Professional Design Dna Methylation Sequencing - De novo Full-length Transcriptome sequencing -PacBio – Biomarker
Professional Design Dna Methylation Sequencing - De novo Full-length Transcriptome sequencing -PacBio – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data processing
ü Transcript identification
ü Sequence structure
ü Expression Quantification
ü Function Annotation
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Amount | Total ≥ 3 μg; Conc. ≥ 300 ng/μl; Volumn ≥ 10 μl |
| Integrity | For plants: RIN ≥ 7.5; For animals: RIN ≥ 8; 28S/18S ≥ 1.0; Limited or no baseline elevation |
| Purity | OD260/280 ≥ 1.8; OD260/230 ≥ 1.0; Clear peak at 260 nm |
| Note | Protein or DNA contamination is very likely to affect the measurements and the samples’ QC level. |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 – 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Microorganism: Mass ≥ 1 g
Recommended Sample Delivery
Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment:
1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
Innovation, excellent and reliability are the core values of our business. These principles today extra than ever form the basis of our success as an internationally active mid-size company for Professional Design Dna Methylation Sequencing - De novo Full-length Transcriptome sequencing -PacBio – Biomarker , The product will supply to all over the world, such as: Bangkok, Norwegian, Argentina, For more than ten years experience in this filed, our company has gained high reputation from home and abroad. So we welcome friends from all over the world to come and contact us, not only for business, but also for friendship.
By Jerry from Salt Lake City - 2018.05.22 12:13
The product manager is a very hot and professional person, we have a pleasant conversation, and finally we reached a consensus agreement.
By Dorothy from European - 2017.08.18 11:04
