Professional Design Dna Methylation Sequencing - Full-length mRNA sequencing -Nanopore – Biomarker
Professional Design Dna Methylation Sequencing - Full-length mRNA sequencing -Nanopore – Biomarker Detail:
Service Advantages
Service Specifications
Bioinformatics analyses
ü Raw data processing
ü Transcript identification
ü Alternative splicing
ü Expression quantification in gene level and isoform level
ü Differential expression analysis
ü Function annotation and enrichment (DEGs and DETs)
Sample Requirements and Delivery
Sample Requirements:
Nucleotides:
| Purity | Integrity | Amount |
| OD260/280≥1.8；OD260/230≥1.0；Clear peak at 260 nmLimited or no protein or DNA contamination shown on gel. | For plants: RIN≥7.5;For animals: RIN≥8;28S/18S≥1.0;limited or no baseline elevation | Conc. ≥40 ng/μl;Total ≥ 1 μg |
Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.
Cell suspension: Cell count = 3×106 - 1×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended, which is preferable for micro extraction.
Blood samples: Volume≥1 ml
Recommended Sample Delivery
Container: 2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …
Shipment: 1. Dry-ice: Samples need to be packed in bags and buried in dry-ice.
- RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.
Service Work Flow
Sample delivery
Library construction
Sequencing
Data analysis
After-sale services
Service Work Flow
Experiment design
Sample delivery
RNA extraction
Library construction
Sequencing
Data analysis
After-sale services
Product detail pictures:
Related Product Guide:
With advanced technologies and facilities, strict quality control, reasonable price, superior service and close co-operation with customers, we are devoted to providing the best value for our customers for Professional Design Dna Methylation Sequencing - Full-length mRNA sequencing -Nanopore – Biomarker , The product will supply to all over the world, such as: Madagascar , Liverpool , South Africa , Our objective is "to supply first step products and best service for our customers, thus we are sure you must have a margin benefit through cooperating with us". If you are interested in any of our products or would like to discuss a custom order, please feel free to contact us. We are looking forward to forming successful business relationships with new clients around the world in the near future.
By Lillian
from San Francisco
- 2018.02.04 14:13
The factory has advanced equipment, experienced staffs and good management level, so product quality had assurance, this cooperation is very relaxed and happy!
By Poppy
from Naples
- 2018.12.14 15:26
