BMKCloud Log in
条形 banner-03

Zogulitsa

Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Ma genotyping apamwamba kwambiri, makamaka pa anthu ochuluka, ndi gawo lofunikira kwambiri pa maphunziro a ma genetic, omwe amapereka maziko a chibadwa cha kutulukira kwa majini, kusanthula kwachisinthiko, ndi zina zotero. ) imayambitsidwa pofuna kuchepetsa mtengo wotsatizanatsatizana pachitsanzo chilichonse, komanso kukhalabe ndi luso lokwanira pakutulukira kwa chibadwa.Izi nthawi zambiri zimatheka pochotsa chidutswa choletsa mkati mwa kukula kwake, chomwe chimatchedwa laibulale yoyimira (RRL).Specific-locus amplified fragment sequencing (SLAF-Seq) ndi njira yodzipangira yokha ya SNP genotyping yokhala ndi kapena popanda genome.
Pulatifomu: Illumina NovaSeq Platform


Tsatanetsatane wa Utumiki

Zotsatira za Demo

Zofalitsa Zowonetsedwa

Tsatanetsatane wa Utumiki

Technical Scheme

111

Kuyenda kwa ntchito

流程图

Ubwino wa Utumiki

Kuzindikira kwakukulu kwa chikhomo- Ukadaulo wotsogola wotsogola kwambiri umathandizira SLAF-Seq kupeza ma tag mazana masauzande mu genome yonse.

Kudalira kochepa pa genome- Itha kugwiritsidwa ntchito ku mitundu yokhala ndi kapena popanda genome.

Flexible scheme design- Single-enzyme, ma enzyme awiri, chimbudzi cha ma enzyme ambiri ndi mitundu yosiyanasiyana ya michere, zonse zitha kusankhidwa kuti zikwaniritse cholinga chofufuza kapena mitundu yosiyanasiyana.Kuunikiratu mu silico kumagwiritsidwa ntchito kutsimikizira kapangidwe kabwino ka enzyme.

Kuchita bwino kwa enzymatic chimbudzi- Kuyesera kusanachitike kunachitika kuti kukhathamiritse mikhalidwe, zomwe zimapangitsa kuyesa kokhazikika kukhala kokhazikika komanso kodalirika.Kutolere bwino kwa zidutswa kumatha kukwaniritsa 95%.

Ma tag a SLAF omwe amagawidwa mofanana- Ma tag a SLAF amagawidwa mofanana mu ma chromosome onse kumlingo waukulu, kukwaniritsa avareji ya 1 SLAF pa 4 kb.

Kupewa koyenera kubwereza- Kubwerezabwereza mu data ya SLAF-Seq kumachepetsedwa mpaka 5%, makamaka mumitundu yokhala ndi kubwereza kwakukulu, monga tirigu, chimanga, ndi zina.

Zochitika zambiri-Kupitilira 2000 ntchito zotseka za SLAF-Seq pazamoyo mazanamazana zomwe zimaphimba zomera, zoyamwitsa, mbalame, tizilombo, zamoyo zam'madzi, ndi zina zambiri.

Ntchito yodzipangira yokha bioinformatics- Integrated bioinformamatic workflow for SLAF-Seq idapangidwa ndi BMKGENE kuti zitsimikizire kudalirika komanso kulondola kwa zotsatira zomaliza.

 

Mafotokozedwe a Utumiki

 

nsanja

Conc.(ng/gl)

Zonse (ug)

OD260/280

Illumina NovaSeq

>35

>1.6(Chigawo> 15μl)

1.6-2.5

Zindikirani: Zitsanzo zitatu, iliyonse ili ndi ma enzyme atatu, idzachitidwa kuti ayesedwe kale.

