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Ibicuruzwa

Kugabanya Guhagararirwa Bisulfite Urukurikirane (RRBS)

Reduced Representation Bisulfite Sequencing (RRBS) Featured Image
  • Reduced Representation Bisulfite Sequencing (RRBS)

Ubushakashatsi bwa methylation ya ADN buri gihe bwabaye ingingo ishyushye mubushakashatsi bwindwara, kandi bufitanye isano rya bugufi nimvugo ya gene hamwe na pheno-typic.RRBS nuburyo nyabwo, bukora neza kandi bwubukungu kubushakashatsi bwa methylation ya ADN.Gutunganya uturere twa CpG hamwe na CpG mukarere ka enzymatique (Msp I), hamwe na Bisulfite ikurikirana, bitanga ibisubizo bihanitse bya ADN methylation.

Ihuriro: Illumina NovaSeq 6000


Ibisobanuro bya serivisi

Ibisobanuro bya serivisi

Ihuriro: NovaSeq 6000 platform, PE150

Ubwoko bw'isomero: 200-400bp shyiramo bisulfite ivura isomero rya ADN

Ingamba zikurikirana: Byombi-Impera 150 bp

Saba ibyasohotse: 10 Gb amakuru yibanze / icyitegererezo

Icyitegererezo

Ingano ya ADN: ≥ 2ug

Kwibanda kuri ADN: ≥ 20ng / μl.

Isuku: OD260 / 280 = 1.8 kugeza 2.0 nta kwangirika cyangwa kwanduza RNA

Akazi ka serivisi

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Icyitegererezo

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Kubaka isomero

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Urukurikirane

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Isesengura ryamakuru

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