Tulaga | Lautele Tele | Faʻaaogaina Faʻamatalaga Faʻamaumauga (i le sela) | Tasi-faavae sao | Faatatauga |
Nanopore | 8kbb, 10kb, 20kb, Uldalong, CDNA-PCR | 70-90GB / sela | 85-92% | SV Valaʻau, Devo, atoa-umi sequeding, iso-seq, Gene mai le faʻailoga o le DNA MUA |
● O le silia ma le 5 tausaga le poto masani i luga o le Pacbio linquering o le faitau afe o tapuni poloketi ma ituaiga eseese.
● Bmkgene o se paaga aloaia o Oxford Nanopore, ma le lua sipeneta RNA / DNA tusi Faamaonia.
● O loʻo iai ni ata autu o se vaega o ni vaega ma le atoatoa masini ma lava senie o loʻo fesoasoani.
● E faʻavae i luga o le isumu Nanopore, sili atu nai lo le 10 manu ma laʻau Denovo suʻesuʻe ua lomia i loʻo lolomiina i mea faʻavaomalo.
Faʻataʻitaʻiga ituaiga | Aofaiga | Cynderingrion (qubit ®) | Fua | Amio mama | Isi |
Genomic dna | Faʻalagolago i faʻamatalaga manaʻoga | ≥20ng / μl | ≥15μl | Od260 / 280 = 1.7-2.2; Od260 / 2300≥1.5; Manino pea i le 260 nm, leai ni mea leaga | Manaʻomia e manaʻomia le fuaina e le qubit ma qubit / nanopore ≤ 2 |
Aofai RNA | ≥1.2μg | ≥100μg / μl | ≥15μl | Od260 / 280 = 1.7-2.5; Od260 / 230 = 0.5-2.5; leai ni vaiinoa | Riin tau ≥7.5 |
Faʻamatalaga o faʻamatalaga o le DNA faʻataʻitaʻiga
Lisi 1. Fuafanua i luga o faʻamatalaga mama.
Bmkid | rawseqnum | rassumond | mama | Faamamaina | faamamaina 50n | mama90len | mama | faamama | Faamamaina |
DNA_BMK01 | 1,218,239 | 26.37 | 1,121,736 | 25.90 | 28,014 | 15,764 | 23,090 | 143,181 | 9 |
Faʻamatalaga o faʻamatalaga o le RNA faʻataʻitaʻiga
Lisi 1. Fuafanua i luga o faʻamatalaga mama.
Igoa ole igoa | Tagata kalani | Faitau tusi | Basenum | N50 | Uiga o le | Maxlength | Uiga o le |
Rna_bmk001 | C2 | 8,947,708 | 4,047,230,083 | 398 | 452 | 129,227 | Q12 |
Ata 1. Faitau le umi tufatufaina
Ata 2. Mafuaaga sikoa tufatufaina o le mama faamatalaga
Ata 3. Umi ma le lelei sikoa tufatufaina o le mama faamatalaga