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条形 fu'a-03

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Chromatin Immunoprecipitation Sequencing (ChIP-seq)

O le ChIP-Seq e tu'uina atu ai le fa'avasegaina o le genome-lautele o taula'iga DNA mo suiga fa'asolopito, fa'asologa o tusitusiga, ma isi polotini e feso'ota'i DNA.E tu'ufa'atasia le filifilia o le chromatin immuno-precipitation (ChIP) e toe fa'aleleia ai fa'alavelave fa'apolotini-DNA fa'apitoa, fa'atasi ai ma le mana o le fa'asologa o le isi augatupulaga (NGS) mo le fa'asologa maualuga o le DNA toe maua.E le gata i lea, talu ai o le protein-DNA lavelave e toe maua mai sela ola, e mafai ona faʻatusatusa nofoaga o loʻo fusifusia i ituaiga sela eseese ma aano, pe i lalo o tulaga eseese.O talosaga e amata mai i tulafono faatonutonu tusitusia i auala tau atina'e i faiga fa'ama'i ma tua atu.

Tulaga: Illumina NovaSeq Platform


Au'aunaga Fa'amatalaga

Bioinformatics

I'uga Fa'aaliga

Tulaga Auaunaga

● Platform: Illumina NovaSeq Platform, PE150

● Ituaiga faletusi: 100-500bp fa'aofi le faletusi DNA (fa'alagolago i le fa'asoa maualuga)

● Fuafuaga fa'asologa: Fa'atasi-Fa'ai'uga 150 bp

● Fautuaina faʻamatalaga faʻamatalaga: 6 Gb raw data / faʻataʻitaʻiga.

Fa'ata'ita'iga Manaoga

Aofa'i DNA: ≥ 70 ng, pito maualuga o le 100 i le 500 bp

Fa'ata'ita'iga voluma: ≥ 10 μl

OD260/280 = 1.8 i le 2.0 e aunoa ma le faʻaleagaina poʻo le RNA faʻaleagaina

Fa'ata'ita'iga galuega fa'atino

图片1

Auala o Galuega Au'aunaga

fa'ata'ita'iga tu'uina atu

Fa'ata'ita'iga tu'uina atu

Sauniuniga Faletusi

Fausia faletusi

Fa'asologa

Fa'asologa

Iloiloga fa'amaumauga

Iloiloga fa'amaumauga

A uma le fa'atauga Auaunaga

Au'aunaga pe a uma ona fa'atau


  • Muamua:
  • Sosoo ai:

  • 表观-01

    1. Fa'atamaoaigaina pito i luga

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    2. MA fasipepa ole tumutumu eseese

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    3.KEGG fa'amatalaga

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    4. GO fa'avasegaga

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