Fungal Genome

Biomarker Technologies e tuʻuina atu suʻesuʻega o le genome, genome lelei ma faʻamaeʻaina genome o gaʻo e faʻatatau ile sini suʻesuʻe.Genome sequencing, faʻapotopotoga ma faʻamatalaga aoga e mafai ona ausia e ala i le tuʻufaʻatasia o le Generation sequencing + Faʻasologa o augatupulaga lona tolu e ausia ai le maualuga o le genome assembly.E mafai fo'i ona fa'aogaina le tekonolosi Hi-C e fa'afaigofie ai le fa'apotopotoina o le genome ile tulaga ole chromosome.

Fa'avae:PacBio Sequel II

Nanopore PromethION P48

Illumina NovaSeq Platform

Faafesootai matou

Au'aunaga Fa'amatalaga

I'uga Fa'aaliga

Tulaga Auaunaga

● Ta'iala fa'asologa e tele e avanoa mo sini su'esu'e eseese

● E sili le poto masani i le fuga genome assembly ma sili atu i le 10,000 pene-complete genome ua tuufaatasia.

● Tomai fa'apolofesa ina ua uma ona fa'atau atu 'au lagolago fa'apitoa fa'amalieina mana'oga fa'apitoa su'esu'ega.

Fa'amatalaga Auaunaga

Auaunaga	Fuafuaga Fa'asologa	Tulaga mautinoa	Fa'atatau le taimi e liliu ai
Fa'afanua lelei fungal	Illumina 50X+Nanopore 100X	Contig N50≥2 Mb	35 aso faigaluega
Fa'afanua lelei fungal	PacBio HiFi 30X	Contig N50≥2 Mb	35 aso faigaluega
Fungal pene-uma faafanua	Illumina 50X+Nanopore 100X(Pacbio HiFi 30X)+Hi-C 100X	fua faatatau o le taula o Chromosome >90%	45 aso faigaluega

Bioinformatics su'esu'ega

● Pulea lelei o fa'amaumauga mata'utia

● Genome assembly

● Su'esu'ega vaega o le Genome

● Fa'amatalaga galuega o kene

● Su'esu'ega genomic fa'atusatusa

Fa'ata'ita'iga Mana'oga ma le Tu'uina atu

Fa'ata'ita'iga Mana'oga:

MoDNA extracts:

Ituaiga Fa'ata'ita'i	Aofaiga	Fa'atotonuga	Mama
DNA extracts	+ 1.2 μg	+ 20 ng/μl	OD260/280= 1.6-2.5

Mo faʻataʻitaʻiga o mea'ai:

Fa'ata'ita'iga ituaiga

Fautuaina fa'ata'ita'iga togafitiga

Fa'ata'ita'iga teuina ma uta

Unicellular Fungus

Mata'ituina le fefete i lalo o le microscope ma aoina i latou i la latou vaega fa'atele

Faʻafeiloaʻi le aganuʻu (o loʻo i ai le 3-4.5e9 sela) i totonu o le 1.5 poʻo le 2 ml eppendorf.(Tuu i luga ole aisa)

Centrifuge le paipa mo le 1 min i le 14000 g e aoina ai siama ma aveese ma le faaeteete le supernatant

Fa'amaufa'ailoga le paipa ma fa'a'aisa le siama ile fa'asusu vai mo le itiiti ifo ile 1-3 itula.Teu le paipa ile -80 ℃ pusaaisa.

Fa'a'aisa fa'ata'ita'iga ile sua vai mo le 3-4 itula ma teu ile fa'asusu vai po'o le -80 tikeri ile fa'aagaga umi.E manaʻomia le faʻataʻitaʻiga o felauaiga ma le aisa mago.

Macro Fungus

E fautuaina le fa'apipi'i i le vaega o lo'o ola malosi.

Fufulu le aano i le vai e leai se endotoxin, ona 70% lea o le ethonal.

Fa'asaoina le fa'ata'ita'iga ile cryo-tubes.