Tgude s16 nucle nyukiliya acid extractor
Kuyambitsa Zoyambitsa
Tgude S16 Nyukiliya acid Exortractor ikhoza kukhala ndi zitsanzo zingapo nthawi imodzi ndi ndodo zamagetsi kuti azimangirira maginito, katoni kabwino ka magiriji kapena maginisi. Pogwiritsa ntchito zofananira za acid acid exent, izi zimatha kuyeretsa DNA / RNA kuchokera ku zitsanzo za nyama, chomera, ma buki., ndikutulutsa gelr.
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Mawonekedwe
Super yosavuta kugwiritsa ntchito
Palibe ntchito yowonjezera yowonjezera. Makina ogulitsa amatha kusinthidwa monga amafunikira.
Kukhazikitsa ndi ntchito kumafuna maphunziro ochepa. Ndi mapulogalamu okhazikitsidwa ndi asanaikidwe, tengani cartridge, sankhani protocol ndikuyendetsa. Mapulogalamu amakhala okonzeka kugwiritsa ntchito bwino komanso kuthekera.
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Njira Yatsopano
Ndodo yamagetsi imatengera njira yolimba yamphamvu.
Mikanda yamatsenga imamangirira pansi ndodo, yomwe imatsimikizira kuti luntha limatha kubisa mikanda yonse yamagetsi ngakhale voliyumu yoyenerera ndi yaying'ono. Mapangidwe amakulitsa zokolola za exvac acid.
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Kupanga koyenera kwa thanki yotentha
Pali mzere womwe umayikidwa pakati pa buluzi wa lysis ndi mzere wamphamvu, zomwe zimalepheretsa bwino kuti zisawonongeke chifukwa cha mtundu wa nduna. Chifukwa chake, kuwongolera voliyumu ya nyukiliya acid yankho ndi lolondola.
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Kuwongolera kuwongolera ndi chitetezo chamadongosolo
Itha kuyikidwa pa benchi oyera kapena mu hood yamankhwala.
Chidacho chikuyenda munjira zokha komanso kutsekedwa.
Makina owongolera amatengera njira ziwiri: njira yowonongeka yamkati ndi ma ultraviolelet sriluzazation dongosolo, kuti apewe kuipitsidwa ndi mizati ndi zitsanzo.
Onjezerani ma reagents komanso zotayika zotayika.
Kuchotsa mankhwala kumatha kuthetsedwa kwakukulu mpaka kuwonetsetsa chitetezo cha opareshoni.
Zotsatira za Demo
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Zoyeserera: Kugwiritsa ntchito Tgude S16 yokhala ndi FLBOGENS TRAIDE HARCHALL DNAAl DNA Kit (4993704)
Kutulutsa zokhazokha za genimic DNA ya zitsanzo zingapo kuphatikiza maselo, minofu, magazi, malo owuma Magazi (D.), Lactobacillus, E.COB, Kukwaniritsa Zokolola Zabwino ndi Zoyera.
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Od260 / Od280 kuzungulira 1.7 ~ 1.9, ndipo
Ode260 / Od230> 1.6.
Voliyumu: 100 μl
Agarose gel ndende: 1.5%
Kutsitsa voliyumu: 1 μl
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Kugwiritsa ntchito Tgude S16 yokhala ndi Fliment Smart Viral DNA / RNA Kit (4993702)
Kutulutsa DNA / RNA kuchokera ku magawo angapo kuphatikiza magazi athunthu, swab ndi plasma adapeza zokolola zabwino komanso zoyera. -
Od260 / Od280 kuzungulira 1.7 ~ 1.9, ndi ode260 / Od230> 1.6.
Kampani & B kampani: Brans wodziwika bwino.
ND: Matenda a chideru
Kumva kocheperako kumatha kufikira 100 makope / ml.
Zoyeserera: Kugwiritsa ntchito Tgude S16 ndi Regent TRUIDE BLUIDE BLONG TRAIME BLAICK
Od260 / Od280 kuzungulira 1.7 ~ 1.9, ndi ode260 / Od230> 1.7.
Gwero: Zitsanzo zitatu za magazi. Ma rectics atatu akuyesa kwa chikho.
Kuwerenga pakhungu lililonse kumachokera kumodzi yomweyo.
