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Personlized Products Microbial Genome - circRNA sequencing – Illumina – Biomarker

Service Details

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As for aggressive price ranges, we believe that you will be searching far and wide for anything that can beat us. We can easily state with absolute certainty that for such high-quality at such price ranges we're the lowest around for Rna Sequencing , Hifi Sequencing Service , Plant Bioinformatics , Seeing believes! We sincerely welcome the new clients abroad to build organization associations and also hope to consolidate the associations while using the long-established prospects.

Personlized Products Microbial Genome - circRNA sequencing – Illumina – Biomarker Detail:

Service Advantages

Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity	Integrity	Contamination	Amount
OD260/280≥1.7-2.5;OD260/230≥0.5-2.5;	For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation	Limited or no protein or DNA contamination shown on gel.	Conc. ≥100 ng/μl; Volumn ≥ 10 μl; Total ≥ 2 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×10⁷
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×10⁵, flash frozen in liquid nitrogen is recommended.

Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)

Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:
Dry-ice: Samples need to be packed in bags and buried in dry-ice.
RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services

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