OEM Manufacturer Oxford Nanopore Service - De novo Full-length Transcriptome sequencing -PacBio – Biomarker
OEM Manufacturer Oxford Nanopore Service - De novo Full-length Transcriptome sequencing -PacBio – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data processing
ü Transcript identification
ü Sequence structure
ü Expression Quantification
ü Function Annotation
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Amount | Total ≥ 3 μg; Conc. ≥ 300 ng/μl; Volumn ≥ 10 μl |
| Integrity | For plants: RIN ≥ 7.5; For animals: RIN ≥ 8; 28S/18S ≥ 1.0; Limited or no baseline elevation |
| Purity | OD260/280 ≥ 1.8; OD260/230 ≥ 1.0; Clear peak at 260 nm |
| Note | Protein or DNA contamination is very likely to affect the measurements and the samples’ QC level. |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 – 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
Our business sticks for the basic principle of "Quality could be the life with the firm, and track record will be the soul of it" for OEM Manufacturer Oxford Nanopore Service - De novo Full-length Transcriptome sequencing -PacBio – Biomarker , The product will supply to all over the world, such as: Lesotho , Turkmenistan , Jeddah , Our company insists on the purpose of "takes service priority for standard, quality guarantee for the brand, do business in good faith, to provide professional, rapid, accurate and timely service for you". We welcome old and new customers to negotiate with us. We will serve you with all sincerity!
By Joanne
from Puerto Rico
- 2017.06.25 12:48
In China, we have purchased many times, this time is the most successful and most satisfactory, a sincere and realiable Chinese manufacturer!
By Agatha
from Portland
- 2017.05.21 12:31
