-
PacBio-Yonse Yotalika 16S/18S/ITS Amplicon Sequencing
Pulatifomu ya Amplicon (16S/18S/ITS) yapangidwa ndi zaka zambiri zogwira ntchito mu kusanthula kwa mapulojekiti osiyanasiyana a tizilombo toyambitsa matenda, omwe ali ndi kusanthula koyambira kokhazikika komanso kusanthula kwapadera: kusanthula koyambira kumaphimba zomwe zili mkati mwa kafukufuku wamakono wa tizilombo toyambitsa matenda, zomwe zili mkati mwa kusanthulako ndi zambiri komanso zodzaza, ndipo zotsatira za kusanthula zimaperekedwa mu mawonekedwe a malipoti a projekiti; Zomwe zili mkati mwa kusanthula kwapadera ndizosiyanasiyana. Zitsanzo zitha kusankhidwa ndipo magawo amatha kukhazikitsidwa mosinthasintha malinga ndi lipoti loyambira la kusanthula ndi cholinga chofufuza, kuti akwaniritse zofunikira zaumwini. Makina ogwiritsira ntchito a Windows, osavuta komanso achangu.
-
PacBio-Transcriptome Yonse Yautali (Yosatchulidwa)
Pogwiritsa ntchito deta ya Pacific Biosciences (PacBio) Isoform sequencing ngati yolowera, pulogalamuyi imatha kuzindikira ma transcript sequences a utali wonse (popanda kusonkhana). Mwa kupanga ma transcripts a utali wonse motsutsana ndi genome yofotokozera, ma transcripts amatha kukonzedwa ndi majini odziwika, ma transcripts, madera olemba ma code, ndi zina zotero. Pankhaniyi, kuzindikira kolondola kwa kapangidwe ka mRNA, monga alternative splicing, ndi zina zotero, kungapezeke. Kusanthula kogwirizana ndi deta ya NGS transcriptome sequencing kumathandiza kufotokoza bwino komanso kuwerengera kolondola kwa ma expression pa level ya transcript, zomwe zimapindulitsa kwambiri ma differential expression ndi functional analysis.
-
Zida Zothandizira
BMKCloud ndi nsanja yotsogola ya bioinformatic yomwe imapereka yankho limodzi la mapulogalamu a genomic, lomwe ofufuza ambiri amalikhulupirira m'magawo osiyanasiyana kuphatikiza zamankhwala, zaulimi, zachilengedwe, ndi zina zotero. BMKCloud yadzipereka kupereka ntchito zophatikizana, zodalirika komanso zogwira mtima kuphatikiza nsanja ndi zida zowunikira za bioinformatic, zida zamakompyuta, database ya anthu onse, maphunziro apaintaneti a bioinformatic, ndi zina zotero. BMKCloud ili ndi zida zosiyanasiyana zomwe zimagwiritsidwa ntchito pafupipafupi za bioinformatic kuphatikiza kufotokozera majini, zida za majini osinthika, ncRNA, kuwongolera khalidwe la deta, kusonkhana, kulinganiza, kuchotsa deta, kusintha kwa majini, ziwerengero, wopanga zifaniziro, kusanthula kwa magawo, ndi zina zotero.
-
RNA Yaing'ono
Ma RNA ang'onoang'ono ndi mtundu wa RNA yochepa yopanda ma code yokhala ndi kutalika kwapakati pa 18-30 nt, kuphatikiza miRNA, siRNA ndi piRNA. Ma RNA ang'onoang'ono awa anenedwa kuti amagwira ntchito m'njira zosiyanasiyana zamoyo monga kuwonongeka kwa mRNA, kuletsa kumasulira, kupangika kwa heterochromatin, ndi zina zotero. Kusanthula kwa SmallRNA sequencing kwagwiritsidwa ntchito kwambiri mu maphunziro okhudza chitukuko cha nyama/zomera, matenda, kachilombo, ndi zina zotero. Pulatifomu yosanthula ya Small RNA sequencing imakhala ndi kusanthula kokhazikika komanso kuwunika deta kwapamwamba. Pamaziko a deta ya RNA-seq, kusanthula kokhazikika kumatha kukwaniritsa kuzindikira ndi kulosera kwa miRNA, kulosera kwa majini a miRNA, kusanthula kwa mawu ndi kufotokozera. Kusanthula kwapamwamba kumathandiza kusaka ndi kuchotsa miRNA mwamakonda, kupanga diagram ya Venn, miRNA ndi kumanga netiweki ya majini a target.
