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PacBio-Full-length Transcriptome (Leai Fa'asinoga)
Amplicon (16S / 18S / ITS) faʻavae ua atiina ae ma tausaga o le poto masani i suʻesuʻega faʻavae microbial diversity, lea e aofia ai le faʻavasegaina o suʻesuʻega faavae ma auʻiliʻiliga faʻapitoa: auʻiliʻiliga faavae e aofia ai le auʻiliʻiliga autu o suʻesuʻega microbial o loʻo iai nei, o le auʻiliʻiliga o mea e mauoa ma atoatoa, ma fa'ai'uga au'ili'ili o lo'o tu'uina atu ile tulaga o lipoti o poloketi;O mea o lo'o i totonu o au'ili'iliga fa'apitoa e 'ese'ese.E mafai ona filifilia faʻataʻitaʻiga ma faʻamaufaʻailoga e mafai ona faʻatulagaina fetuutuunai e tusa ai ma le lipoti o suʻesuʻega autu ma le faʻamoemoega o suʻesuʻega, e iloa ai manaʻoga faʻapitoa.Pupuni faiga fa'aoga, faigofie ma vave.
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PacBio-Full-length Transcriptome (Leai Fa'asinoga)
Avea fa'amaumauga fa'asologa o le Pacific Biosciences (PacBio) Isoform e fai ma fa'aoga, e mafai ai e lenei App ona fa'ailoa fa'asologa o tusitusiga atoa (e aunoa ma se fa'apotopotoga).E ala i le fa'avasegaina o fa'asologa atoa e fa'asaga i le genome fa'asinomaga, e mafai ona fa'amaninoina fa'amaumauga e genes ua iloa, transcripts, coding regions, ma isi. I lenei tulaga, e sili atu le fa'amaoniaina o mRNA fausaga, e pei o isi splicing, ma isi, e mafai ona ausia.Su'esu'ega fa'atasi ma fa'amaumauga fa'asologa fa'asologa o le NGS e mafai ai ona fa'amalamalama atili fa'amatalaga ma fa'atatauina sa'o i fa'amatalaga i le fa'asologa o tusitusiga, lea e tele fa'amanuiaga i fa'amatalaga fa'a-va'aiga i lalo ma su'esu'ega fa'atino.
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Meafaigaluega
BMKCloud o se taʻutaʻua bioinformatic faʻavae e tuʻuina atu le tasi-stop solution mo polokalame genomic, lea e faʻatuatuaina lautele e tagata suʻesuʻe i malae eseese e aofia ai fomaʻi, faʻatoʻaga, siosiomaga, ma isi. , punaoa faakomepiuta, faʻamaumauga a le lautele, bioinformtic online courses, etc. BMKCloud o loʻo i ai le tele o mea faʻaoga masani o le bioinformatic e aofia ai faʻamatalaga gene, meafaigaluega faʻasolosolo faʻasolosolo, ncRNA, faʻatonuga lelei o faʻamaumauga, faʻapotopotoga, alignment, faʻapipiʻiina o faʻamatalaga, suiga, fuainumera, faʻasologa o ata, faʻasologa auiliiliga, ma isi.
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RNA laiti
O tama'i RNA o se ituaiga o RNA e le fa'ailogaina ma le umi ole 18-30 nt, e aofia ai le miRNA, siRNA ma le piRNA.O nei tamaʻi RNA ua faʻasalalau tele e aʻafia i faiga faʻaola eseese e pei o le faʻaleagaina o le mRNA, faʻafefe faʻaliliuga, faʻavae heterochromatin, ma isi. O suʻesuʻega faʻasologa o SmallRNA ua faʻaaogaina lautele i suʻesuʻega i le atinaʻeina o manu / laau, faʻamaʻi, siama, ma isi. fa'asologa o su'esu'ega fa'asologa o lo'o i ai fa'amaumauga masani ma fa'asili fa'amaumauga.I luga o faʻavae o faʻamaumauga RNA-seq, suʻesuʻega masani e mafai ona ausia le faʻamaoniga ma le vavalo o le miRNA, faʻasologa o faʻasologa o le miRNA, faʻamatalaga ma faʻamatalaga faʻamatalaga.O su'esu'ega fa'apitoa e mafai ai ona su'esu'e ma su'esu'eina le miRNA, fa'atupuina o ata o Venn, miRNA ma le fausiaina o feso'ota'iga gafa.
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NGS-WGS (Illumina/BGI)
NGS-WGS ose genome re-sequencing analysis platform, lea e atiaʻe i luga o le faavae o le poto masani i Biomarker Technologies.O lenei fa'aoga faigofie e mafai ai ona vave tu'uina atu se su'esu'ega tu'ufa'atasiga e ala i le na'o le fa'atulagaina o nai fa'amaufa'ailoga autu, lea e fetaui mo fa'amaumauga fa'asologa o DNA e maua mai i le Illumina platform ma le BGI sequencing platform.O lenei fa'avae o lo'o fa'apipi'iina i luga o le 'au'aunaga fa'akomepiuta maualuga, lea e fa'amalosia ai su'esu'ega fa'amaumauga i se taimi fa'atapula'a.O lo'o maua le la'uina o fa'amatalaga fa'apitoa i luga ole fa'avae o su'esu'ega masani, e aofia ai le su'esu'eina o gene su'esu'e, mamanu muamua o le PCR, ma isi.
