Kuchepetsa choyimira cha Bisuulfite
Kuchepetsa choyimira cha Bisuulfite (RRBS) yatuluka ngati njira yotsika mtengo komanso yothandizanso kutsata kwakukulu kwa mitundu yonse (WGB) mu kafukufuku wa DNATYALA. Ngakhale ma wgbs amapereka chidziwitso chokwanira popenda mtundu wonse wa maziko osafunikira, mtengo wake wokwera umatha kukhala chinthu chofooka. Ma RRS amasinthana kwambiri vutoli powunika mosamala gawo loimira. Njirayi imadalira zopindulitsa za madera olemera a CPG ndi VII Cleavage idatsatiridwa ndi kukula kwa 200-500 / 600 ma ragts. Zotsatira zake, zigawo zokhazokha zomwe zimapangitsa kuti zilumba za CPG zitsatidwe, pomwe zomwe zili ndi Islands za ku CPG sizimasungidwa chifukwa cha kusanthula. Njirayi, yophatikizidwa ndi zingwe za bisuufite, zimalola kuti zikhale ndi methylation wambiri, ndi njira yosinthira, pe150, imayang'ana kwambiri pamapeto a malekezero a malekezero a methyty ruction. A RRB ndi chida chamtengo wapatali chomwe chimathandizira kafukufuku wa DNATYALAMA NDIPONSO KUDZIPEREKA KWA ZINSINSI za Epigenic.
Mawonekedwe antchito
● Kufunanso mbiri yakale.
● Mwanawa DNA amagwiritsidwa ntchito kuwunika njira ya bisulfite yogwira ntchito.
● Chigoba chigalasi chimakhalanso chowunikiranso.
● Chimbudzi pang'onopang'ono a enzy.
● Sequenina Novaseq.
Ubwino Wantchito
●Zothandiza komanso zothandiza ku WGBS: Kupangitsa kusanthula kuchitika pamtengo wotsika komanso zomwe zimapangitsa kuti zichitike.
●Malizitsani nsanja:Muzipereka ntchito yabwino kwambiri kuchokera ku zitsanzo, zomanga la laibulale, komanso zopindika ku kusanthula bioinmatis.
●Waluso kwambiri: Ndi ma projekiti a RRBS Spequen adamaliza bwino kwambiri mitundu yosiyanasiyana ya zinthu zosiyanasiyana, ndipo gulu laukadaulo waluso kwambiri, lofananira kwathunthu, komanso chithandizo chabwino kwambiri.
Zolemba
| Nkhokwe ya mabuku | Njira Yolowetsa | Zochita zotsimikizika deta | Kuwongolera kwapadera |
| MSPI yoganda ndi laibulale ya Bisuulfite | Audina Pe150 | 8 GB | Q30 ≥ 85% Kutembenuka kwa Bisuulfite> 99% MsPi kudula bwino ntchito> 95% |
Zofunikira Zitsanzo
| Kukhazikika (zg / μl) | Kuchuluka kwathunthu (μg) |
| |
| Gen DNA | ≥ 30 | ≥ 1 | Kuwonongeka kochepa kapena kuipitsidwa |
Ntchito Yogwira Ntchito
Kutumiza kwachitsanzo
Ntchito Yomanga Laibulale
Kusazidza
Kusanthula kwa data
Ntchito Zogulitsa Pambuyo
Mulinso kuwunika:
● Kuletsa kwapadera;
● Mapu okhala ndi matchulidwe;
● Kuzindikira 5mc methylat ndi chizindikiritso cha Motif;
● Kusanthula za kufalitsa kwa methylation ndi fanizo lachitsanzo;
● Kusanthula zigawo za methyty (madrs);
● Kuchulukitsa kwa zifaniziro zolumikizidwa ndi ma DMR.
Kuwongolera kwapadera
Kuwongolera kwapadera: Kutembenuka kwa Bisulfite (m'thupi la methylation))
Mapu a Methylation: 5mc methylation genome-kufalitsa kwakukulu
Kufanizira zitsanzo: Kusanthula kwakukulu
Madera osiyanasiyana a methyted (ma dorrs): kutentha
Funsani ntchito yofufuza yomwe ilipo ndi mitundu yonse ya BMKEEN ya BMISFFFIME ya BIsulfite kudzera mu zofalitsa zofalitsa.
Li, z. et al. .PNAS Nexus, 1 (4). Doi: 10,1093 / Pnasnexus / PGAC179.
Tian, H. et al. (2023) 'Genome-DNA Methylation Kusanthula kwa thupi mu Chinese Monozygotic SINES',European nyuzipepala yazachipatala, 53 (11), p. E14055. Doi: 10,11111 / ECI.14055.
Wu, y. et al. (2022) 'DNA Methylation ndi m'chiuno-m'chiuno: Phunziro la Assigenome-Laurth mu China Monozygotic Mapasa',Journal of Endocrininological, 45 (12), mas. 2365-2376. Doi: 10.1007 / S40618-0238785.
