Urutonde ruto rwa RNA-Illumina
Inyungu za serivisi
Øishingiro ryambere kuri 5 'iherezo rifite icyifuzo gikomeye kuri U kugirango ubone RNAs
ØmiRNA intego yo guhanura gene
ØAmakuru akoreshwa mugukora isesengura hamwe na mRNA
ØmiRNA imvugo itandukanye
ØmiRNA intego yo guhanura gene
ØBMKCloud ishingiye kubisubizo: Gutanga amakuru yihariye kubucukuzi buboneka kurubuga.
ØSerivise nyuma yo kugurisha ifite agaciro kumezi 3 iyo umushinga urangiye
Icyitegererezo gisabwa no gutanga
Icyitegererezo gisabwa:
Nucleotide:
| Isuku | Ubunyangamugayo | Kwanduza | Umubare |
| OD260 / 280≥1.7-2.5 ； OD260 / 230≥0.5-2.5 ； | Kubimera: RIN≥6.5;Ku nyamaswa: RIN≥7;28S / 18S≥1.0;bigarukira cyangwa bidafite ishingiro | Ntarengwa cyangwa ntayo poroteyine cyangwa ADN yanduye kuri gel. | Umwanzuro.≥300 ng / μl;Inkingi ≥ 10 μl;Igiteranyo ≥ 3 μg |
Tissue: Uburemere (bwumye): ≥1 g
* Kuri tissue ntoya ya mg 5, turasaba kohereza flash ya flash ikonje (muri azote yuzuye).
Guhagarika ingirabuzimafatizo: Kubara Akagari = 3 × 107
* Turasaba kohereza lysate ya selile ikonje.Mugihe iyo selile ibara ntoya 5 × 105, flash yahagaritswe muri azote yuzuye.
Ingero zamaraso:
PA × geneBloRNRNube;
6mLTRIzol n'amaraso 2mL (TRIzol: Amaraso = 3: 1)
Basabwe Gutanga Icyitegererezo
Ibirimwo:
2 ml centrifuge tube (Tin foil ntabwo isabwa)
Icyitegererezo cyerekana: Itsinda + kwigana urugero A1, A2, A3;B1, B2, B3 ... ...
Kohereza:
1.Ibara ryumye: Ingero zigomba gupakirwa mumifuka hanyuma zigashyingurwa mu rubura.
2.Imiyoboro ihamye: Ingero za RNA zirashobora gukama mumashanyarazi ya RNA (urugero RNAstable®) hanyuma ikoherezwa mubushyuhe bwicyumba.
Akazi ka serivisi
Igishushanyo mbonera
Icyitegererezo
Gukuramo RNA
Kubaka isomero
Urukurikirane
Isesengura ryamakuru
Serivisi nyuma yo kugurisha
Bioinformatics
 |
1.miRNA Kumenyekanisha
Buri mukandida abanziriza udushya miRNAs yahanuwe na miRDeep2 afite ishusho ya pdf yerekana imiterere nuburebure bwikurikiranya.
miRNA ibanziriza imiterere nuburebure bwikurikiranya
2.KEGG Gutunganyiriza DE-miRNA Intego
Muri iki cyiciro, Biomarker yabanje gusuzuma niba inzira zirenze urugero hamwe na DE-miRNA igenewe gen.Ibintu bikungahaye hamwe nigeragezwa ryabarobyi byakoreshejwe mukugena urwego rwo gukungahaza n'akamaro k'inzira.Ikigereranyo cyo kubara ubutunzi kirerekanwa hepfo.
KEGG itunganyiriza inzira kuri DE-miRNA igenewe gen
3.GO Isesengura Ryiza kuri DE-miRNA Intego Zigenewe
clusterProfiler yakoreshejwe mubisesengura rya GO kuri DE-miRNA gen.Igishushanyo mbonera cya acyclicike yamagambo akungahaye yakozwe kugirango yerekane imiterere yamagambo.Mu gishushanyo, icyerekezo cyimyambi kigaragaza isano iri hagati yamagambo, ni ukuvuga imitwe irenze umwihariko wabo.Igishushanyo cya acylic cyerekanwe na DE-miRNA genes yerekanwe kumashusho hepfo.
TopGO yayoboye igishushanyo cya acyclic ya DE-miRNA igenewe gen
Urubanza rwa BMK
Isesengura ryuzuye rya MiRNA na Gen bifitanye isano nubwiza bwinyama byerekana ko Gga-MiR-140-5p igira ingaruka kumyuka yibinure mu nkoko
Byatangajwe:Imiterere ya selile na Biochemie ，2018
Ibisubizo by'ingenzi
Inkoko zo gutinda-igihe cyerekanaga urwego rwo hasi rwerekana imiterere ya miRNAs kuruta inkoko zabana.
Urusobekerane rwa mirNA-mrna rushobora kugira ingaruka kumiterere yinyama zarubatswe
gga-miR-140-5p iteza imbere itandukaniro rya adipocyte mukugenzura RXRG.
 gga-miR-140-5p iteza imbere inkoko intramuscular preadipocyte itandukanya 3'UTR ya RXRG |
Reba
Zhang M, Li DH, Li F, n'abandi.Isesengura ryuzuye rya MiRNA na Gen bifitanye isano nubwiza bwinyama byerekana ko Gga-MiR-140-5p igira ingaruka kumyuka yibinure mu nkoko [J].Imiterere ya selile na Biochemie, 2018: 2421-2433.DOI ： 10.1159 / 000489649