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mRNA(Fa'asinoga)
Transcriptome o le feso'ota'iga i le va o fa'amatalaga fa'a-genomic ma le puipuiga o galuega fa'aola.O tulafono faatonutonu tulaga fa'aliliu e sili ona taua ma sili ona su'esu'eina fa'atonuga o meaola.Transcriptome sequencing e mafai ona faʻasolosolo le transcriptome i soʻo se taimi i le taimi poʻo lalo o soʻo se tulaga, faʻatasi ai ma se iugafono saʻo i se nucleotide se tasi. E mafai ona faʻaalia malosi le maualuga o le faʻasologa o gene, i le taimi lava e tasi e iloa ma faʻatusatusa tusitusiga e seasea ma masani, ma tuʻuina atu faʻamatalaga faʻavae o fa'ata'ita'i tusitusiga fa'apitoa.
I le taimi nei, transcriptome sequencing tekinolosi ua faʻaaogaina lautele i agronomy, vailaʻau ma isi suʻesuʻega matāʻupu, e aofia ai manu ma laau faʻatonutonu tulafono faatonutonu, fetuunaiga siosiomaga, fegalegaleaiga puipuia, gene localization, ituaiga genetic evolution ma le tumo ma faʻamaʻi faʻamaʻi.
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Metagenomics (NGS)
O lenei fa'avae au'ili'ili ua mamanuina mo fana fana metagenomic su'esu'ega fa'amaumauga i luga o le fa'avae o tausaga o le poto masani.O lo'o i ai fa'atasi galuega fa'aogaina o lo'o iai su'esu'ega metagenomics e masani ona mana'omia e aofia ai fa'amaumauga fa'amaumauga, su'esu'ega ituaiga-tulaga, su'esu'ega tulaga-tulaga o le gene, metagenome binning, ma isi. , fa'atulagaina fa'amaufa'ailoga, fa'atupuina fa'atusa fa'apitoa, ma isi.
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LncRNA
O RNA e le fa'ailoga umi (lncRNA) o se ituaiga o tusitusiga e umi atu nai lo le 200 nt, e le mafai ona fa'ailoga polotini.O fa'amaumauga fa'aputuga e fa'ailoa mai ai o le tele o lncRNA e foliga mai e fa'atino.Fa'atekonolosi fa'asologa maualuga ma mea faigaluega su'esu'e bioinformatic e fa'amalosia ai i tatou e fa'aalia fa'asologa o le lncRNA ma fa'atulagaina fa'amatalaga sili atu ona lelei ma ta'ita'ia ai i tatou e su'e lncRNA ma galuega fa'atonutonu taua.E mitamita le BMKCloud e tu'uina atu ia matou tagata fa'atau lncRNA fa'asologa o su'esu'ega fa'asologa e ausia ai le vave, fa'atuatuaina ma fetu'una'i su'esu'ega lncRNA.
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GWAS
Genome-wide association study (GWAS) e fa'atatau i le fa'ailoaina ole kenera (genotype) e feso'ota'i ma uiga fa'apitoa (phenotype).O su'esu'ega a le GWA e su'esu'e ai fa'ailoga fa'a kenera e fa'asaga i le genome atoa o le aofa'i tele o tagata ta'ito'atasi ma va'ai fa'atasiga fa'a-genotype-phenotype e ala i su'esu'ega fa'afuainumera i le aofa'i o tagata.Ole su'esu'ega atoa-genome e mafai ona maua uma fa'asologa o kenera.Fa'atasi ma fa'amaumauga fa'apitoa, e mafai ona fa'agasolo le GWAS e fa'ailoa ai SNP e feso'ota'i phenotype, QTLs ma genes sui, lea e fa'aola malosi ai le fa'atupuina o manu/laau fa'aonaponei.O le SLAF ose fa'ata'ita'iga fa'asolosolo fa'asolosolo fa'afaigofie na fa'atupuina e le tagata lava ia, lea e maua ai fa'ailoga fa'asalalauina lautele, SNP.O nei SNP, e pei o faʻailoga mole mole, e mafai ona faʻatautaia mo suʻesuʻega faʻatasi ma uiga faʻatatau.Ose ta'iala taugofie i le fa'ailoaina o uiga lavelave e feso'ota'i ma fesuiaiga o kenera.
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Nanopore Fa'amaumauga atoa
Lavelave ma fesuia'i isi isoforms i meaola o ni faiga taua tele mo le fa'atonutonuina o le fa'aaliga o kenera ma le eseesega o polotini.O le faʻamaonia saʻo o faʻasologa o tusitusiga o le faʻavae lea mo suʻesuʻega loloto o faʻataʻitaʻiga o tulafono faatonutonu o faʻamatalaga.Nanopore sequencing platform ua manuia le aumaia o suʻesuʻega transcriptomic i le isoform-level.O lenei fa'avae au'ili'ili ua mamanuina e fa'avasega ai fa'amaumauga a le RNA-Seq na fa'atupuina ile Nanopore platform ile fa'avae ole genome fa'asino, lea e ausia ai au'ili'iliga fa'atatau ma fa'atusatusaga ile tulaga o le gene ma le fa'asologa o tusitusiga.
