapereka chidziwitso cha mamolekyu pakusintha kwachilengedwe kwa kukula kwa mbewu ya chivwende
2021	Journal of Experimental Botany	Kafukufuku wa Genome-wide Association amazindikira mitundu yosiyanasiyana ya GhSAD1 yopatsa kulekerera kuzizira mu thonje.
2021	Journal of Experimental Botany	Kafukufuku wa genome-wide association ndi kufananitsa kwa transcriptome amawulula buku la QTL ndi majini osankhidwa omwe amawongolera kukula kwa petal mu rapeseed.