-
NGS-WGS (Illumina/BGI)
NGS-WGS ndi nsanja yonse yowunikiranso ma genome, yomwe idapangidwa potengera luso lambiri mu Biomarker Technologies. Nsanja yosavuta kugwiritsa ntchito iyi imalola kutumiza mwachangu njira yowunikira yophatikizika pongokhazikitsa magawo ochepa oyambira, omwe amagwirizana ndi deta yowunikira ya DNA yopangidwa kuchokera ku nsanja ya Illumina ndi nsanja yowunikira ya BGI. Nsanja iyi imagwiritsidwa ntchito pa seva ya computing yogwira ntchito bwino kwambiri, yomwe imathandizira kusanthula deta kogwira mtima kwambiri munthawi yochepa kwambiri. Kufufuza deta payekha kumapezeka potengera kusanthula kokhazikika, kuphatikiza kusanthula kwa majini osinthika, kapangidwe ka PCR primer, ndi zina zotero.
-
mRNA (Chidziwitso)
Transcriptome ndi mgwirizano pakati pa chidziwitso cha majini a genomic ndi proteome ya ntchito ya zamoyo. Kulamulira mulingo wa transcriptome ndiye njira yofunika kwambiri komanso yophunziridwa kwambiri ya zamoyo. Kutsata transcriptome kumatha kutsata transcriptome nthawi iliyonse kapena pansi pa vuto lililonse, ndi resolution yolondola ku nucleotide imodzi. Imatha kuwonetsa mosinthasintha mulingo wa kulembedwa kwa majini, kuzindikira nthawi yomweyo ndikuwerengera zolembedwa zosowa komanso zachizolowezi, ndikupereka chidziwitso cha kapangidwe ka zolembedwa zinazake.
Pakadali pano, ukadaulo wowerengera ma transcriptome wagwiritsidwa ntchito kwambiri mu agronomy, mankhwala ndi magawo ena ofufuza, kuphatikizapo malamulo okhudza chitukuko cha zinyama ndi zomera, kusintha kwa chilengedwe, kuyanjana kwa chitetezo cha mthupi, malo a majini, kusintha kwa majini a mitundu ndi kuzindikira matenda a chotupa ndi majini.
-
Metagenomics (NGS)
Pulatifomu yowunikirayi idapangidwa kuti iwonetse deta ya metagenomic pogwiritsa ntchito zaka zambiri. Ili ndi njira yolumikizirana yophatikizana yokhala ndi kusanthula kosiyanasiyana kwa metagenomics komwe kumafunikira kwambiri kuphatikiza kukonza deta, maphunziro amitundu, maphunziro a jini, kuyika metagenome, ndi zina zotero. Kuphatikiza apo, zida zosungira deta zomwe zasinthidwa zimapezeka pogwiritsa ntchito njira yowunikira yokhazikika, kuphatikiza kufufuza kwa majini ndi mitundu, kukhazikitsa magawo, kupanga ziwerengero zomwe zasinthidwa, ndi zina zotero.
-
LncRNA
Ma RNA aatali osalemba ma code (lncRNA) ndi mtundu wa zolembedwa zomwe zimakhala ndi kutalika kopitilira 200 nt, zomwe sizingathe kulemba ma protein. Umboni wochuluka ukusonyeza kuti ma lncRNA ambiri amatha kugwira ntchito. Ukadaulo wotsatira ma algorithms apamwamba komanso zida zowunikira za bioinformatics zimatipatsa mphamvu yovumbulutsa ma lncRNA sequences ndi kufotokozera zambiri moyenera ndikutitsogolera kupeza ma lncRNA okhala ndi ntchito zofunika kwambiri zowongolera. BMKCloud imanyadira kupatsa makasitomala athu nsanja yosanthula ma lncRNA sequencing kuti akwaniritse kusanthula kwa lncRNA mwachangu, kodalirika komanso kosinthasintha.
-
GWAS
Kafukufuku wa mgwirizano wa genome-wide association (GWAS) cholinga chake ndi kuzindikira mitundu yosiyanasiyana ya majini (genotype) yomwe imagwirizana ndi makhalidwe enaake (phenotype). Kafukufuku wa GWA amafufuza zizindikiro za majini zomwe zimadutsa genome yonse ya anthu ambiri ndipo amaneneratu kugwirizana kwa genotype-phenotype pogwiritsa ntchito kusanthula kwa ziwerengero pamlingo wa anthu. Kutsatana kwa genome yonse kumatha kupeza mitundu yonse ya majini. Kuphatikiza ndi deta ya phenotypic, GWAS ikhoza kukonzedwa kuti izindikire SNPs zokhudzana ndi phenotype, QTLs ndi majini ovomerezeka, zomwe zimathandizira kwambiri kubereka nyama/zomera zamakono. SLAF ndi njira yosavuta yotsatirira genome, yomwe imapeza zizindikiro zogawidwa za genome, SNP. Ma SNP awa, monga zizindikiro za majini a molecular, amatha kukonzedwa kuti agwirizane ndi makhalidwe omwe akufuna. Ndi njira yotsika mtengo yodziwira mitundu yovuta yogwirizana ndi majini.
