TGuide Smart Magnetic Plant RNA Kit
Numera fa'amaumauga / afifiina
| Pusi. leai | ID | Nu. o sauniuniga |
| 4993552(kesi) | DP662-DE | 48 |
Ta'iala filifilia mo le TGuide S16
| Nuclei Acid | Ituaiga Fa'ata'ita'i | Igoa Oloa | Tele o le afifiina (sauniuniga) kesi/ ipu | Pusi nu. Kesi/papa |
| DNA | Tisu Manu | TGuide Smart Magnetic Tissue DNA Kit | 48/96 | 4993547/4995038 |
| DNA | La'au ma Fatu | TGuide Smart Magnetic Plant DNA Kit | 48 | 4993548 |
| DNA | Eleele/Stool | TGuide Smart Soil/Stool DNA Kit | 48 | 4993549 |
| DNA | Gel & PCR oloa | TGuide Smart DNA Purification Kit | 48 | 4993550 |
| DNA | Toto Atoa | TGuide Smart Blood Genomic DNA Kit | 48/96 | 4993703/4995206 |
| DNA | Cell/Swab/Mago, ma isi | TGuide Smart Universal DNA Kit | 48/96 | 4993704/4995040 |
| RNA | Toto/Sela/Tisu | TGuide Smart Blood/Cell/Tissue RNA Kit | 48/96 | 4993551/4995043 |
| RNA | La'au ma Fatu | TGuide Smart Magnetic Plant RNA Kit | 48 | 4993552 |
| DNA/RNA | Suavai e leai se sela (Serum, ma isi) | TGuide Smart Viral DNA/RNA Kit | 48/96 | 4993702/4995207 |
Folasaga o oloa
Faʻamama le maualuga-maea, maualuga-mama, maualuga le aofaʻi o le RNA mai le toto / sela / gaʻo.
Leai ni vaila'au o'ona pei ole phenol/chloroform o lo'o iai
O le RNA na maua e maualuga le mama ma e mafai ona faʻaoga saʻo mo le suʻeina o pu, faʻasologa maualuga ma isi suʻega.
Vaega
Super faigofie e faʻaaoga
Leai se isi galuega paipa.
E mafai ona fetu'una'i le voluma elution pe a mana'omia.
O le faʻapipiʻiina ma le faʻaogaina e manaʻomia ai sina aʻoaʻoga. Faatasi ai ma polokalame na muai faʻapipiʻiina, tatala le pusa, filifili le protocol ma faʻatautaia lau suʻega. Polokalama ua saunia-e-fa'aoga, ma fa'apitoa fo'i.
Reagents mua'i utaina ma fa'afetaui mea fa'aaoga fa'aaoga.
O le tagofiaina o vailaʻau e faʻaumatia i le tulaga sili e faʻamautinoa ai le saogalemu o le gaioiga.
Fa'aaliga Fa'aaliga
A'a o le saito atoa RNA fa'amama
Fa'ata'ita'iga tele: 100 mg
Fa'ata'ita'iga fa'ata'ita'iga: oloina i le fa'asusu vai po'o le vevela o le fa'aogaina o le homogenizer
Akaraose gel fa'asalaina:2%(TAE)
Tuʻuina le voluma: 2 μl
Faailoga: Faailoga IV, TIANGEN
Fa'ata'ita'iga taunu'uga: fa'aaoga TGuide S16 ausia ma reagent 4993552 e otometi lava ona aveese mai le RNA atoa o a'a saito e maua lelei ma maualuga le mama. I lenei faʻataʻitaʻiga, e tusa ma le 20 μg o le nucleic acid na maua mai 100 mg aʻa saito, faʻatasi ai ma le OD260 / OD280 i le 2.0 ~ 2.1, ma le OD260 / OD230> 2.0.
Aofa'i RNA mai fa'ata'ita'iga la'au eseese
Fa'ata'ita'iga tele: 100 mg
Fa'ata'ita'iga fa'ata'ita'iga: homogenizer maualalo vevela
Fa'asalaina gel agarose:1% (TAE)
Tuʻuina le voluma: 1 μl
M: Maka III, TIANGEN
1-4: fatu sana 5-8: lau sana 9-12: a'a kapisi 13-16: soya.
O fa'ata'ita'iga muamua e lua na maua mai e le pusa fa'ata'ita'i fa'ailo-koluma ma fa'ata'ita'iga mulimuli e lua na maua mai e TGuide S16.
I'uga o fa'ata'ita'iga: o le fua fa'atatau o la'au RNA na saunia e TGuide S16 fa'atasi ai ma le reagent 4993552 e tutusa pe sili atu nai lo le mea e maua mai i le pusa fa'ata'ita'i laina. O le mama ma le faamaoni e matua tutusa lava. O lea la, e mafai ona fa'aogaina le TGuide S16 e su'e ai le RNA atoa o la'au e suitulaga i le vaifofo fa'avae fa'avae.
