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Illumina ma le BGI

Illumina sequencing technology, fa'avae i le Sequencing by Synthesis (SBS), o se fa'afouga a le NGS i le lalolagi atoa, e nafa ma le fa'atupuina o le 90% o fa'amaumauga fa'asologa o le lalolagi.O le mataupu faavae o le SBS e aofia ai ata fa'ailoga fa'aigoa fa'alelei toe fa'afo'i a'o fa'aopoopo i ai le dNTP ta'itasi, ma soso'o ai loa ona fa'ataga e fa'ataga ai le tu'ufa'atasia o le isi fa'avae.Faatasi ai ma dNTPs uma e mafai ona toe fa'afo'i fa'amuta ua iai i ta'amilosaga fa'asologa ta'itasi, o tauvaga fa'anatura e fa'aitiitia ai le fa'aituau fa'atasi.O lenei tekinolosi fa'akomepiuta e lagolagoina uma faletusi e tasi-faitau ma faletusi fa'alua-i'uga, fa'amalieina le tele o fa'aoga genomic.Ole fa'asologa o le Illumina e mafai ona fa'aogaina ma le sa'o o lo'o fa'atulagaina e fai ma maatulimanu ile su'esu'ega genomics, fa'amalosia le au saienitisi e fa'amatalaina le lavelave o genomes ma fa'amatalaga e le mafaatusalia ma le lelei.

DNBSEQ, atiaʻe e le BGI, o se isi faʻatekonolosi NGS fou ua mafai ona faʻaititia atili i lalo tau faʻasologa ma faʻateleina le gaosiga.O le tapenaga o faletusi DNBSEQ e aofia ai le vaevaega o DNA, le saunia o le ssDNA ma le fa'alauteleina o le li'o ta'avale e maua ai le DNA nanoballs (DNB).Ona utaina lea o nei mea i luga o se mea malō ma sosoo ai ma le fa'asologa e ala i le tu'ufa'atasiga o le Su'esu'ega-Tala'au (cPAS).

O la matou 'au'aunaga fa'asologa o faletusi e fa'afaigofie ai tagata fa'atau i le sauniaina o a latou faletusi fa'asologa mai fa'apogai eseese (mRNA, genome atoa, amplicon, ma isi).Mulimuli ane, o nei faletusi e mafai ona lafo i o tatou nofoaga faʻasologa mo le faʻatonutonuina lelei ma le faʻasologa i Illumina poʻo BGI platforms.


Au'aunaga Fa'amatalaga

Fa'aaliga Fa'aaliga

Vaega

Fa'avae:Illumina NovaSeq 6000, NovaSeq, HiSeq X Ten ma le BGI-DNB-T7

Fa'asologa o faiga:PE50, PE100, PE150, PE250

Pulea lelei o faletusi a'o le'i fa'asologa

Fa'asologa o fa'amatalaga tu'uina atu ma QC:tu'uina atu o le QC lipoti ma fa'amatalaga mata'utia i le fa'asologa vaveq pe a mae'a le fa'avasegaina ma le fa'amamaina o le Q30 faitau.

 

Tulaga Auaunaga

Fetuuna'iga o auaunaga Fa'asologa:e mafai e le tagata fa'atau ona filifili e fa'asologa i le ala, tafe sela po'o le aofa'i o fa'amaumauga.

Fetuuna'iga o tulaga:O faletusi DNB e mafai ona faʻafeiloaʻi i luga ole Illumina platforms

Le poto masani i luga ole tulaga faʻasologa o le Illumina:fa'atasi ai ma le faitau afe o galuega tapuni ma ituaiga eseese. 

Tuuina atu o le fa'asologa o lipoti QC:fa'atasi ai ma fua fa'atatau, sa'o fa'amaumauga ma le fa'atinoga atoa o le fa'asologa o galuega.

Fa'asologa fa'asologa o tagata matutua:fa'atasi ai ma sina taimi puupuu.

Pulea lelei: matou te faʻatinoina manaʻoga QC e faʻamautinoa ai le tuʻuina atu o taunuʻuga maualuga maualuga.

Fa'ata'ita'iga Mana'oga*

Fa'asologa o Auala Fa'apitoa

Aofa'i Fa'amatalaga (X)

Fa'atotonuga (qPCR/nM)

Volume

X ≤ 50 Gb

≥ 2 nM

≥ 20 μl

50 Gb ≤ X <100 Gb

≥ 3 nM

≥ 20 μl

X ≥ 100 Gb

≥ 4 nM

≥ 20 μl

Lane Tasi (Illumina)

Fa'avae

Fa'atotonuga (qPCR/nM)

Volume

HiSeq X Sefulu

≥ 2 nM

≥ 20 μl

NovaSeq 6000 SP

≥ 1 nM

≥ 25 μl

NovaSeq 6000 S4

≥ 1.5 nM

≥ 25 μl

NovaSeq X

≥ 1.5 nM

≥ 25 μl

BGI-DNBSEQ-T7

≥ 1.5 nM

≥ 25 μl

 I le faaopoopo atu i le faʻatonuga ma le aofaʻi atoa, e manaʻomia foʻi se faʻataʻitaʻiga maualuga talafeagai.

 

Fa'amolemole fa'afeso'ota'i mai pe a le fetaui au fa'ata'ita'iga i mea mana'omia amata.

Au'aunaga Galuega

sauniuniga faataitai

Pulea lelei o faletusi

Fa'asologa

Fa'asologa

Iloiloga fa'amaumauga

Puleaina lelei o faamatalaga

Fa'ata'ita'iga QC

Tuuina atu o poloketi


  • Muamua:
  • Sosoo ai:

  • Lipoti QC Faletusi

    E tu'uina atu se lipoti i le tulaga lelei o le faletusi a'o le'i fa'asologa, iloilo le aofa'i o faletusi, ma vaevaega.

     

    Fa'asologa o lipoti QC

     

    Laulau 1. Fa'amaumauga ile fa'asologa o fa'amaumauga.

    Fa'ata'ita'iga ID

    BMKID

    Faitauga mata'utia

    Fa'amatalaga Mata'utia (bp)

    Faitau mama (%)

    Q20(%)

    Q30(%)

    GC(%)

    C_01

    BMK_01

    22,870,120

    6,861,036,000

    96.48

    99.14

    94.85

    36.67

    C_02

    BMK_02

    14,717,867

    4,415,360,100

    96.00

    98.95

    93.89

    37.08

    Ata 1. Tu'ufa'atasiga lelei ma faitau i fa'ata'ita'iga ta'itasi

    A9

    Ata 2. Tufatufaina o mataupu faavae

    A10

    Ata 3. Tufatufaina o mataupu faitau ile fa'asologa o fa'amaumauga

    A11

     

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