-
Nanopore Full-length transcripttomics
Ma isoform ovuta komanso osiyanasiyana m'zamoyo ndi njira zofunika kwambiri za majini powongolera kufalikira kwa majini ndi kusiyanasiyana kwa mapuloteni. Kuzindikira molondola kapangidwe ka zolembedwa ndiye maziko ophunzirira mozama za machitidwe olamulira kufalikira kwa majini. Pulatifomu yotsatizana ya nanopore yabweretsa bwino kafukufuku wa transcriptomic ku isoform-level. Pulatifomu yowunikirayi idapangidwa kuti iwunike deta ya RNA-Seq yopangidwa pa nsanja ya Nanopore pogwiritsa ntchito genome yowunikira, yomwe imakwaniritsa kusanthula kwamtundu ndi kuchuluka kwa majini komanso kuchuluka kwa zolembedwa.
-
circ-RNA
RNA yozungulira (circRNA) ndi mtundu wa RNA yosalemba ma code, yomwe posachedwapa yapezeka kuti imagwira ntchito yofunika kwambiri pa maukonde olamulira omwe amakhudzidwa ndi kukula, kukana chilengedwe, ndi zina zotero. Yosiyana ndi mamolekyu a RNA olunjika, mwachitsanzo mRNA, lncRNA, malekezero a 3′ ndi 5′ a circRNA amalumikizidwa pamodzi kuti apange kapangidwe kozungulira, komwe kamawateteza ku kugaya kwa exonuclease ndipo ndi okhazikika kuposa ma RNA ambiri olunjika. CircRNA yapezeka kuti ili ndi ntchito zosiyanasiyana pakulamulira kafotokozedwe ka majini. CircRNA imatha kugwira ntchito ngati ceRNA, yomwe imalumikiza miRNA mopikisana, yotchedwa siponji ya miRNA. Pulogalamu yowunikira ya CircRNA sequencing imapatsa mphamvu kapangidwe ka circRNA ndi kusanthula kafotokozedwe, kulosera cholinga ndi kusanthula pamodzi ndi mitundu ina ya mamolekyu a RNA.
-
BSA
Pulatifomu ya Bulked Segregant Analysis imakhala ndi kusanthula kwapadera kwa sitepe imodzi komanso kusanthula kwapamwamba ndi ma parameter okonzedwa mwamakonda. BSA ndi njira yomwe imagwiritsidwa ntchito kuzindikira mwachangu zizindikiro za majini zogwirizana ndi phenotype. Njira yayikulu yogwirira ntchito ya BSA ili ndi: 1. kusankha magulu awiri a anthu omwe ali ndi mitundu yosiyana kwambiri; 2. kuphatikiza DNA, RNA kapena SLAF-seq (Yopangidwa ndi Biomarker) ya anthu onse kuti apange DNA yambiri; 3. kuzindikira magawo osiyanasiyana motsutsana ndi genome yodziwika kapena pakati, 4. kulosera madera olumikizidwa ndi ED ndi SNP-index algorithm; 5. Kusanthula kwantchito ndi kukulitsa majini m'madera omwe ali ndi mitundu, ndi zina zotero. Kufufuza kwapamwamba kwambiri mu data kuphatikiza kuyesa kwa majini ndi kapangidwe ka primer kuliponso.
-
Amplicon (16S/18S/ITS)
Pulatifomu ya Amplicon (16S/18S/ITS) yapangidwa ndi zaka zambiri zogwira ntchito mu kusanthula kwa mapulojekiti osiyanasiyana a tizilombo toyambitsa matenda, omwe ali ndi kusanthula koyambira kokhazikika komanso kusanthula kwapadera: kusanthula koyambira kumaphimba zomwe zili mkati mwa kafukufuku wamakono wa tizilombo toyambitsa matenda, zomwe zili mkati mwa kusanthulako ndi zambiri komanso zodzaza, ndipo zotsatira za kusanthula zimaperekedwa mu mawonekedwe a malipoti a projekiti; Zomwe zili mkati mwa kusanthula kwapadera ndizosiyanasiyana. Zitsanzo zitha kusankhidwa ndipo magawo amatha kukhazikitsidwa mosinthasintha malinga ndi lipoti loyambira la kusanthula ndi cholinga chofufuza, kuti akwaniritse zofunikira zaumwini. Makina ogwiritsira ntchito a Windows, osavuta komanso achangu.
