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Zogulitsa

Kutsatizana konse kwa genome bisulfite

DNA methylation pamalo achisanu mu cytosine (5-mC) imakhudza kwambiri mafotokozedwe a majini ndi zochitika zama cell.Ma methylation osadziwika bwino amalumikizidwa ndi mikhalidwe ndi matenda angapo, monga khansa.WGBS yakhala muyezo wagolide wophunzirira genome-wide methylation pa single base resolution.

Pulatifomu: Illumina NovaSeq Platform


Tsatanetsatane wa Utumiki

Bioinformatics

Zotsatira za Demo

Ubwino wa Utumiki

● Pulatifomu yathunthu: perekani ntchito imodzi yabwino kwambiri kuyambira pakukonza zitsanzo, kumanga laibulale, kutsata kusanthula kwa bioinformatics.

● Kulondola kwakukulu: Ukadaulo wokhwima wa kutembenuka kwa methylation ungathe kusanthula molondola mkhalidwe wa methylation wa C base imodzi.

● Kufalikira kwakukulu: kuzindikira malo a methylation pamlingo wa genome-wide.

● Kubwereza kwabwino: kukonza kokhazikika, koyenera kusanthula kofananitsa pakati pa zitsanzo zambiri.

 

Zitsanzo Zofotokozera

 

nsanja

 

 

Werengani Utali

 

 

Njira Yotsatirira

 

Ilumina

Mtengo wa 150PE

30X, zofananira zitatu zamoyo, zokhala ndi ma genome apamwamba kwambiri

Zitsanzo Zofunika

 

Mtundu Wachitsanzo

 

 

Mtengo (μg)

 

 

Conc.(ng/μl)

 

 

Kuchuluka (μl)

 

 

Chiyero

 

gDNA

≥ 2

≥ 20

 

≥ 20

 

Kuwonongeka kochepa ndi kuipitsidwa

 

 

 

Kuyenda kwa Ntchito Yantchito

kutumiza chitsanzo

Kupereka zitsanzo

Kuyesera koyendetsa ndege

Kutulutsa kwa DNA

Kukonzekera kwa Library

Kumanga laibulale

Kutsata

Kutsata

Kusanthula deta

Kusanthula deta

数据上传-01

Kutumiza kwa data


  • Zam'mbuyo:
  • Ena:

  •  Bioinformatics

    chithunzi1

    1.Genome-wide DNA methylation distribution

    3(1)

    2. Ndemanga pa dera la CGI lomwe lili ndi methylated kwambiri

    4(1)

    pezani mtengo

    Lembani uthenga wanu apa ndikutumiza kwa ife

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