Genome-wide Association Analysis
Fa'asologa o galuega
Tulaga Auaunaga
●Tomai Tele ma fa'amaumauga fa'asalalau: fa'atasi ai ma le fa'aputuina o le poto masani i le GWAS, BMKGene ua mae'a le fiaselau o ituaiga galuega fa'atino i le faitau aofa'i o su'esu'ega a le GWAS, fesoasoani i tagata su'esu'e e fa'asalalau tala e sili atu i le 100, ma o le aofa'i o a'afiaga na o'o atu i le 500.
● Su'esu'ega fa'apitoa o bioinformatics: fa'asologa o galuega e aofia ai su'esu'ega a le SNP-trait association, tu'uina atu o se seti o kenera sui ma a latou fa'amatalaga galue tutusa.
●Au'au bioinformatics maualuga ma fa'ata'amiloga pu'upu'u: fa'atasi ai ma le poto masani i su'esu'ega genomics alualu i luma, o le 'au a le BMKGene e tu'uina atu fa'amatalaga au'ili'ili ma se taimi vave fa'aliliu.
●Lagolago ina ua uma ona faatau atu:O la matou tautinoga e faʻalautele atu i tua atu o le faʻamaeʻaina o galuega faʻatasi ma le 3-masina pe a maeʻa le faʻatau atu vaitaimi auaunaga. I le taimi lea, matou te ofoina atu le tulitatao o poloketi, fesoasoani faʻafitauli, ma Q&A sauniga e faʻafeiloaʻi ai soʻo se fesili e fesoʻotaʻi ma iʻuga.
Auaunaga faʻamatalaga ma manaʻoga
| Ituaiga fa'asologa | Fautuaina le faitau aofai o tagata | Fuafuaga fa'asologa | Nucleotide manaʻomia |
| Fa'asologa atoa o Genome | 200 faʻataʻitaʻiga | 10x | Fa'atonuga: ≥ 1 ng/ µL Aofa'i aofa'i≥ 30ng Fa'atapula'a pe leai se fa'aleagaina po'o le fa'aleagaina |
| Vaega Fa'apitoa-Locus Amplified Fragment (SLAF) | Fa'ailoga loloto: 10x Numera o fa'ailoga: <400 Mb: Ua fautuaina le WGS <1Gb: 100K pine 1Gb > 2Gb: 300K pine Max 500k pine | Fa'asa'o ≥ 5 ng/µL Aofaʻi aofaʻi ≥ 80 ng Nanodrop OD260/280=1.6-2.5 Agarose gel: leai pe faʻatapulaʻaina le faʻaleagaina poʻo le faʻaleagaina
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Filifiliga Mea
O ituaiga eseese, subspecies, landraces/genebanks/aiga fefiloi/sources vao
O ituaiga eseese, subspecies, landraces
Aiga afa-sib/aiga-sib-atoa/aiga vao
Auala o Galuega Au'aunaga
Fa'ata'ita'iga mamanu
Fa'ata'ita'iga tu'uina atu
RNA su'ega
Fausia faletusi
Fa'asologa
Iloiloga fa'amaumauga
Au'aunaga pe a uma ona fa'atau
E aofia ai le au'ili'iliga nei:
- Su'esu'ega fa'atasi a Genome-lautele: LM, LMM, EMMAX, FASTLMM fa'ata'ita'iga
- Fa'amatalaga aoga o kenera sui
Su'esu'ega fa'atasi a le SNP - Manhattan plot
Su'esu'ega fa'atasi a le SNP-uiga - QQ plot
Su'esu'e le aga'i i luma na fa'afaigofieina e le BMKGene's de GWAS auaunaga e ala i le aoina o fa'asalalauga fa'apitoa:
Lv, L. et al. (2023) 'Malamalamaga i le fa'avae fa'avae o le fa'apalepale o le ammonia i le razor clam Sinonovacula constricta e ala ile su'esu'ega fa'atasi a genome-lautele',Fa'ato'aga, 569, itulau. 739351. doi: 10.1016/J.AQUACULTURE.2023.739351.
Li, X. et al. (2022) 'Su'esu'ega tele-omics o le 398 foxtail millet accessions e fa'aalia ai le genomic regions e feso'ota'i ma fale, uiga metabolite, ma a'afiaga fa'ama'i',La'au Molecular, 15(8), itulau 1367–1383. doi: 10.1016/j.molp.2022.07.003.
Li, J. et al. (2022) 'Genome-Wide Association Mapping of Hulless Barely Phenotypes in Drought Environment',Tuaoi i La'au Saienisi, 13, itulau. 924892. doi: 10.3389/FPLS.2022.924892/BIBTEX.
Zhao, X. et al. (2021) 'GmST1, lea e fa'ailogaina ai le sulfotransferase, fa'ae'eina le tete'e atu i soya mosaic virus strains G2 ma G3',La'au, Cell & Siosiomaga, 44(8), itulau 2777–2792. doi: 10.1111/PCE.14066.
