CircRNA Sequencing-Illumina
Vaega
● fa'aitiitiga o le rRNA soso'o ai ma tapenaga o faletusi fa'atonu, fa'aagaaga fa'amaumauga fa'asologa fa'apitoa.
● Bioinformatic workflow e mafai ai le circRNA vavalo ma faʻamatalaga faʻatusatusaga
Tulaga Auaunaga
●Sili A'oa'oga RNA Faletusi:Matou te faʻaogaina le faʻaitiitia o le rRNA nai lo le faʻaitiitia o le RNA laina i la matou sauniuniga aʻo leʻi faia le faletusi, faʻamautinoa o faʻamaumauga faʻasologa e aofia ai e le gata o le circRNA ae faʻapea foʻi ma le mRNA ma le lncRNA, e mafai ai ona suʻesuʻeina faʻatasi o nei faʻamaumauga.
●Filifiliga Su'esu'ega o Fesootaiga RNA Endogenous Competitive (ceRNA).: Tu'uina atu o fa'amatalaga loloto i faiga fa'atonutonu telefoni feavea'i
●Tomai Tele: Fa'atasi ai ma se fa'amaumauga o fa'agaioiga i luga ole 20,000 fa'ata'ita'iga ile BMKGENE, fa'ata'amilo i ituaiga fa'ata'ita'iga 'ese'ese ma poloketi lncRNA, e aumaia e la tatou 'au le tele o le poto masani i galuega uma.
●Pulea lelei: Matou te fa'atinoina le fa'atonuga autu i la'asaga uma, mai le fa'ata'ita'iga ma tapenaga faletusi i le fa'asologa ma bioinformatics. O lenei mata'ituina lelei e fa'amautinoa ai le tu'uina atu o fa'ai'uga maualuluga.
● Post-Fa'atau Lagolago: O la matou tautinoga e faʻalautele atu i tua atu o le faʻamaeʻaina o le poloketi ma le 3-masina pe a maeʻa le faʻatau atu taimi o auaunaga. I le taimi lea, matou te ofoina atu le tulitatao o poloketi, fesoasoani faʻafitauli, ma Q&A sauniga e faʻafeiloaʻi ai soʻo se fesili e fesoʻotaʻi ma iʻuga.
Fa'ata'ita'iga Mana'oga ma le Tu'uina atu
| Faletusi | Fa'avae | Fa'amatalaga fautuaina | Fa'amatalaga QC |
| rRNA ua uma le faletusi faasinoala | Illumina PE150 | 16-20 Gb | Q30≥85% |
Fa'ata'ita'iga Mana'oga:
Nucleotides:
| Conc.(ng/μl) | Aofa'i (μg) | Mama | Amiotonu |
| ≥ 80 | ≥ 0.8 | OD260/280=1.7-2.5 OD260/230=0.5-2.5 Fa'atapula'a pe leai se polotini po'o le fa'aleagaina o DNA o lo'o fa'aalia ile gel. | RIN≥6.0; 5.0≥28S/18S≥1.0; fa'atapula'a pe leai se maualuga maualuga |
● La'au:
A'a, Po'o Po'o Petal: 450 mg
Laulaau po'o Fatu: 300 mg
Fualaau: 1.2 g
● Manu:
Loto po'o le Intestinal: 450 mg
Viscera po'o Fai'ai: 240 mg
Muso: 600 mg
Ivi, Laulu po'o Pa'u: 1.5g
● Arthropods:
Iniseti: 9g
Crustacea: 450 mg
● toto atoa:2 paipa
● Selula: 106 sela
● Serum ma Plasma: 6 mL
Fautuaina Fa'ata'ita'iga Tu'uina atu
Containe: 2 ml centrifuge tube (E le fautuaina le pepa apa)
Fa'ailoga fa'ata'ita'iga: Vaega+fa'atusa eg A1, A2, A3; B1, B2, B3.
utaina:
1. Fa'amago-aisa: Fa'ata'ita'iga e mana'omia ona fa'aputu i taga ma tanu i le aisa-mago.
2. RNAstable paipa: RNA faʻataʻitaʻiga e mafai ona faʻamago i le RNA stabilization tube (faʻataʻitaʻiga, RNAstable®) ma lafo i le vevela potu.
Auala o Galuega Au'aunaga
Fa'ata'ita'iga mamanu
Fa'ata'ita'iga tu'uina atu
RNA su'ega
Fauga faletusi
Fa'asologa
Iloiloga fa'amaumauga
Au'aunaga pe a uma ona fa'atau
Bioinformatics
va'aiga circRNA: tufatufa chromosomal
circRNAs Fa'aaliga Eseese – Fuafuaga o mauga mu
circRNAs Fa'aalia Eseese - fa'aputuga fa'afa'atonu
Fa'atamaoaigaina fa'atino o genes talimalo a le circRNA
Su'esu'e le alualu i luma o su'esu'ega na fa'afaigofieina e le BMKGene' circRNA sequencing services e ala i le aoina o fa'asalalauga.
Wang, X. et al. (2021) 'CPSF4 fa'atonutonu le fa'atupuina o le circRNA ma le microRNA mediated gene silencing in hepatocellular carcinoma', Oncogene 2021 40:25, 40(25), itulau 4338-4351. doi: 10.1038/s41388-021-01867-6.
Xia, K. et al. (2023) 'X oo-responsive transcriptome o loʻo faʻaalia ai le matafaioi a le RNA133 lapotopoto i faʻamaʻi faʻamaʻi e ala i le faʻatonutonuina o faʻamatalaga o le OsARAB i le araisa', Phytopathology Research, 5 (1), itulau 1-14. doi: 10.1186/S42483-023-00188-8/ATA/6.
Y, H. et al. (2023) 'O le CSSF3 e faʻatulagaina le paleni o faʻasalalauga faʻasalalau ma laina laina i le hepatocellular carcinoma'. doi: 10.21203/RS.3.RS-2418311/V1.
Zhang, Y. et al. (2023) 'Suʻesuʻega maeʻaeʻa o circRNAs i le cardiomyopathy cirrhotic aʻo leʻi faʻapipiʻiina le ate', International Immunopharmacology, 114, itulau. 109495. doi: 10.1016/J.INTIMP.2022.109495.
