BMKMANU S1000 Spatial Transcriptome
BMKMANU S1000 Spatial Transcriptome Technical Scheme
Tulaga lelei o le BMKMANU S1000
1) Fuafuaga i lalo ole telefoni: O vaega taʻitasi e puʻeina e iai> 2 miliona avanoa Faʻasalalau Faʻasalalau ma le lautele ole 2.5 μm ma le va o le 5 μm i le va o nofoaga tutotonu, faʻataga ai suʻesuʻega transcriptome faʻafanua faʻatasi ma faʻaiʻuga laiti (5 μm).
2) Su'esu'ega fa'ai'uga fa'atele: Su'esu'ega fa'atele-va'aiga fetu'una'i e amata mai i le 100 μm i le 5 μm e fo'ia ai vaega 'ese'ese o le tino ile fa'ai'uga lelei.
3) Fa'asalalauga fa'asalalau fa'apitoa: O fa'amaumauga na pu'eina mai le fa'asolo atoa o le tino e mafai ona su'esu'eina, e aunoa ma le fa'atapula'aina o numera o kenera fa'atatau ma le vaega fa'atatau.
Fa'amatalaga Auaunaga
| Faletusi | Fuafuaga fa'asologa | Fa'amatalaga Fa'amatalaga fautuaina |
| S1000 cDNA faletusi | BMKMANU S1000-Illumina PE150 | 60Gb / faʻataʻitaʻiga |
Fa'ata'ita'iga Manaoga
| Fa'ata'ita'iga | Numera | Tele | RNA Tulaga lelei |
| OCT fa'apipi'i poloka aano | 2-3 poloka / faʻataʻitaʻiga | Pe tusa.6.8x6.8x6.8 mm3 | RIN≥7 |
Mo nisi fa'amatalaga i fa'ata'ita'iga tapenaga ta'iala ma au'aunaga, fa'amolemole lagona le saoloto e talanoa i aBMKGENE tagata poto
Auala o Galuega Au'aunaga
Fa'ata'ita'iga mamanu
Fa'ata'ita'iga tu'uina atu
Fa'ata'ita'iga muamua
Fa'ailoga fa'avanoa
Fauga faletusi
Fa'asologa
Iloiloga fa'amaumauga
Au'aunaga pe a uma ona fa'atau
O faʻamaumauga na gaosia e le BMKMANU S1000 e suʻesuʻeina e faʻaaoga ai le polokalama "BSTMatrix", lea e tutoʻatasi na fuafuaina e BMKGENE, e aofia ai:
1) Fa'atupuina fa'atupu fa'amatalaga o kenera
2) HE faiga ata
3) Faʻatasi ma le pito i lalo o le vaega lona tolu polokalama mo le auiliiliga
4) E fesoasoani le "BSTViewer" i luga ole laiga e maua ai fa'ai'uga fa'aaliga i fa'ai'uga eseese.
1.Spot fa'aputuina
2. Fa'asoa fanua
Note: I'ugafonotulaga=13 (100 µm, taumatau); 7 (50 µm, tauagavale)
3. Fa'ailoga fa'ailoga fa'aputuga fa'afu'efu fa'afanua vevela
4.Inter-faʻataʻitaʻiga suʻesuʻega faʻamatalaga
