Small RNA Sequencing-Illumina
Mawonekedwe
● Kukonzekera laibulale kumaphatikizapo kusankha kukula kwake
● Kusanthula kwa Bioinformamatic kunakhazikika pa kulosera kwa miRNA ndi zolinga zawo
Ubwino wa Utumiki
●Kusanthula kwathunthu kwa bioinformatics:Kuthandizira kuzindikirika kwa ma miRNA onse odziwika komanso atsopano, kuzindikiritsa zolinga za miRNAs, komanso mawu ofananirako ogwira ntchito ndikulemeretsa ndi ma database angapo (KEGG, GO)
●Kuwongolera Kwabwino Kwambiri: Timagwiritsa ntchito mfundo zazikuluzikulu pamagawo onse, kuyambira pakukonza zachitsanzo ndi laibulale mpaka kutsatizana ndi bioinformatics. Kuyang'anitsitsa mosamalitsa kumeneku kumatsimikizira kuperekedwa kwa zotsatira zabwino nthawi zonse.
●Thandizo la Post-Sales: Kudzipereka kwathu kumapitilira kutha kwa projekiti ndi nthawi ya miyezi itatu mutagulitsa. Panthawiyi, timapereka zotsatila za polojekiti, chithandizo chazovuta, ndi magawo a Q&A kuti tiyankhe mafunso aliwonse okhudzana ndi zotsatira.
●Katswiri Wazambiri: Ndi mbiri yakutseka bwino mapulojekiti angapo a sRNA okhala ndi mitundu yopitilira 300 m'magawo osiyanasiyana ofufuza, gulu lathu limabweretsa zodziwa zambiri pantchito iliyonse.
Zitsanzo Zofunika ndi Kutumiza
| Library | nsanja | Deta yovomerezeka | Zithunzi za QC |
| Kukula kwasankhidwa | Illumina SE50 | 10M-20M imawerengedwa | Q30≥85% |
Zitsanzo Zofunika:
Nucleotides:
| Conc.(ng/μl) | Mtengo (μg) | Chiyero | Umphumphu |
| ≥80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Zochepa kapena zopanda mapuloteni kapena DNA kuipitsidwa komwe kumawonetsedwa pa gel. | RIN≥6.0; 5.0≥28S/18S≥1.0; kukwera kochepa kapena kopanda koyambira |
● Zomera:
Muzu, tsinde kapena petal: 450 mg
Tsamba kapena Mbewu: 300 mg
Chipatso: 1.2 g
● Chinyama:
Mtima kapena matumbo: 450 mg
Viscera kapena Ubongo: 240 mg
Minofu: 600 mg
Mafupa, Tsitsi kapena Khungu: 1.5g
● Matenda a nyamakazi:
Kulemera kwake: 9g
Krustacea: 450 mg
● Mwazi wonse:2 machubu
● Maselo: 106 maselo
● Seramu ndi Plasma:6 ml pa
Kutumiza Kwachitsanzo Kovomerezeka
Chidebe: 2 ml centrifuge chubu (zojambula za malata ndizosavomerezeka)
Zitsanzo zolembera: Gulu+ lofanana mwachitsanzo A1, A2, A3; B1, B2, B3.
Kutumiza:
1. Madzi owuma: Zitsanzo ziyenera kupakidwa m'matumba ndikukwiriridwa mu ayezi wouma.
2. RNAstable chubu: RNA zitsanzo akhoza zouma mu RNA kukhazikika chubu (mwachitsanzo RNAstable®) ndi kutumizidwa mu firiji.
Kuyenda kwa Ntchito Yantchito
Kuyesera kupanga
Kupereka zitsanzo
Kusintha kwa RNA
Kumanga laibulale
Kutsata
Kusanthula deta
Pambuyo pogulitsa ntchito
Bioinformatics
● Kuwongolera khalidwe la data yaiwisi
● gulu la sRNA
● Kuyanjanitsa ku genome
● Kuzindikiritsa miRNA yodziwika komanso yatsopano
● Kusiyana kwa mawu a miRNA
● Ndemanga zogwirira ntchito za miRNA targets
Kuzindikiritsa miRNA: kapangidwe ndi kuya
Kufotokozera kosiyana kwa miRNA - magulu a hiearchical clustering
Kufotokozera kogwira ntchito kwa chandamale cha ma miRNA owonetsedwa mosiyanasiyana
Onani kupita patsogolo kwa kafukufuku komwe kumayendetsedwa ndi BMKGene 'sRNA yotsatizanatsatizana ndi zofalitsa.
Chen, H. et al. (2023) 'Matenda a Viral amalepheretsa saponin biosynthesis ndi photosynthesis ku Panax notoginseng', Plant Physiology and Biochemistry, 203, p. 108038. doi: 10.1016/J.PLAPHY.2023.108038.
Li, H. et al. (2023) ' Chomera cha FYVE chokhala ndi puloteni ya FREE1 chimagwirizana ndi zigawo za microprocessor kuti zipondereze miRNA biogenesis ', Malipoti a EMBO, 24(1). doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.
Yu, J. et al. (2023) 'The MicroRNA Ame-Bantam-3p Controls Larval Pupal Development by Target the Multiple Epidermal Growth Factor-like Domains 8 Gene (megf8) in the Honeybee, Apis mellifera', International Journal of Molecular Sciences, 24(6), p. . 5726. doi: 10.3390/IJMS24065726/S1.
Zhang, M. et al. (2018) 'Integrated Analysis of MiRNA and Genes Associated with Meat Quality Awulula kuti Gga-MiR-140-5p Imakhudza Kuyika kwa Mafuta a Intramuscular mu Nkhuku', Cellular Physiology ndi Biochemistry, 46 (6), pp. 2421-2433. doi: 10.1159/000489649.
