Illumina faletusi na faia muamua
Vaega
●Fa'avae:Illumina NovaSeq 6000 ma NovaSeq X Plus
●Fa'asologa o faiga:PE150 ma le PE250
●Pulea lelei o faletusi a'o le'i fa'asologa
●Fa'asologa o fa'amaumauga QC ma le tu'uina atu:tu'uina atu o le QC lipoti ma fa'amatalaga mata'utia i le fa'asologa vaveq pe a mae'a le fa'avasegaina ma le fa'amamaina o le Q30 faitau
Tulaga Auaunaga
●Fetuuna'iga o auaunaga Fa'asologa:e mafai e le tagata fa'atau ona filifili e fa'asologa i le laina, tafe sela, po'o le aofa'i o fa'amaumauga e mana'omia (fa'asologa o le laina).
●Le poto masani i luga ole tulaga faʻasologa o le Illumina:fa'atasi ai ma le faitau afe o galuega tapuni ma ituaiga eseese.
●Tuuina atu o le fa'asologa o lipoti QC:fa'atasi ai ma fua fa'atatau, sa'o fa'amaumauga ma le fa'atinoga atoa o le fa'asologa o galuega.
●Fa'asologa fa'asologa o tagata matutua:fa'atasi ai ma sina taimi puupuu.
●Pulea lelei: matou te faʻatinoina manaʻoga QC e faʻamautinoa ai le tuʻuina atu o taunuʻuga maualuga maualuga.
Fa'ata'ita'iga Fa'avae
| Fa'avae | Foma'i Cell | Faiga fa'asologa | Vaega | Fuafuaga fa'atatau |
| NovaSeq X | 10B (8 laina) | PE150 | Lane Nofofua Auala Vaega | 375Gb / Lane |
| 25B (8 laina) | PE150 | Lane Nofofua Auala Vaega | 1000 Gb/Lane | |
| NovaSeq 6000 | SP Flow cell (2 laina) | PE250 | Foma'i Cell Lane Nofofua Auala Vaega | 325-400 M faitau / Lane |
| S4 Flow cell (4 laina) | PE150 | Foma'i Cell Lane Nofofua Auala Vaega | ~ 800 Gb / Lane |
Fa'ata'ita'iga Manaoga
| Aofa'i Fa'amatalaga (X) | Fa'atotonuga (qPCR/nM) | Volume | |
| Fa'asologa o Auala Fa'apitoa
| X ≤ 10 Gb | ≥ 1 nM | ≥ 25 μl |
| 10 Gb < X ≤ 50 Gb | ≥ 2 nM | ≥ 25 μl | |
| 50 Gb < X ≤ 100 Gb | ≥ 3 nM | ≥ 25 μl | |
| X> 100 Gb | ≥ 4 nM | ≥ 25 μl | |
| Fa'asologa o Lane | Per Lane | ≥ 1.5 nM / Faletusi vaitaele | ≥ 25 μl / Faletusi vaitaele |
I le faaopoopo atu i le faʻatonuga ma le aofaʻi atoa, e manaʻomia foʻi se faʻataʻitaʻiga maualuga talafeagai.
Fa'aaliga: O le fa'asologa o laina o faletusi laiti eseese e mana'omia ai le PhiX spike-in ina ia mautinoa le malosi o le vala'au.
Matou te fautuaina le tu'uina atu o faletusi ua tu'ufa'atasia e fai ma fa'ata'ita'iga. Afai e te mana'omia le BMKGENE e fai le fa'aputuga o faletusi, fa'amolemole va'ai
o mea e mana'omia mo le fa'avasegaina o auala fa'afa'aga.
Tele o le Faletusi (Peak fa'afanua)
Ole pito maualuga e tatau ona i totonu ole 300-450 bp.
O faletusi e tatau ona tasi le tumutumu autu, leai se mea fa'apipi'i fa'aleagaina ma leai ni dimer muamua.
Fa'amolemole fa'afeso'ota'i mai pe a le fetaui au fa'ata'ita'iga i mea e mana'omia amata.
Au'aunaga Galuega
Pulea lelei o faletusi
Fa'asologa
Puleaina lelei o faamatalaga
Tuuina atu o poloketi
Lipoti QC Faletusi
E tu'uina atu se lipoti i le tulaga lelei o le faletusi a'o le'i fa'asologa, iloilo le aofa'i o faletusi, ma vaevaega.
Fa'asologa o lipoti QC
Laulau 1. Fa'amaumauga ile fa'asologa o fa'amaumauga.
| Fa'ata'ita'iga ID | BMKID | Faitauga mata'utia | Fa'amatalaga Mata'utia (bp) | Faitau mama (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Ata 1. Tu'ufa'atasiga lelei ma faitau i fa'ata'ita'iga ta'itasi
Ata 2. Tufatufaina o mataupu faavae
Ata 3. Tufatufaina o mataupu faitau ile fa'asologa o fa'amaumauga
