Muraho-C ishingiye ku Nteko rusange
Inyungu za serivisi
Incamake ya Hi-C
(Lieberman-Aiden E n'abandi.,Ubumenyi, 2009)
ØNtibikenewe mu kubaka abaturage ba genetike ya contig anchoring
ØUbucucike buri hejuru buganisha kumurongo wo hejuru hejuru ya 90% ；
ØGushoboza gusuzuma no gukosora inteko zihari;
ØIgihe gito cyo guhindukirana hamwe nukuri neza murwego rwa genome;
ØUbunararibonye bwinshi hamwe nibitabo birenga 1000 Hi-C byubatswe kumoko arenga 500;
ØImanza zirenga 100 zatsinzwe hamwe ningaruka zirenga 760;
ØHi-C ishingiye kuri genome ya genome ya polyploide, igipimo cya ankoring 100% cyagezweho mumushinga wabanjirije.
ØMu nzu patenti hamwe nuburenganzira bwa software kubushakashatsi bwa Hi-C no gusesengura amakuru
ØKwikorera wenyine kwifashisha amashusho yerekana amakuru, ashoboza guhagarika intoki kugenda, gusubira inyuma, gukuraho no gusubiramo.
Ibisobanuro bya serivisi
| UrukurikiraneIhuriro | Isomero | Ubujyakuzimu busabwa | Bigereranijwe guhindukira | Inteko |
| Illumina | 300-350 bp | Genome yoroshye ≥ 100 × Genome igoye ≥ 150 × | Amezi 3 (Ukurikije amoko) | Ikigereranyo cya Anchoring ≥ 90% (Ukurikije amoko) |
Isesengura rya bioinformatics
üKugenzura ubuziranenge bwamakuru
üHi-C isomero ryiza
üMuraho-C ishingiye kuri genome
üIsuzuma nyuma yinteko
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
Ku nyamaswa:
1.Umusaya (Ubukonje): ≥ 2 g;
2.Amaraso yuzuye (Ntabwo akonje): ml 2 ml (kubitabo 3)
Ku bimera:
1.Imbuto (urugero ingemwe zikiri nto zihingwa mu mbuto, ingemwe z'umuco wa tissue, nibindi): gukusanya amababi yose hamwe nigiti gito;
2.Ibihingwa bikuze: gukusanya uruti rwibiti hamwe namababi mashya 1 cyangwa 2 yambere yegereye uruti.
* Amafaranga asabwa kuri buri sample ≥ 4 g.(Imikoreshereze yuburyo bwo kubaka isomero ni 1 g kuri buri somero).
Basabwe Gutanga Icyitegererezo
Ibikoresho: ml 2 ya centrifuge (Tin foil ntabwo isabwa)
Kubenshi mubitegererezo, turasaba kutabika muri Ethanol.
Icyitegererezo cyerekana: Icyitegererezo kigomba kuba cyanditseho neza kandi gihuye nicyitegererezo cyatanzwe.
Kohereza: Kuma-ice: Ingero zigomba kubanza gupakirwa mumifuka hanyuma zigashyingurwa mu rubura.
Akazi ka serivisi
Igishushanyo mbonera
Icyitegererezo
Ikigeragezo cya Hi-C
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
*Ibisubizo byerekanwa hano byose biva muri genomes yatangajwe na Biomarker Technologies
1.Hi-C ikarita yubushyuhe bwaKamptotheca acuminatagenome.Nkuko bigaragara ku ikarita, ubukana bwimikoranire bufitanye isano nintera yumurongo, byerekana inteko ya chromosome-yuzuye.(Ikigereranyo cya Anchoring: 96.03%)
Kang M n'abandi.,Itumanaho rya Kamere, 2021
2.Hi-C yorohereje kwemeza ihinduka hagatiGossypium hirsutumL. TM-1 A06 naG. arboreumChr06
Yang Z n'abandi.,Itumanaho rya Kamere, 2019
3. Guteranya no gutandukanya biallelic genome yimyumbati SC205.Hi-C ubushyuhe bwerekanwe gutandukana neza muri chromosomes.
Hu W n'abandi.,Igihingwa cya molekile, 2021
4.Hi-C ubushyuhe bwubwoko bubiri bwa Ficus:F.microcarpa(igipimo cya ankoring: 99.3%) naF.hispida (igipimo cya ankoring: 99.7%)
Zhang X n'abandi.,Akagari, 2020
Urubanza rwa BMK
Genome yigiti cyitwa banyan hamwe na pollinator wasp itanga ubushishozi kubijyanye na fig-wasp coevolution
Byatangajwe: Akagari, 2020
Ingamba zikurikirana:
F. microcarpagenome: Yegeranye.84 X PacBio RSII (36.87 Gb) + Hi-C (44 Gb)
F. hispidagenome: Yegeranye.97 X PacBio RSII (36.12 Gb) + Hi-C (60 Gb)
Eupristina verticillatagenome: Yegeranye.170 X PacBio RSII (65 Gb)
Ibisubizo by'ingenzi
1.Ibiti bibiri bya banyan hamwe na polinator imwe wasp genome yubatswe hakoreshejwe urutonde rwa PacBio, Hi-C hamwe n'ikarita yo guhuza.
(1)F. microcarpagenome: Hashyizweho inteko ya 426 Mb (97.7% yubunini bwa genome igereranijwe) yashizweho hamwe na N50 ya 908 Kb, amanota ya BUSCO ya 95,6%.Muri rusange, 423 Mb zikurikirana zometse kuri chromosomes 13 na Hi-C.Ibisobanuro bya genome byatanze genes 29.416.
(2)F. Hispidagenome: Inteko ya 360 Mb (97.3% yubunini bwa genome igereranijwe) yatanze umusaruro hamwe na N50 ya 492 Kb na BUSCO amanota 97.4%.Urutonde rwa 359 Mb rwashyizwe kuri 14 chromosomesby Hi-C kandi birasa cyane n'ikarita ihuza cyane.
(3)Eupristina verticillatagenome: Hashyizweho inteko ya 387 Mb (Bigereranijwe ubunini bwa genome: 382 Mb) hamwe na contig N50 ya 3.1 Mb na BUSCO amanota 97.7%.
2.Gereranya genomics igereranya yerekanye umubare munini wimiterere itandukanyeFicusgenome, yatangaga umutungo ntangarugero kubushakashatsi bwihindagurika.Ubu bushakashatsi, kunshuro yambere, bwatanze ubushishozi kuri fig-wasp coevolution kurwego rwa genomic.
 Igishushanyo cya sikos kuri genomic ibiranga bibiriFicusgenome, harimo chromosomes, kwigana ibice (SDs), transposons (LTR, TEs, ADN TEs), imvugo ya gene na synteny |  Kumenyekanisha Y chromosome na kugena igitsina umukandida gene |
Zhang, X., n'abandi.“Genome z'igiti cya Banyan na Pollinator Wasp zitanga ubushishozi kuri Coevolution.”Akagari 183.4 (2020).
