La'au/Manu Fa'asologa o Genome Atoa

La'au/Manu Atoa Genome Sequencing Ata Fa'aalia

O le toe fa'asologa atoa o le genome, lea e ta'ua o le WGS, e mafai ai ona fa'aalia uma suiga masani ma seasea i luga o le genome atoa e aofia ai le Nucleotide Polymorphism (SNP), Fa'aofiina o le Deletion (InDel), Structure variation (SV), ma Copy Number Variation (CNV). ).O SV e fausia ai se vaega tele o le fesuiaiga faavae nai lo SNPs ma e sili atu lona aafiaga i le genome, lea e iai sona aafiaga taua i meaola ola.O le toe faitauina umi-faitau e mafai ai ona iloa atili sa'o o vaega tetele ma fesuiaiga faigata aua o le faitau umi e faigofie ai ona sopoia le chromosomal i luga o vaega faigata e pei o le toe faia o le tandem, GC/AT-rich regions, ma le hyper-variable regions.

Fa'avae: Illumina, PacBio, Nanopore

Faafesootai matou

Au'aunaga Fa'amatalaga

Fa'aaliga Fa'aaliga

Lomiga Fa'apitoa

1. Auaunaga Tulaga

Ole poto masani ile fa'asologa o genome mo le silia ma le 1000 ituaiga.

E silia ma le 800 mataupu fa'asalalau fa'atasi ai ma le fa'atupuina o a'afiaga e sili atu i le 4000.

Au'ili'iliga bioinformatics atoatoa ile vala'au eseese ma su'esu'ega galuega.

2. Auaunaga Fa'amatalaga

Fa'avae		Faletusi	Fautuaina seq loloto
Illumina		PE 150	Mo SNP, InDel valaau ≥ 10x Mo SV, CNY valaau ≥ 30x
Nanopore		8 kb	Mo SV, CNY valaau ≥ 20x
Pacbio	CCS	15 kb	Mo SNP, InDel, SV, CNY valaau ≥ 10x

3. Fa'ata'ita'iga Manaoga

Fa'avae		Conc.(ng/μL)	Aofa'i (ng)	Mama		Akaraose gel
				OD260/280	OD260/230	1. Fa'amama fa'aili autu e leai pe fa'atapula'ainafa'aleagaina ua matauina ile gel. 2. Leai po'o le fa'atapula'aina o le RNA po'o le palatini
Illumina		≥1	≥30	-	-
Nanopore		≥30	Fa'alagolago i fa'amaumauga e maua mai 10μg/selau	1.7-2.2	≥1.5
Pacbio	CCS	≥50		1.7-2.2	1.8-2.5

4. Bioinformation Iloiloga

5. Galulue Galuega Au'aunaga

Fa'ata'ita'iga tu'uina atu

Su'ega DNA

Fauga faletusi

Fa'asologa

Iloiloga fa'amaumauga

Tuuina atu o faʻamatalaga

Muamua: BMKMANU S1000 Spatial Transcriptome

Sosoo ai: Fa'asologa o le RNA nucleus e tasi

1) Fa'amaumauga o Fa'afanua Genome

Fuafuaga 1 Fa'amaumauga o fa'asologa o fa'afanua

Ata 1 Tufatufaina ole lapo'a fa'aofi ma faitau fa'asalalau.

2) Su'esu'ega Su'esu'ega

Ata 2 Fa'amaumauga ma fa'amatalaga o SNP/INDEL/SV i Fa'ata'ita'iga

Ata 3 Genome-lautele tufatufaina o vatiations

3) Fa'amatalaga fa'atino o fesuiaiga

2019	Fesootaiga faanatura	O le fa'asologa atoa o le genome e fa'aalia ai le tupuaga o Brassica napus ma le kenera loci o lo'o a'afia i lona fa'aleleia
2020	PNAS	O le amataga o le evolusione ma le tala faasolopito o le i'a auro (Carassius auratus)
2021	La'au Biotechnology Journal	Fa'asinoga genomes o ituaiga jute e lua ua totoina
2021	La'au Biotechnology Journal	Saini genomic o fualaau faisua ma suau'u allopolyploid Brassicajuncea ma genetic loci pulea le faaputuputuina o glucosinolates
2019	La'au mole	O le fa'asologa atoa o le genome o se aofa'iga i le lalolagi atoa o fa'asoa fa'atosina o lo'o fa'aalia ai le fa'avae fa'avae o lo latou 'ese'esega fa'anatura.
2022	Su'esu'ega Fa'ato'aga	Genome-wide association su'esu'ega e maua ai fa'amatalaga mole mole i le suiga fa'anatura ole lapo'a fatu meleni
2021	Journal of Experimental Botany	O suʻesuʻega faʻatasi a le Genome-lautele e faʻaalia ai fesuiaiga o le GhSAD1 e tuʻuina atu le faʻapalepale malulu ile cotton
2021	Journal of Experimental Botany	O suʻesuʻega a le Genome-wide ma le faʻatusatusaga o tusitusiga o loʻo faʻaalia ai tala fou QTL ma genes sui e pulea le lapopoa o le petal i le rapeseed.