Small RNA Sequencing-Illumina
Mawonekedwe
● Kukonza laibulale yosankha kukula kwake.
● Kusanthula kwa Bioinformamatic kunakhazikika pa kulosera kwa miRNA ndi zolinga.
Ubwino wa Utumiki
●Katswiri Wazambiri: Takonza zitsanzo zopitilira 300, zokhala ndi mitundu yosiyanasiyana. Timabweretsa luso laukadaulo pantchito iliyonse.
●Ulamuliro Wabwino Kwambiri: Timagwiritsa ntchito mfundo zazikuluzikulu pamagawo onse, kuyambira kukonzekera zitsanzo mpaka kukonzekera laibulale, kutsatizana ndi bioinformatics. Kuwunika kwathu mosamalitsa kumatsimikizira kuperekedwa kwa zotsatira zabwino nthawi zonse.
●Kusanthula kwathunthu kwa bioinformatics:Imathandizira kuzindikira ma miRNA onse odziwika komanso atsopano, kuzindikiritsa zolinga za miRNAs, komanso kumasulira kofananira ndikulemeretsa ndi ma database angapo (KEGG, GO).
●Thandizo la Post-Sales: Timamvetsetsa kufunikira kopezeka, ndichifukwa chake kudzipereka kwathu kumapitilira kutha kwa ntchito ndi miyezi itatu mutagulitsa. Panthawiyi, timapereka zotsatila za polojekiti, chithandizo chazovuta, ndi magawo a Q&A kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira.
Zitsanzo Zofunika ndi Kutumiza
| Library | nsanja | Deta yovomerezeka | Zithunzi za QC |
| Kukula kwasankhidwa | Illumina SE50 | 10M-20M imawerengedwa | Q30≥85% |
Zitsanzo Zofunika:
Nucleotides:
| Conc.(ng/μl) | Mtengo (μg) | Chiyero | Umphumphu |
| ≥80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Zochepa kapena zopanda mapuloteni kapena DNA kuipitsidwa komwe kumawonetsedwa pa gel. | RIN≥6.0; 5.0≥28S/18S≥1.0; kukwera kochepa kapena kopanda koyambira |
● Zomera:
Muzu, tsinde kapena petal: 450 mg
Tsamba kapena Mbewu: 300 mg
Chipatso: 1.2 g
● Chinyama:
Mtima kapena matumbo: 450 mg
Viscera kapena Ubongo: 240 mg
Minofu: 600 mg
Mafupa, Tsitsi kapena Khungu: 1.5g
● Matenda a nyamakazi:
Kulemera kwake: 9g
Krustacea: 450 mg
● Mwazi wonse:2 machubu
● Maselo: 106 maselo
● Seramu ndi Plasma:6 ml pa
Kutumiza Kwachitsanzo Kovomerezeka
Chidebe: 2 ml centrifuge chubu (zojambula za malata ndizosavomerezeka)
Zitsanzo zolembera: Gulu+ lofanana mwachitsanzo A1, A2, A3; B1, B2, B3.
Kutumiza:
1. Dry-ice: Zitsanzo ziyenera kupakidwa m'matumba ndikukwiriridwa mu ayezi wouma.
2. RNAstable chubu: RNA zitsanzo akhoza zouma mu RNA kukhazikika chubu (mwachitsanzo RNAstable®) ndi kutumizidwa mu firiji.
Kuyenda kwa Ntchito Yantchito
Kuyesera kupanga
Kupereka zitsanzo
Kusintha kwa RNA
Kumanga laibulale
Kutsata
Kusanthula deta
Pambuyo pogulitsa ntchito
Bioinformatics
● Kuwongolera khalidwe la data yaiwisi
● Gulu laling'ono la RNA
● Kufotokozera kwa miRNA ndi kusanthula kaganizidwe kosiyana
● miRNA chandamale jini ndi mosiyanasiyana miRNA chandamale jini
● Kafotokozedwe ka jini ndi kuchulukitsidwa kosiyanasiyana kwa jini ya miRNA
Kuzindikiritsa miRNA: kapangidwe ndi kuya
Kufotokozera kosiyana kwa miRNA - magulu a hiearchical clustering
Kufotokozera kogwira ntchito kwa chandamale cha ma miRNA owonetsedwa mosiyanasiyana
Onani kupita patsogolo kwa kafukufuku komwe kumayendetsedwa ndi BMKGene 'sRNA yotsatizanatsatizana ndi zofalitsa.
Chen, H. et al. (2023) 'Matenda a virus amalepheretsa saponin biosynthesis ndi photosynthesis ku Panax notoginseng',Plant Physiology ndi Biochemistry, 203, p. 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) ' Chomera cha FYVE chokhala ndi puloteni FREE1 chimagwirizana ndi zigawo za microprocessor kuti zithetse miRNA biogenesis ',Malipoti a EMBO, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al. (2023) 'MicroRNA Ame-Bantam-3p Imayang'anira Kukula kwa Mphuphu ya Larval Poyang'ana Magawo 8 a Gene (megf8) mu Honeybee, Apis mellifera',International Journal of Molecular Sciences, 24(6), p. 5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al. (2018) 'Integrated Analysis of MiRNA and Genes Associated with Meat Quality Awulula kuti Gga-MiR-140-5p Imakhudza Kuyika Kwamafuta Opangidwa mu Intramuscular mu Nkhuku', (2018)Ma Cellular Physiology ndi Biochemistry, 46(6), tsamba 2421-2433. doi: 10.1159/000489649.
