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professional factory for Splicing Events - Non-Reference based mRNA sequencing-Illumina – Biomarker


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professional factory for Splicing Events - Non-Reference based mRNA sequencing-Illumina – Biomarker Detail:

Features

Service Advantages

Sample Requirements and Delivery

Sample Requirements:

Nucleotides:

Purity Integrity Contamination Amount
OD260/280≥1.7-2.5; OD260/230≥0.5-2.5; For plants: RIN≥6.5; For animals: RIN≥7; 28S/18S≥1.0; limited or no baseline elevation Limited or no protein or DNA contamination shown on gel. Conc. ≥30 ng/μl; Volumn ≥ 10 μl; Total ≥ 1.5 μg

Tissue: Weight(dry): ≥1 g
*For tissue smaller than 5 mg, we recommend to send flash frozen(in liquid nitrogen) tissue sample.

Cell suspension: Cell count = 3×107
*We recommend to ship frozen cell lysate. In case that cell counts smaller than 5×105, flash frozen in liquid nitrogen is recommended.

Blood samples:
PA×geneBloodRNATube;
6mLTRIzol and 2mL blood(TRIzol:Blood=3:1)

Recommended Sample Delivery

Container:
2 ml centrifuge tube (Tin foil is not recommended)
Sample labeling: Group+replicate e.g. A1, A2, A3; B1, B2, B3… …

Shipment:
1.Dry-ice: Samples need to be packed in bags and buried in dry-ice.
2.RNAstable tubes: RNA samples can be dried in RNA stabilization tube(e.g. RNAstable®) and shipped in room temperature.

Service Work Flow

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Experiment design

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Sample delivery

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RNA extraction

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Library construction

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Sequencing

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Data analysis

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After-sale services


Product detail pictures:

professional factory for Splicing Events -
 Non-Reference based mRNA sequencing-Illumina – Biomarker detail pictures

professional factory for Splicing Events -
 Non-Reference based mRNA sequencing-Illumina – Biomarker detail pictures

professional factory for Splicing Events -
 Non-Reference based mRNA sequencing-Illumina – Biomarker detail pictures

professional factory for Splicing Events -
 Non-Reference based mRNA sequencing-Illumina – Biomarker detail pictures

professional factory for Splicing Events -
 Non-Reference based mRNA sequencing-Illumina – Biomarker detail pictures

professional factory for Splicing Events -
 Non-Reference based mRNA sequencing-Illumina – Biomarker detail pictures

professional factory for Splicing Events -
 Non-Reference based mRNA sequencing-Illumina – Biomarker detail pictures


Related Product Guide:

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  • The factory has advanced equipment, experienced staffs and good management level, so product quality had assurance, this cooperation is very relaxed and happy!
    5 Stars By Elma from Guatemala - 2017.12.09 14:01
    In general, we are satisfied with all aspects, cheap, high-quality, fast delivery and good procuct style, we will have follow-up cooperation!
    5 Stars By Helen from Portland - 2017.09.16 13:44

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