Mbiri Yachitsanzo: Magazi Oundana
Makina Othandizira: 200 μl
Voliyumu: 100 μl
Agarose gel ndende: 1.5%
Kutsitsa voliyumu: 1 μl
M: Marker III, Tiangen
Kampani & b: zodziwika bwino
2000 BP gawo la Agarose gel DNA kuyeretsedwa ndi kuchira
Agarose gel ndende: 1.5% (TB)
Kutsitsa Voliyumu: 8 μl
Marker: D2000, Tiangn
S16-01, S16-02, ndi S16-03 ikuyimira chiwonetsero chazofanana ndi zoyeserera za acid 3 Tgude Stictor Detorict Kit (4993550) nthawi yomweyo.
Kampani: Bukuli limapindika-cholembera chida chodziwika bwino
Agilent 2100 adagwiritsidwa ntchito pozindikira RNA kutchuka kwa RNA.
Studges: mbewa ya CD-1
Mbiri Yachitsanzo: Mwatsopano Watsopano
Kukula Kwachitsanzo: 200 μl
Zoyesereratu: RNA yonse yomwe imachotsedwa magazi ndi Tgude SC16 yokhala ndi Scaide Scride magazi / cell / chiyero chabwino, ndipo amatha kukwaniritsa zofuna zotsika kwambiri.
Kutsitsa voliyumu: 1 μl. Chiwindicho chidasungunuka kasanu ndikudzaza. Agarose gel ndende: 1%. Electrophores kwa 20 min pa 6 v / cm;
M: Tiangn Marker III
1-4: Mtima 5-8: Chiwindi 9-12: Lung 13-16: Impso
Zitsanzo ziwiri zoyambirira za minofu iliyonse zimachotsedwa ndi kuzungulira kwa mzere wozungulira, ndipo awiri omaliza adachotsedwa zokha ndi Tgude S16.
Studges: mbewa ya CD-1
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Zotsatira Zochitika: Nyuzi ya nyama ya nyama yam'madzi DNA imangotengedwa ndi Tguides S16 yokhala ndi asgude a TNAIDE TRAIDID TOGNIDID Magneide (4993547) ali ndi protocol yozungulira. Kuyera kumakhala chimodzimodzi, ndi ode260 / od280 kuzungulira 1.7 ~ 1.9 ndi ode260 / od230> 1.7. Pomaliza, Tgude S16 itha kugwiritsidwa ntchito pochotsa nyamayo kuchokera ku nyama ya nyamayo m'malo mwa mzere wozungulira.
Kutulutsa kwa RNA zonse kuchokera ku zitsanzo zosiyanasiyana
Kukula Kwachitsanzo: 100 mg
Chithandizo cha Prey Pre-Mankhwala: Kutentha kochepa
Agarose gel ndende: 1% (tae)
Kutsitsa voliyumu: 1 μl
M: Marker III, Tiangen
1-4: mbewu za chimanga 5-8: Chiarn Masamba 9-12: Kabichi Mizu 13-16: Soybeans
Zitsanzo ziwiri zoyambirira zimatulutsidwa ndi gawo lapampheni-podutsira zida ndi zitsanzo ziwiri zomaliza zidatulutsidwa ndi Tguide S16.
Genomic DNA m'chotsani mizu ya tirigu
Kukula Kwachitsanzo: 100 mg
Chithandizo cha Chitsanzo: Kupera ndi Nitrogen Itrogen kapena minofu yopukutira homogenizer
Agarose gel ndende: 1% (tae)
Kutsitsa voliyumu: 2 μl
Marker: D15000, Tiangn
Kampani: mtundu wodziwika bwino
Zoyeserera: Kugwiritsa ntchito Tgude S16 ndi Felivent TRAIDE HEGNIDIC HAMNITIC HARD (4993548) kuti atulutse zokolola zabwino, zomwe zikufanana ndi zokolola zabwino komanso zoyera. Komanso, kukhulupirika kwa genomic DNA kuli bwino kuposa momwe ma makampani amakampani ena amapirira. Poyesa iyi, pafupifupi 15 μg ya nuclectic acid adachotsedwa pamizu 100 mg ya tirigu, ndi ode260 / Od280, ndi Od260> 2.0.
