Zogulitsa

Kutsata kwa Prokaryotic RNA

Sequencing ya Prokaryotic RNA imagwiritsa ntchito kutsata kwa m'badwo wotsatira (NGS) kuwulula kupezeka ndi kuchuluka kwa RNA panthawi inayake, posanthula kusintha kwa ma transcriptome.Tsatanetsatane wa kampani yathu ya prokaryotic RNA, makamaka imayang'ana ma prokaryotes okhala ndi ma genome, kukupatsirani mbiri ya transcriptome, kusanthula kapangidwe ka majini, ndi zina zambiri. Zagwiritsidwa ntchito kwambiri pakufufuza koyambira kwa sayansi, kafukufuku wamankhwala ndi chitukuko, ndi zina zambiri.

Pulatifomu: Illumina NovaSeq Platform

Lumikizanani nafe

Tsatanetsatane wa Utumiki

Mafotokozedwe a Utumiki

Platform: nsanja ya Illumina NovaSeq, PE150

Mtundu wa laibulale: 350bp ikani laibulale ya cDNA (malingana ndi kugawa kwakukulu)

Njira yotsatirira: Paired-End 150 bp

Limbikitsani kuchuluka kwa data: 2 Gb yaiwisi data/chitsanzo

Bioinformatics

(1) Kutsata kuwongolera kwamtundu wa data: Kugawa kwa Nucleotide, kugawa kwapamwamba kwa maziko, kuwunika kwa chiŵerengero cha rRNA;

(2) Kusanthula kwa kutsatizana kotsatizana: Ziwerengero za zotsatira zotsatizana, Machulukitsidwe a kutsatizana, Kufotokozera za kutsatizana;

(3) Zofotokozera za genome (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Kusanthula kwachidziwitso: Kugawa kufotokozera (Ndi zitsanzo ziwiri kapena zingapo), Kusanthula kwa mawu pakati pa zitsanzo (Venn kusanthula, kusanthula kwa Correlation, kusanthula kwa PCA);

(5) Kusanthula kwamafotokozedwe kosiyana (Ndi zitsanzo ziwiri kapena zingapo);

(6) Kusanthula kwa ma gene: Kusanthula kwa Venn, Kusanthula kwa Cluster, Kusanthula kwa ntchito (COG, GO, KEGG), Kusanthula kwachidziwitso chantchito (GO, KEGG), Ntchito yopititsa patsogolo chordal graph, kusanthula kwa Ipath;

(7) sRNA kusanthula: sRNA kulosera, sRNA annotation (Blast, sRNAMAp, sRNATarBase, SIPHT, Rfam), sRNA secondary structure prediction, sRNA target gene kulosera;

(8) Kusanthula kapangidwe kazolemba: Kusanthula kwa Operon, Kuneneratu koyambira ndi kutsiriza kwa masamba, kutanthauzira kwa UTR ndi kusanthula, Kuneneratu kwatsatizana kwa Otsatsa, kusanthula kwa SNP/InDel (SNP/InDel annotation, SNP/InDel distribution region, SNP statistics).