Oloa

10x Genomics Visium Spatial Transcriptome

Spatial transcriptomics ose tekonolosi fa'aonaponei e mafai ai e tagata su'esu'e ona su'esu'e fa'ata'ita'iga fa'aaliga o kenera i totonu o masini a'o fa'asaoina a latou fa'afanua fa'afanua.O le tasi tulaga mamana i lenei vaega o le 10x Genomics Visium faʻatasi ma le faʻasologa o le Illumina.O le mataupu faavae o le 10X Visium o loʻo taoto i luga o se vaʻa faʻapitoa faʻatasi ai ma se nofoaga e puʻeina e tuʻuina ai vaega o mea.O le vaega lea e pu'e ai o lo'o iai ni fa'ailoga fa'ailoga, e tutusa uma ma se nofoaga fa'apitoa fa'apitoa i totonu ole aano.O mole mole RNA na pu'eina mai le aano o lo'o fa'ailogaina i fa'amatalaga mole mole tulaga ese (UMIs) i le taimi o le fa'aliliuga fa'aliliuga.O nei fa'ailoga fa'ailoga ma UMI e mafai ai ona fa'ata'atia ma'oti fa'afanua ma fa'avasegaina o fa'amatalaga o kenera i se fa'ai'uga e tasi le sela.O le tu'ufa'atasiga o fa'ata'ita'iga fa'ailoga va'ava'ai ma UMI e fa'amautinoa ai le sa'o ma le fa'apitoa o fa'amaumauga na gaosia.I le faʻaaogaina o lenei Spatial Transcriptomics technology, e mafai ai e tagata suʻesuʻe ona maua se malamalama loloto atu i le faʻalapotopotoga faʻapitoa o sela ma fesoʻotaʻiga mole mole lavelave o loʻo tutupu i totonu o masini, e ofoina atu ai faʻamatalaga taua i le faʻaogaina o faiga faʻavae i le tele o vaega, e aofia ai le oncology, neuroscience, developmental biology, immunology. , ma su'esu'ega fa'ato'aga.

Fa'avae: 10X Genomics Visium ma Illumina NovaSeq

FOB tau:US $0.5 - 9,999 / Vaega

Min. Order Quantity:100 Vaega/Pisi

Avanoa Avanoa:10000 Piece/Pieces i le masina

Faafesootai matou

Au'aunaga Fa'amatalaga

Bioinformatics

I'uga Fa'aaliga

Lomiga fa'apitoa

Vaega

● I'uga: 100 µM

● Vaitafe Va'ai: 55 µM

● Numera o togi: 4992

● Pu'e vaega: 6.5 x 6.5 mm

● O nofoaga taʻitasi taʻitasi o loʻo faʻapipiʻiina i mea muamua e aofia ai vaega e 4:

- poly(dT) si'usi'u mo mRNA priming ma cDNA synthesis

- Tulaga Fa'ailoga Molecular (UMI) e fa'asa'o ai le fa'aituau fa'alautele

- Fa'ailoga fa'avanoa

- Fa'asologa fa'asologa o le faitau vaega 1 fa'asologa fa'asologa

● H&E pisia o vaega

Tulaga lelei

●Au'aunaga tutasi: tu'ufa'atasia uma le poto masani ma laasaga fa'avae, e aofia ai le cryo-sectioning, staining, tissue optimization, spatial barcoding, saunia faletusi, sequencing ma bioinformatics .

● Au faʻapitoa faʻapitoa: ma le poto masani i le silia ma le 250 ituaiga o mea'ai ma le 100+ ituaiga e aofia ai tagata, isumu, mamame, i'a ma laau.

●Fa'afouga taimi moni i le galuega atoa: ma le pulea atoatoa o le alualu i luma faataitai.

●Bioinformatics masani:afifi e aofia ai 29 auiliiliga ma 100 + maualuga-lelei fuainumera.

●Fa'avasegaina fa'amatalaga fa'amatalaga ma fa'aaliga: avanoa mo talosaga su'esu'e eseese.

