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Urutonde rwa Metagenomic (NGS)
Metagenome bivuga icyegeranyo cyibintu byose bikomoka kumoko avanze yibinyabuzima, nka metagenome yibidukikije, metagenome yabantu, nibindi.Ikurikiranwa rya Metagenomic nigikoresho cya molekuline gikoreshwa mu gusesengura ibikoresho bivangwa na genomique byakuwe mu ngero z’ibidukikije, bitanga amakuru arambuye ku moko atandukanye no ku bwinshi, imiterere y’abaturage, isano ya phylogeneque, ingirabuzimafatizo ikora hamwe n’ibidukikije.
Ihuriro ：Illumina NovaSeq6000
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Gukurikirana Metagenomic-Nanopore
Metagenomics nigikoresho cya molekuline gikoreshwa mu gusesengura ibikoresho bivangwa na genomic bivanwa mu ngero z’ibidukikije, bitanga amakuru arambuye mu miterere y’ibinyabuzima no ku bwinshi, imiterere y’abaturage, isano ya phylogeneque, ingirabuzimafatizo ikora hamwe n’ibidukikije, n'ibindi. Kuri metagenomic.Imikorere yayo idasanzwe mubisomwa birebire byongerewe imbaraga zo gusesengura metagenomic, cyane cyane inteko ya metagenome.Ufashe ibyiza byo gusoma-uburebure, Nanopore ishingiye kuri metagenomic ubushakashatsi irashobora kugera ku nteko ikomeza ugereranije na metagenomics.Byashyizwe ahagaragara ko metagenomics ishingiye kuri Nanopore yabyaye genoside yuzuye kandi ifunze mikorobe (Moss, EL, et al.,Kamere ya Biotech, 2020)
Ihuriro ：Nanopore Isezerano P48
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16S / 18S / ITS Ikurikirana rya Amplicon-PacBio
Subunit kuri 16S na 18S rRNA ikubiyemo uturere twabitswe cyane hamwe na hyper-variable ni urutoki rwiza rwa molekuline kugirango tumenye ibinyabuzima bya prokaryotic na eukaryotic.Ukoresheje uburyo bukurikirana, izi amplicons zirashobora kwibasirwa hashingiwe kubice byabitswe kandi uturere twa hyper-variable turashobora kurangwa byuzuye kubiranga mikorobe bigira uruhare mubushakashatsi bukubiyemo isesengura ryimiterere ya mikorobe, tagisi, phylogene, nibindi. ) Urutonde rwa PacBio rushobora kubona ibisobanuro birebire bisomwa neza, bishobora gutwikira amplicons yuzuye (hafi 1.5 Kb).Kugura kwagutse k'umurima wa genetike byongereye cyane imiterere yo gutangaza amoko muri bagiteri cyangwa mugace ka fungi.
Ihuriro ：Urutonde rwa PacBio II
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16S / 18S / ITS Ikurikirana rya Amplicon-NGS
16S / 18S / ITS ikurikirana ya amplicon igamije kwerekana phylogene, tagisi, nubwinshi bwamoko mumuryango wa mikorobe ukoresheje iperereza ryibicuruzwa bya PCR byerekana ibimenyetso bikomoka kumurugo birimo ibice byaganiriweho cyane kandi birenze urugero.Kwinjiza kwintoki za molekuline nziza na Woeses et al, (1977) iha imbaraga microbiome yerekana umwirondoro.Urukurikirane rwa 16S (bagiteri), 18S (fungi) hamwe na spacer yimbere yimbere (ITS, fungi) ituma hamenyekana amoko menshi kimwe nubwoko budasanzwe kandi butamenyekana.Iri koranabuhanga ryahindutse igikoresho kinini kandi gikomeye muguhitamo mikorobe itandukanye mubidukikije, nkumunwa wabantu, amara, umwanda, nibindi.
Ihuriro ：Illumina NovaSeq6000
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Indwara ya bagiteri na fungal Yongeye gukurikiranwa
Indwara ya bagiteri na fungal yongeye gukurikiranwa nigikoresho gikomeye cyo kurangiza genome za bagiteri zizwi na fungi, ndetse no kugereranya genome nyinshi cyangwa gushushanya genome yibinyabuzima bishya.Ni ngombwa cyane gutondekanya genome zose za bagiteri na fungi kugirango tubyare genomisiyo nyayo, gukora mikorobe hamwe nubundi bushakashatsi bugereranya.
Ihuriro: Illumina NovaSeq 6000
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Fungal Genome
Ikoranabuhanga rya Biomarker ritanga ubushakashatsi bwa genome, genome nziza na gen-yuzuye ya fungal bitewe nintego yihariye yubushakashatsi.Ikurikiranyabihe rya genome, guterana hamwe no gutangaza imikorere birashobora kugerwaho muguhuza ibisekuruza bizakurikiraho + Urwego rwa gatatu rukurikirana kugirango tugere kurwego rwo hejuru.Tekinoroji ya Hi-C irashobora kandi gukoreshwa kugirango byorohereze inteko kurwego rwa chromosome.
Ihuriro ：Urutonde rwa PacBio II
Nanopore Isezerano P48
Illumina NovaSeq 6000
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Indwara ya bagiteri yuzuye
Biomarker Technologies itanga serivise ikurikirana mugukora genome yuzuye ya bagiteri ifite icyuho cya zeru.Ibikorwa nyamukuru bya bagiteri byuzuye byubaka genome bikubiyemo ibisekuruza bya gatatu bikurikirana, guteranya, gutangaza imikorere hamwe nisesengura rya bioinformatic ryujuje intego zubushakashatsi.Ibisobanuro birambuye kuri genoside ya bagiteri itanga imbaraga zerekana uburyo bwibanze bushingiye ku binyabuzima, ibyo bikaba byanatanga ibisobanuro bifatika kubushakashatsi bwa genomique mu bwoko bwa eukaryotic.
Ihuriro ：Nanopore Isezerano P48 + Illumina NovaSeq 6000
Urutonde rwa PacBio II
