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DNBSEQ malaibulale opangidwa kale
DNBSEQ, yopangidwa ndi MGI, ndiukadaulo waukadaulo wa NGS womwe wakwanitsa kutsitsa mtengo wotsatizana ndikuwonjezera zotuluka. Kukonzekera kwa malaibulale a DNBSEQ kumaphatikizapo kugawikana kwa DNA, kukonzekera kwa ssDNA, ndikukweza mozungulira kuti mupeze ma nanoballs a DNA (DNB). Izi zimayikidwa pamalo olimba ndikutsatiridwa ndi combinatorial Probe-Anchor Synthesis (cPAS). Ukadaulo wa DNBSEQ umaphatikiza zabwino zokhala ndi cholakwika chokulitsa chocheperako ndikugwiritsa ntchito zolakwika za kachulukidwe kakang'ono ndi ma nanoballs, zomwe zimapangitsa kutsatana ndi kutulutsa kwakukulu komanso kulondola.
Ntchito yathu yotsatirira laibulale yopangidwa kale imathandizira makasitomala kukonzekera malaibulale otsatizana a Illumina kuchokera kumagwero osiyanasiyana (mRNA, genome yonse, amplicon, malaibulale 10x, pakati pa ena), omwe amasinthidwa kukhala malaibulale a MGI m'ma laboratories athu kuti atsatidwe mu DNBSEQ-T7, kuchuluka kwa data pamitengo yotsika.
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malaibulale opangidwa kale ndi Illumina
Tekinoloje yotsatizana ya Illumina, yozikidwa pa Sequencing by Synthesis (SBS), ndiukadaulo wapadziko lonse wa NGS, womwe umapangitsa kuti pakhale 90% yazotsatizana zapadziko lonse lapansi. Mfundo ya SBS imaphatikizapo kujambula zoziziritsa zotchedwa fulorosenti zotchedwa reversible terminators pamene dNTP iliyonse ikuwonjezedwa, ndipo kenako imadulidwa kuti ilole kuphatikizidwa kwa maziko otsatirawa. Ndi ma dNTP onse anayi osinthika omangika otsekera omwe amapezeka mumayendedwe aliwonse, mpikisano wachilengedwe umachepetsa kukondera. Ukadaulo wosunthikawu umathandizira malaibulale owerengeka okha komanso ophatikizidwa awiriawiri, popereka ma genomic applications. Illumina sequencing amatha kuchita bwino kwambiri komanso kulondola kwake kumayiyika ngati mwala wapangodya pa kafukufuku wa genomics, kupatsa mphamvu asayansi kuti adziwe zovuta za ma genomes mwatsatanetsatane komanso mosayerekezeka.
Ntchito yathu yotsatirira laibulale yopangidwa kale imathandizira makasitomala kukonzekera malaibulale otsatizana kuchokera kumagwero osiyanasiyana (mRNA, genome yonse, amplicon, malaibulale 10x, pakati pa ena). Pambuyo pake, malaibulalewa amatha kutumizidwa kumalo athu otsatizana kuti aziwongolera komanso kusanja pamapulatifomu a Illumina.
