Key Multi-Omics & Genomics Services
Integrate our high-precision genomics and transcriptomics workflows with advanced pseudotargeted metabolomics to unlock unprecedented system biology insights.
Evolutionary Genetics
Exosomal Small RNA Sequencing-Illumina
Bacterial and Fungal Whole Genome Re-Sequencing
Full-Length mRNA Sequencing-Nanopore
Bridging Discovery and Absolute Precision in Metabolomics
In contemporary life sciences research, understanding the metabolic phenotype serves as the ultimate bridge between genomics, transcriptomics, and biological functional execution. Traditional metabolomics approaches typically force academic researchers into a compromise: Untargeted Metabolomics offers broad chemical coverage but suffers from lower dynamic range and lower quantitative fidelity, whereas classical Targeted Metabolomics yields accurate quantification but is restricted to a small number of predefined reference standards.
To overcome this long-standing methodological bottleneck, Biomarker Technologies (BMKGene) introduces our comprehensive Pseudotargeted Metabolomics Academic Cooperation Framework. Utilizing high-resolution liquid chromatography-mass spectrometry (LC-MS) platforms paired with automated ion-pair selection algorithms and Multiple Reaction Monitoring (MRM) technologies, pseudotargeted metabolomics bridges discovery and absolute quantification. This approach enables large-scale, high-throughput quantitative metabolite profiling across thousands of clinical, agricultural, and environmental samples—delivering high sensitivity, ultra-wide dynamic range, and exceptional batch-to-batch repeatability tailored for high-impact joint academic publishing and translational science.
Broad Coverage & High Sensitivity
Detects thousands of metabolite ion-pairs concurrently without requiring physical pure chemical standards for every targeted analyte prior to screening.
Linear Dynamic Range
Leverages triple quadrupole (QQQ/QTRAP) MRM scanning mode, ensuring robust linear quantification spanning up to 5–6 orders of magnitude.
Multi-Center Cohort Ready
Minimizes matrix retention time shifts and instrument drift, making it ideal for population-scale academic studies and longitudinal clinical trials.
Technological Innovation: How Pseudotargeted Metabolomics Works
Pseudotargeted metabolomics operates by combining the data-dependent precursor/product ion discovery of High-Resolution Mass Spectrometry (HRMS, e.g., Waters XEVO G2-XS QTOF) with the quantitative precision of Triple Quadrupole linear ion trap MS (e.g., QTRAP 6500+). By building a customized, high-density MRM channel library directly from representative biological pool samples, researchers gain the breadth of non-targeted analysis alongside the quantitative strength of targeted assays.
| Metabolomic Strategy | Ion Selection Method | Quantitative Performance | Dynamic Range | Large Cohort Suitability |
|---|---|---|---|---|
| Untargeted (Full Scan / HRMS) | Data Dependent Acquisition (DDA / DIA) | Semi-quantitative (Peak Area Signal) | 103 - 104 | Moderate (High Batch Effect Sensitivity) |
| Targeted (Standard MRM) | Pre-defined Pure Chemical Standards | Absolute Quantitation (μM, ng/mL) | 105 - 106 | High (Limited to <100 compounds) |
| BMKGene Pseudotargeted (MRM-HP) | Automated High-Density Pool MRM Library | High-Precision Relative / Semi-Absolute | 105 - 106 | Superior (Thousands of Metabolites) |
Industrial Landscape, Market Trends & Translational Application
The global metabolomics market is experiencing rapid expansion, driven by clinical biomarker discovery, agricultural phenomics, and biopharmaceutical R&D. Industry leaders and premier research institutes are moving toward integrated workflows that combine genomics, spatial transcriptomics, and high-throughput metabolomics to decipher complex biological pathways.
1. Precision Medicine & Biomarker Discovery
In clinical research, identifying reproducible metabolic signatures for early disease detection (e.g., oncology, metabolic syndromes, neurodegenerative disorders) demands wide sample cohorts. Pseudotargeted metabolomics suppresses background noise, delivering reproducible biomarkers suitable for CLIA/CAP translational validation.
