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Professional China 16s Rrna Sequencing Provider -
Eukaryotic mRNA sequencing – Illumina – Biomarker Detail:
Advantages
Sample Requirements and Delivery
| Library |
Sequencing strategy |
Data recommended |
Quality Control |
| Poly A enriched |
Illumina PE150 |
6 Gb
|
Q30≥85% |
Sample Requirements:
Nucleotides:
| Purity |
Integrity |
Amount |
| OD260/280≥1.7-2.5 OD260/230≥0.5-2.5Limited or no protein or DNA contamination shown on gel. |
For plants: RIN≥6.5;For animals: RIN≥7;28S/18S≥1.0;limited or no baseline elevation |
Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
- Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow

Experiment design

Sample delivery

RNA extraction

Library construction

Sequencing

Data analysis

After-sale services
Product detail pictures:
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With a complete scientific quality management system, good quality and good faith, we win good reputation and occupied this field for
Professional China 16s Rrna Sequencing Provider -
Eukaryotic mRNA sequencing – Illumina – Biomarker , The product will supply to all over the world, such as: Saudi Arabia
, Bolivia
, Swiss
, We pursue the management tenet of "Quality is superior, Service is supreme, Reputation is first", and will sincerely create and share success with all clients. We welcome you to contact us for more information and look forward to working with you.