16S / 18S / ITS Ikurikirana rya Amplicon-PacBio
Inyungu za serivisi
ØGusoma birebire byerekana uburebure bwuzuye bwa 16S / 18S / ITS
ØByukuri byukuri guhamagarwa hamwe na PacBio CCS uburyo bukurikirana
ØUbwoko-urwego rwo gukemura muri OTU / ASV itangazo
ØQIIME2 iheruka gusesengura imigendekere hamwe nisesengura ritandukanye mubijyanye na base, annotation, OTU / ASV.
ØBirakoreshwa mubyiciro bitandukanye bya mikorobe
ØBMK ifite uburambe bunini hamwe nicyitegererezo kirenga 100.000 / umwaka ， gitwikira ubutaka, amazi, gaze, isuka, umwanda, amara, uruhu, umuyonga wa fermentation, udukoko, ibimera, nibindi.
ØBMKCloud yorohereje gusobanura amakuru arimo ibikoresho 45 byo gusesengura byihariye
Ibisobanuro bya serivisi
| UrukurikiraneIhuriro | Isomero | Basabwe gutanga amakuru | Bigereranijwe guhindukira |
| Urutonde rwa PacBio II | SMRT-inzogera | 5K / 10K / 30K CCS | Iminsi 30 |
Isesengura rya bioinformatics
üKugenzura ubuziranenge bwamakuru
üIhuriro rya OTU / De-urusaku (ASV)
üIbisobanuro bya OTU
üAlpha itandukanye
üUbwoko bwa Beta
üIsesengura hagati yitsinda
üIsesengura ryishyirahamwe rirwanya ibintu byubushakashatsi
üImikorere ya gene
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
KuriIbikuramo ADN:
| Ubwoko bw'icyitegererezo | Umubare | Kwibanda | Isuku |
| Ibikuramo ADN | ＞ 1 μg | Ng 20 ng / μl | OD260 / 280 = 1.6-2.5 |
Kubidukikije:
| Ubwoko bw'icyitegererezo | Basabwe uburyo bwo gutoranya |
| Ubutaka | Umubare w'icyitegererezo: hafi.5 g;Ibintu bisigaye byumye bigomba gukurwa hejuru;Gusya ibice binini hanyuma unyure muri mm 2 muyungurura;Icyitegererezo cya Aliquot muri sterile EP-tube cyangwa cyrotube yo kubika. |
| Umwanda | Umubare w'icyitegererezo: hafi.5 g;Kusanya hamwe na aliquot ntangarugero muri sterile EP-tube cyangwa cryotube kugirango ubike. |
| Amara | Ingero zigomba gutunganywa mugihe cya aseptic.Koza imyenda yakusanyirijwe hamwe na PBS;Centrifuge PBS hanyuma ukusanyirize imvura muri EP-tubes. |
| Umuyoboro | Umubare w'icyitegererezo: hafi.5 g;Kusanya hamwe na aliquot sludge sample muri sterile EP-tube cyangwa cryotube kugirango ubike |
| Amazi | Kurugero hamwe na mikorobe nkeya, nkamazi ya robine, amazi meza, nibindi, Kusanya byibuze amazi 1 L hanyuma unyure muri 0.22 μm muyungurura kugirango ukungahaye mikorobe kuri membrane.Bika ururenda muri sterile. |
| Uruhu | Witonze witonze hejuru yuruhu ukoresheje ipamba ya sterile cyangwa urubingo rwo kubaga hanyuma ubishyire mubitereko bya sterile. |
Basabwe Gutanga Icyitegererezo
Hagarika icyitegererezo muri azote yuzuye mumasaha 3-4 hanyuma ubike muri azote yuzuye cyangwa -80 kugirango ubike igihe kirekire.Icyitegererezo cyoherezwa hamwe na ice-ice irakenewe.
Akazi ka serivisi
Icyitegererezo
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
1.Ibipimo byerekana kuri V3 + V4 (Illumina) -bishingiye kuri mikorobe yumuryango hamwe nuburebure bwuzuye (PacBio) -bishingiye kumurongo.
(Amakuru yimishinga 30 yatoranijwe yakoreshejwe kubarurishamibare)
2.Ibipimo byerekana ibisobanuro byuzuye bya amplicon ikurikiranye kurwego rwubwoko butandukanye
3.Gukwirakwiza ubwoko
4.Igiti cya phylogeneque
Urubanza rwa BMK
Guhura kwa Arsenic bitera inzitizi zo munda kwangirika hamwe nigikorwa cyo gukora igifu-umwijima biganisha ku gutwika na pyroptose yumwijima mu njangwe.
Byatangajwe:Ubumenyi bwibidukikije byose,2021
Ingamba zikurikirana:
Icyitegererezo: Igenzura vs 8 mg / kg ATO itsinda ryerekanwe
Gukurikirana amakuru yatanzwe: 102,583 mbisi ya CCS ikurikirana muri rusange
Igenzura: 54,518 ± 747 CCS ikora neza
ATO-yashyizwe ahagaragara: 45,050 ± 1675 CCS ikora neza
Ibisubizo by'ingenzi
Alpha itandukanye:Kugaragara kwa ATO byahinduye cyane mikorobe yo munda ubukire nubwinshi mubisimba.
Isesengura ry'imiterere:
Kurwego rwa phylum: phyla 2 ya bagiteri yagaragaye gusa mumatsinda yo kugenzura
Urwego rwubwoko: genera 6 wasangaga zitandukanye cyane mubwinshi ugereranije
Urwego rwubwoko: Ubwoko 36 bwamenyekanye muri rusange, 6 muri bwo butandukanye cyane mubwinshi
Reba
Thingholm, LB, n'abandi.“Abantu bafite umubyibuho ukabije bafite Diyabete yo mu bwoko bwa 2 kandi badafite Ububiko butandukanye bwa mikorobe ikora neza hamwe n'imiterere.”Akagari ka Cell & Microbe26.2 (2019).
