Ubushinwa Fungal Genome uruganda nababikora |BMK

Fungal Genome

Ikoranabuhanga rya Biomarker ritanga ubushakashatsi bwa genome, genome nziza na gen-yuzuye ya fungal bitewe nintego yihariye yubushakashatsi.Ikurikiranyabihe rya genome, guterana hamwe no gutangaza imikorere birashobora kugerwaho muguhuza ibisekuruza bizakurikiraho + Urwego rwa gatatu rukurikirana kugirango tugere kumurongo wo murwego rwohejuru.Tekinoroji ya Hi-C irashobora kandi gukoreshwa kugirango byorohereze inteko ya chromosome.

Ihuriro ：Urutonde rwa PacBio II

Nanopore Isezerano P48

Illumina NovaSeq 6000

Twandikire

Ibisobanuro bya serivisi

Ibisubizo bya Demo

Inyungu za serivisi

ØIngamba nyinshi zikurikirana ziboneka kumigambi itandukanye yubushakashatsi

ØInararibonye cyane muri bacteri genome ikorana na genome zirenga 10,000.

ØUmwuga nyuma yo kugurisha itsinda ryunganira tekinike ryujuje ibyifuzo byubushakashatsi bwihariye.

Ibisobanuro bya serivisi

Serivisi	Ingamba zikurikirana	Ubwiza bufite ireme	Bigereranijwe guhindukira
Ikarita nziza	Illumina 50X + Nanopore 100X	Contig N50≥2 Mb	Iminsi 35 y'akazi
Ikarita nziza	PacBio HiFi 30X	Contig N50≥2 Mb	Iminsi 35 y'akazi
Ikarita yuzuye	Illumina 50X + Nanopore 100X (Pacbio HiFi 30X) + Hi-C 100X	Ikigereranyo cya Chromosome> 90%	Iminsi 45 y'akazi

Isesengura rya bioinformatics

üKugenzura ubuziranenge bwamakuru

üInteko rusange

üIsesengura ryibigize genome

üImikorere ya gen

üKugereranya genomic igereranya

Icyitegererezo gisabwa no gutanga

Icyitegererezo gisabwa:

KuriIbikuramo ADN:

Ubwoko bw'icyitegererezo	Umubare	Kwibanda	Isuku
Ibikuramo ADN	Μ 3 μg	Ng 20 ng / μl	OD260 / 280 = 1.6-2.5

Ku byitegererezo by'inyama:

Ubwoko bw'icyitegererezo

Basabwe kuvura icyitegererezo

Icyitegererezo cyo kubika no kohereza

Fungus idasanzwe

Itegereze umusemburo munsi ya microscope hanyuma ukusanyirize mugice cyazo

Hindura umuco (urimo selile zigera kuri 3-4.5e9) muri eppendorf ya 1.5 cyangwa 2.(Komeza ku rubura)

Centrifuge umuyoboro muminota 1 kuri 14000 g kugirango ukusanye bagiteri kandi ukureho supernatant witonze

Funga umuyoboro hanyuma uhagarike bagiteri muri azote yuzuye byibuze 30 min.Bika umuyoboro muri -80 frigo.

Hagarika icyitegererezo muri azote yuzuye mumasaha 3-4 hanyuma ubike muri azote yuzuye cyangwa -80 kugirango ubike igihe kirekire.Icyitegererezo cyoherezwa hamwe na ice-ice irakenewe.

Macro Fungus

Tissue mugice gikura cyane birasabwa.

Kwoza tissue hamwe n'amazi adafite endotoxine, hanyuma 70% ya ethonal.

Bika icyitegererezo muri cryo-tubes.