●Su'esu'ega fa'atasi fa'atasi ma le fa'asologa o le mRNA e tasi

Fa'amatalaga

Fa'ata'ita'iga Manaoga

Faletusi

Fuafuaga fa'asologa

Fa'amatalaga fautuaina

Puleaina lelei

Fa'ata'ita'iga cryo fa'apipi'i OCT, fa'ata'ita'iga FFPE

(Tamita sili: pe tusa 6x6x6 mm3)

3 poloka ile fa'ata'ita'iga

10X Visium cDNA faletusi

Illumina PE150

50K PE faitau i le nofoaga

(60Gb)

RIN>7

Mo nisi fa'amatalaga i fa'ata'ita'iga tapenaga ta'iala ma au'aunaga, fa'amolemole lagona le saoloto e talanoa i aBMKGENE tagata poto

Au'aunaga Galuega

I le fa'ata'ita'iga la'asaga o tapenaga, e faia se fa'ata'ita'iga muamua o le RNA fa'atosina ina ia mautinoa e mafai ona maua se RNA maualuga.I le tulaga o le su'esu'eina o mea'ai o lo'o pisipisia ma va'ava'ai ai vaega ma fa'amanino tulaga mo le fa'asa'olotoina o le mRNA mai le tino.O le fa'atonuga fa'apitoa e fa'aaogaina i le taimi o le fausiaina o faletusi, soso'o ai ma le fa'asologa ma le su'esu'eina o fa'amaumauga.

O le galuega atoa o auaunaga e aofia ai faʻafouga taimi moni ma faʻamaoniga o tagata o tausia e faʻamautu ai se faʻaaliga tali tali, faʻamautinoaina le sologa lelei o le faʻatinoina o galuega.

Muamua: MRNA Fa'asologa atoa-Nanopore

Sosoo ai: BMKMANU S1000 Spatial Transcriptome

E aofia ai le au'ili'iliga nei:

Puleaina o Fa'amaumauga:

o Fa'amaumauga o fa'amaumauga ma le tufatufaina o togi lelei

o Su'esu'eina o kenera i le nofoaga

o I'u lavalava

Iloiloga fa'ata'ita'i totonu:

o Gene tama'aiga

o Fa'aputuina fa'ailoga, e aofia ai fa'aitiitiga au'ili'iliga

o Su'esu'ega fa'aaliga eseese i le va o fuifui: fa'ailoaina o kenera fa'ailoga

o Fa'amatalaga aoga ma fa'atamaoaigaina o kenera fa'ailoga

Su'esu'ega va'aiga

o Toe tu'ufa'atasia o ila mai fa'ata'ita'iga uma e lua (fa'ata'ita'iga fa'ama'i ma fa'atonutonu) ma toe fa'aputu

o Fa'ailoaina o kenera fa'ailoga mo fuifui ta'itasi

o Fa'amatalaga aoga ma fa'atamaoaigaina o kenera fa'ailoga

o Fa'aaliga Eseese o le fuifui tutusa i le va o vaega

Iloiloga Fa'ata'ita'i totonu

Fa'apipi'i fa'ailoga

10x (10)

Fa'ailoga genes fa'ailoga ma fa'asoa fa'afanua

10x (12)

Su'esu'ega va'aiga

Tu'ufa'atasiga fa'amaumauga mai vaega uma e lua ma toe fa'avasega

Fa'ailoga genes o fuifui fou

Su'esu'e le alualu i luma ua fa'afaigofieina e le BMKGene's spatial transcriptomics service e le 10X Visium I nei fa'asalalauga fa'aalia:

Chen, D. et al.(2023) 'mthl1, a mafai Drosophila homologue o mammalian adhesion GPCRs, o loʻo aʻafia i le antitumor reactions i injected oncogenic sela i lago', Taualumaga a le National Academy of Sciences o le Iunaite Setete o Amerika, 120(30), i.e2303462120.doi: /10.1073/pnas.2303462120

Chen, Y. et al.(2023) 'STEEL e mafai ai ona fa'avasegaina fa'amaumauga maualuga o fa'amaumauga fa'asalalau fa'asalalau', Briefings in Bioinformatics, 24(2), itulau 1–10.doi: 10.1093/BIB/BBAD068.

Liu, C. et al.(2022) 'A spatiotemporal atlas of organogenesis in the development of orchid flowers', Nucleic Acids Research, 50(17), itulau 9724-9737.doi: 10.1093/NAR/GKAC773.

Wang, J. et al.(2023) 'Integrating Spatial Transcriptomics and Single-nucleus RNA Sequencing Fa'aalia le Ta'iala Fa'ata'ita'i Fa'apitoa mo Leiomyoma Uterine', International Journal of Biological Sciences, 19(8), itulau 2515-2530.doi: 10.7150/IJBS.83510.