Kutsata kwamtundu wa Zomera/Zinyama Zonse
Mawonekedwe a Utumiki
● Kukonzekera kwa laibulale kungakhale kokhazikika kapena kopanda PCR
● Imapezeka muzitsulo zotsatizana za 4: Illumina NovaSeq, MGI T7, Nanopore Promethion P48, kapena PacBio Revio.
● Kusanthula kwa Bioinformamatic kunayang'ana pa kuzindikira kwa mitundu yosiyanasiyana: SNP, InDel, SV ndi CNV
Ubwino wa Utumiki
●Mbiri Yambiri Yaukatswiri ndi Zofalitsa: Kuchulukirachulukira pakutsata ma genome pamitundu yopitilira 1000 kwapangitsa kuti pakhale milandu yosindikizidwa yopitilira 1000 yokhala ndi mphamvu yopitilira 5000.
●Comprehensive Bioinformatics Analysis: Kuphatikizira kuyimba kwina ndi kumasulira kwa ntchito.
● Thandizo Pambuyo Pakugulitsa:Kudzipereka kwathu kumapitilira kutha kwa projekiti ndi nthawi ya miyezi itatu mutagulitsa. Panthawiyi, timapereka zotsatila za polojekiti, chithandizo chazovuta, ndi magawo a Q&A kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira.
●Mawu Omveka: Timagwiritsa ntchito nkhokwe zingapo kuti tifotokoze bwino zamitundu yosiyanasiyana ndikuchita kusanthula kofananirako, ndikupereka zidziwitso pamapulojekiti angapo ofufuza.
Mafotokozedwe a Utumiki
| Zosintha zidziwike | Njira yotsatirira | Kuzama kovomerezeka |
| SNP ndi InDel | Illumina NovaSeq PE150 kapena MGI T7 | 10x pa |
| SV ndi CNV (zolondola zochepa) | 30x pa | |
| SV ndi CNV (zolondola kwambiri) | Nanopore Prom P48 | 20x pa |
| SNPs, Indels, SV ndi CNV | PacBio Revio | 10x pa |
Zitsanzo Zofunika
| Minofu kapena yotengedwa ma nucleic acid | Illumina/MGI | Nanopore | PacBio
| ||
| Animal Viscera | 0.5-1 g | ≥ 3.5 g
| ≥ 3.5 g
| ||
| Minofu Yanyama | ≥ 5 g
| ≥ 5 g
| |||
| Mammalian Blood | 1.5 ml | ≥ 0.5 mL
| ≥ 5 ml
| ||
| Nkhuku/Nsomba Magazi | ≥ 0.1 mL
| ≥ 0.5 mL
| |||
| Chomera - Tsamba Latsopano | 1-2 g pa | ≥ 2 g
| ≥ 5 g
| ||
| Maselo Okhazikika |
| ≥ 1x107
| ≥ 1x108
| ||
| Tizilombo tofewa/Payekha | 0.5-1 g | ≥ 1 g
| ≥ 3 g
| ||
| DNA yotengedwa
| Kukhazikika: ≥ 1 ng/µL Kuchuluka: ≥ 30 ng Zochepa kapena zopanda kutsitsa kapena kuipitsidwa
| Kukhazikika Ndalama
OD260/280
OD260/230
Zochepa kapena zopanda kutsitsa kapena kuipitsidwa
| ≥ 40 ng/µL 4 µg/flow cell/chitsanzo
1.7-2.2
≥1.5 | Kukhazikika Ndalama
OD260/280
OD260/230
Zochepa kapena zopanda kutsitsa kapena kuipitsidwa | ≥ 50 ng/µL 10 µg/flow cell/chitsanzo
1.7-2.2
1.8-2.5 |
| Kukonzekera kwa Library Yopanda PCR: Kukhazikika≥ 40 ng/µL Kuchuluka≥ 500 ng | |||||
Kuyenda kwa Ntchito Yantchito
Kupereka zitsanzo
Kutulutsa kwa DNA
Kumanga laibulale
Kutsata
Kusanthula deta
Kutumiza kwa data
Mulinso zowunikira izi:
- Yaiwisi Data Quality Control
- Ziwerengero za kuyanjanitsa ku genome
- Chizindikiritso chosiyana: SNP, InDel, SV ndi CNV
- Kufotokozera kwamitundu yosiyanasiyana
Ziwerengero zamalumikizidwe ku ma genome - kutsatizana kugawa kwakuya
Kuyimba kwa SNP pakati pa zitsanzo zingapo
Chizindikiritso cha InDel - ziwerengero za kutalika kwa InDel m'chigawo cha CDS ndi dera lonse la genome
Kugawa kosinthika kudutsa ma genome - Circos plot
Kufotokozera kogwira ntchito kwa majini okhala ndi mitundu yodziwika - Gene Ontology
Chai, Q. et al. (2023) 'A glutathione S-transferase GhTT19 imatsimikizira mtundu wa petal pigmentation kudzera pakuwongolera kuchuluka kwa anthocyanin mu thonje', Plant Biotechnology Journal, 21 (2), p. 433. doi: 10.1111/PBI.13965.
Cheng, H. et al. (2023) 'Chromosome-level wild Hevea brasiliensis genome imapereka zida zatsopano zoswana mothandizidwa ndi ma genomic ndi malo ofunikira kuti akweze zokolola za rabara', Plant Biotechnology Journal, 21(5), pp. 1058–1072. doi: 10.1111/PBI.14018.
Li, A. et al. (2021) 'Genome of the estuarine oyster imapereka zidziwitso pakusintha kwanyengo komanso pulasitiki yosinthika', Communications Biology 2021 4:1, 4(1), pp. 1-12. doi: 10.1038/s42003-021-02823-6.
Zeng, T. et al. (2022) 'Kuwunika kwa kusintha kwa ma genome ndi methylation mu nkhuku zachi China pakapita nthawi kumapereka chidziwitso pakusunga mitundu', Communications Biology, 5(1), pp. 1-12. doi: 10.1038/s42003-022-03907-7.