2. Crop Breeding & Agricultural Trait Mapping
Academic partnerships in plant sciences leverage pseudotargeted metabolite GWAS (mGWAS) to correlate specific metabolic pathways (e.g., flavonoids, alkaloids, lipids) with stress tolerance, yield, and crop quality, significantly accelerating molecular breeding cycles.
3. Microbiome & Host Metabolite Interactions
Understanding gut microbiota function requires quantitative profiling of short-chain fatty acids (SCFAs), bile acid pools, and microbial secondary metabolites. Our automated pipelines enable multi-omic integration between metagenomic sequencing and metabolic output.
Biomarker Technologies (BMKGene) Corporate Strength
Biomarker Technologies (BMKGene), founded in 2009, is a leading genomics service provider with over 16 years of continuous innovation in high-throughput sequencing and bioinformatics. Backed by more than 60 national invention patents and 200+ software copyrights, we deliver comprehensive multi-omics solutions—spanning genomics, metagenomics, epigenetics, single-cell omics, transcriptomics, and our proprietary BMKMANU S3000 spatial transcriptome technology—supported by our advanced BMKCloud bioinformatics platform. We have established long-term collaborations with organizations across 84 regions worldwide, providing reliable genomic solutions on a global scale.
Leading Multi-Level High-Throughput Platforms
Advanced Sequencing & Mass Spec Platforms
PacBio platforms: Sequel II, Sequel, RSII
Nanopore platforms: PromethION P48, GridION X5 MinION
10X Genomics: 10X ChromiumX, 10X Chromium Controller
Illumina platforms: NovaSeq
BGI-sequencing platforms: DNBSEQ-G400, DNBSEQ-T7
Bionano Irys system
Waters XEVO G2-XS QTOF
QTRAP 6500+
Over 20,000 Square Feet Facility
• Advanced biomolecular laboratory instruments.
• Standardized labs for sample extraction, library construction, clean rooms, and mass spectrometry/sequencing suites.
• Rigorous SOP compliance guaranteeing exceptional reproducibility from sample prep to data output.
BMKCloud Bioinformatics Platform
• Self-developed BMKCloud interactive bioinformatic platform.
• CPUs with 41,104 memory and 3 PB total storage capacity.
• 4,260 computing cores delivering peak processing power exceeding 121,708.8 Gflop/s.
Fully Automated Platform for Next-Generation Processing: Brilliant Lab 1000
Biomarker Technologies (BMKGene) and PerkinElmer have jointly built a fully automated experimental production line, called Brilliant Lab 1000 (BL1000), applied to high-throughput library construction and automated sample processing.
BMKGene strives to continuously elevate our entire spectrum of multi-omics services in terms of throughput, turnaround times, and quantitative precision, providing academic partners worldwide with top-tier analytical services.
State-of-the-Art Laboratory Facilities
Enterprise Qualifications, ISO Certifications & Patents
Our persistent commitment to research excellence is demonstrated by rigorous ISO quality management systems and extensive intellectual property portfolios.
Academic Cooperation Workflow & Support Model
We provide end-to-end support for university labs, medical institutes, and government research centers—from experimental design to high-impact publication support.
Consultation & Study Design
In-depth alignment on research hypotheses, sample size calculations, matrix selection, and customized MRM ion-pair target strategy.
Sample Prep & LC-MS Profiling
Standardized extraction under strict SOPs, followed by Waters QTOF screening and QTRAP 6500+ pseudotargeted quantitative acquisition.
Multi-Omics Bio-Data Mining
Integrated pipeline processing via BMKCloud, correlation analysis with transcriptomics/genomics data, pathway enrichment, and mGWAS mapping.
Co-Author & Publishing Support
Comprehensive bioinformatics reports, publication-ready figures, raw data repository submissions, and expert methodological writeups.
Initiate an Academic Collaboration Project Today
For inquiries regarding custom pseudotargeted panels, joint grant applications, or volume pricelists, please connect with our team. We guarantee a response within 24 hours.
Inquire Now / Submit Project Details
Small RNA Sequencing-Illumina
De novo Bacterial Genome Assembly
Genome-wide Association Analysis
Metagenomic Sequencing -NGS