Njira Yotsatirira Yolangizidwa

Kutsata kuya: 10X/Tag

Kukula kwa Genome

Analimbikitsa SLAF Tags

<500Mb

100K kapena WGS

500 Mb-1 Gb

100 k

1 Gb -2 Gb

200 k

Ma genome akuluakulu kapena ovuta

300-400K

 

Mapulogalamu

 

Analimbikitsa

Chiwerengero cha Anthu

 

Kutsata ndondomeko ndi kuya

 

Kuzama

 

Nambala ya Tag

 

GWAS

 

Nambala yachitsanzo ≥200

 

10x pa

 

 

 

 

 

Malinga ndi

kukula kwa genome

 

Kusintha kwa Genetic

 

Anthu payekhapayekha

gulu ≥ 10;

zitsanzo zonse ≥ 30

 

10x pa

 

Kutumiza Kwachitsanzo Kovomerezeka

Chidebe: 2 ml centrifuge chubu

Pazitsanzo zambiri, timalimbikitsa kuti musasunge ethanol.

Zitsanzo zolembetsera: Zitsanzo ziyenera kulembedwa momveka bwino komanso zofanana ndi zomwe zatumizidwa.

Kutumiza: Owuma-Izi: Zitsanzo ziyenera kulongedzedwa m'matumba kaye ndikukwiriridwa mu ayezi wouma.

Njira ya utumiki

Chitsanzo cha QC
Kuyesera koyendetsa ndege
Kuyesa kwa SLAF
Kukonzekera kwa Library
Kutsata
Kusanthula deta
Pambuyo pogulitsa Services

Chitsanzo cha QC

Kuyesera koyendetsa ndege

SLAF-kuyesera

Kukonzekera kwa Library

Kutsata

Kusanthula Zambiri

Pambuyo-kugulitsa Services


  • Zam'mbuyo:
  • Ena:

  • 1. Ziwerengero za zotsatira za mapu

    chithunzi1

    A1

    2. Kukula kwa chikhomo cha SLAF

    A2

    3. Mawu osinthika

    A3

    Chaka

    Journal

    IF

    Mutu

    Mapulogalamu

    2022

    Kulankhulana kwachilengedwe

    17.694

    Genomic maziko a giga-chromosomes ndi giga-genome ya mtengo peony

    Paeonia ostii

    SLAF-GWAS

    2015

    Phytologist watsopano

    7.433

    Zolemba zapakhomo zimakhazikitsa madera a genomic ofunika kwambiri mu agronomic

    soya

    SLAF-GWAS

    2022

    Journal of Advanced Research

    12.822

    Ma genome-wide artificial introgressions of Gossypium barbadense into G. hirsutum

    wonetsani malo apamwamba kuti muwongolere nthawi imodzi yamtundu wa thonje komanso zokolola

    makhalidwe

    SLAF-Evolutionary genetics

    2019

    Chomera cha Molecular

    10.81

    Population Genomic Analysis ndi De Novo Assembly Reveal Origin of Weedy

    Mpunga ngati Masewera a Chisinthiko

    SLAF-Evolutionary genetics

    2019

    Nature Genetics

    31.616

    Mndandanda wa ma genome ndi mitundu yosiyanasiyana ya ma carp wamba, Cyprinus carpio

    Mapu a SLAF-Linkage

    2014

    Nature Genetics

    25.455

    Ma genome a mtedza wolimidwa amapereka chidziwitso cha legume karyotypes, polyploid

    chisinthiko ndi kubzala mbewu.

    Mapu a SLAF-Linkage

    2022

    Plant Biotechnology Journal

    9.803

    Kuzindikirika kwa ST1 kumawonetsa kusankha komwe kumakhudza kukwera kwa mbeu

    ndi mafuta ochulukirapo panthawi yoweta soya

    Kukula kwa SLAF-Marker

    2022

    International Journal of Molecular Sciences

    6.208

    Kuzindikiritsa ndi DNA Marker Development for Wheat-Leymus mollis 2Ns (2D)

    Kusintha kwa Disomic Chromosome

    Kukula kwa SLAF-Marker

    pezani mtengo

    Lembani uthenga wanu apa ndikutumiza kwa ife

    Titumizireni uthenga wanu: