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Zogulitsa

Small RNA sequencing-Illumina

Mamolekyu ang'onoang'ono a RNA (sRNA), omwe nthawi zambiri amakhala pansi pa 200 nucleotide m'litali, amaphatikizapo ma microRNAs (miRNAs), ma RNAs (siRNAs), ndi ma RNA (piRNAs) omwe amasokoneza piwi.Mwa awa, ma miRNA, ozungulira ma nucleotide 20-24 aatali, ndiwodziwika kwambiri chifukwa cha maudindo awo ofunikira pamachitidwe osiyanasiyana am'manja.Pokhala ndi mawonekedwe okhudzana ndi minofu komanso siteji, ma miRNA amawonetsa kusamala kwambiri pamitundu yosiyanasiyana.

Platform: Illumina NovaSeq


Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira za Demo

Zofalitsa Zowonetsedwa

Mawonekedwe

● Kusankha kukula kwa RNA isanayambe kukonzekera laibulale

● Kusanthula kwa Bioinformamatic kunakhazikika pa kulosera kwa miRNA ndi zolinga zawo

Ubwino wa Utumiki

Kusanthula kwathunthu kwa bioinformatics:kupangitsa kuzindikirika kwa ma miRNA odziwika komanso atsopano, kuzindikiritsa zolinga za miRNAs ndi mawu ofananirako ogwira ntchito ndikulemeretsa ndi ma database angapo (KEGG, GO)

Kuwongolera Kwabwino Kwambiri: Timagwiritsa ntchito mfundo zowongolera pamagawo onse, kuyambira pakukonza zachitsanzo ndi laibulale mpaka kutsatizana ndi bioinformatics.Kuyang'anitsitsa mosamalitsa kumeneku kumatsimikizira kuperekedwa kwa zotsatira zabwino nthawi zonse.

Thandizo la Post-Sales: Kudzipereka kwathu kumapitilira kutha kwa projekiti ndi nthawi ya miyezi itatu mutagulitsa.Panthawiyi, timapereka zotsatila za polojekiti, chithandizo chazovuta, ndi magawo a Q&A kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira.

Katswiri Wazambiri: Ndi mbiri yakutseka bwino mapulojekiti angapo a sRNA okhala ndi mitundu yopitilira 100 m'magawo osiyanasiyana ofufuza, gulu lathu limabweretsa zokumana nazo zambiri pantchito iliyonse.

Zitsanzo Zofunika ndi Kutumiza

Library

nsanja

Deta yovomerezeka

Zithunzi za QC

Kukula kwasankhidwa

Illumina SE50

10M-20M imawerengedwa

Q30≥85%

Zitsanzo Zofunika:

Nucleotides:

Conc.(ng/μl)

Mtengo (μg)

Chiyero

Umphumphu

≥80

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Zochepa kapena zopanda mapuloteni kapena DNA kuipitsidwa komwe kumawonetsedwa pa gel.

RIN≥6.5;

5.0≥28S/18S≥1.0;

kukwera kochepa kapena kopanda koyambira

● Zomera:

Muzu, tsinde kapena petal: 450 mg

Tsamba kapena Mbewu: 300 mg

Chipatso: 1.2 g

● Chinyama:

Mtima kapena matumbo: 450 mg

Viscera kapena Ubongo: 240 mg

Minofu: 600 mg

Mafupa, Tsitsi kapena Khungu: 1.5g

● Matenda a nyamakazi:

Kulemera kwake: 9g

Krustacea: 450 mg

● Mwazi wonse:2 machubu

● Maselo: 106 maselo

● Seramu ndi Plasma:6 mL pa

Kutumiza Kwachitsanzo Kovomerezeka

Chotengera:
2 ml centrifuge chubu (Tin zojambulazo sizovomerezeka)
Zitsanzo zolembera: Gulu+ lofanana mwachitsanzo A1, A2, A3;B1, B2, B3...

Kutumiza:
1.Dry-ice: Zitsanzo ziyenera kuikidwa m'matumba ndikukwiriridwa mu ayezi wouma.
2.RNAstable machubu: RNA zitsanzo akhoza zouma mu RNA kukhazikika chubu (mwachitsanzo RNAstable®) ndi kutumizidwa mu firiji.

Kuyenda kwa Ntchito Yantchito

Chitsanzo cha QC

Kuyesera kupanga

kutumiza chitsanzo

Kupereka zitsanzo

Kuyesera koyendetsa ndege

Kusintha kwa RNA

Kukonzekera kwa Library

Kumanga laibulale

Kutsata

Kutsata

Kusanthula deta

Kusanthula deta

Pambuyo pogulitsa Services

Pambuyo pogulitsa ntchito


  • Zam'mbuyo:
  • Ena:

  • Bioinformatics

    wps_doc_14

    Kuzindikiritsa miRNA: kapangidwe ndi kuya

     

     

     miRNA-precursor-mapangidwe-ndi-kutsata-kuya

     

    Kufotokozera kosiyana kwa miRNA - magulu a hiearchical clustering

    图片34

     

    Kufotokozera kogwira ntchito kwa chandamale cha ma miRNA owonetsedwa mosiyanasiyana

    Chithunzi cha 35

    Onani kupita patsogolo kwa kafukufuku komwe kumayendetsedwa ndi BMKGene 'sRNA yotsatizanatsatizana ndi zofalitsa.

      

    Chen, H. et al.(2023) 'Matenda a Viral amalepheretsa saponin biosynthesis ndi photosynthesis ku Panax notoginseng', Plant Physiology and Biochemistry, 203, p.108038. doi: 10.1016/J.PLAPHY.2023.108038.

    Li, H. et al.(2023) ' Chomera cha FYVE chokhala ndi puloteni ya FREE1 chimagwirizana ndi zigawo za microprocessor kuti zipondereze miRNA biogenesis ', Malipoti a EMBO, 24(1).doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.

    Yu, J. et al.(2023) 'The MicroRNA Ame-Bantam-3p Controls Larval Pupal Development by Targeting the Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) in the Honeybee, Apis mellifera', International Journal of Molecular Sciences, 24(6), p. .5726. doi: 10.3390/IJMS24065726/S1.

    Zhang, M. et al.(2018) 'Integrated Analysis of MiRNA and Genes Associated With Meat Quality Amasonyeza kuti Gga-MiR-140-5p Imakhudza Kuyika kwa Mafuta mu Nkhuku', Cellular Physiology ndi Biochemistry, 46 (6), pp. 2421-2433.doi: 10.1159/000489649.

    pezani mtengo

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