● Sauniuniga faletusi e mafai ona fa'ata'atia pe leai se PCR
● E maua ile 4 fa'asologa fa'asologa: Illumina NovaSeq, MGI T7, Nanopore Promethion P48, po'o PacBio Revio.
● Bioinformatic suʻesuʻega e taulaʻi i le suʻeina o fesuiaiga: SNP, InDel, SV ma CNV
●Tomai Tele ma Faamaumauga Lomiga: O le fa'aputuina o le poto masani i le fa'asologaina o le genome mo le silia ma le 1000 ituaiga ua i'u ai i le silia ma le 1000 fa'asalalauga fa'asalalau fa'atasi ai ma le fa'atuputeleina o a'afiaga e sili atu i le 5000.
●Au'ili'iliga Bioinformatics: E aofia ai fesuiaiga o le valaau ma galuega fa'amatalaga.
● Lagolago ina ua uma ona faatau atu:O la matou tautinoga e faʻalautele atu i tua atu o le faʻamaeʻaina o galuega faʻatasi ma le 3-masina pe a maeʻa le faʻatau atu vaitaimi auaunaga. I le taimi lea, matou te ofoina atu le tulitatao o poloketi, fesoasoani faʻafitauli, ma Q&A sauniga e faʻafeiloaʻi ai soʻo se fesili e fesoʻotaʻi ma iʻuga.
●Fa'amatalaga Atoa: Matou te fa'aogaina le tele o fa'amaumauga e fa'ailoa ai genes ma suiga fa'aalia ma fa'atino le su'esu'ega fa'atamaoaigaina talafeagai, maua ai fa'amatalaga i le tele o galuega su'esu'e.
Vaaiga eseese e iloagofie | Fuafuaga fa'asologa | Fautuaina loloto |
SNP ma InDel | Illumina NovaSeq PE150 poʻo le MGI T7 | 10x |
SV ma le CNV (le sa'o) | 30x | |
SV ma CNV (sili atu sa'o) | Nanopore Prom P48 | 20x |
SNPs, Indels, SV ma CNV | PacBio Revio | 10x |
Tisu po'o su'esu'e nucleic acids | Illumina/MGI | Nanopore | PacBio
| ||
Manu Viscera | 0.5-1 g | ≥ 3.5 g
| ≥ 3.5 g
| ||
Musele Manu | ≥ 5 g
| ≥ 5 g
| |||
Toto Mamalia | 1.5 mL | ≥ 0.5 mL
| ≥ 5 mL
| ||
Toto moa/I'a | ≥ 0.1 mL
| ≥ 0.5 mL
| |||
La'au- Laulau fou | 1-2 g | ≥ 2 g
| ≥ 5 g
| ||
Selula Aganuu |
| ≥ 1x107
| ≥ 1x108
| ||
Iniseti aano vaivai/Taitoatasi | 0.5-1 g | ≥ 1 g
| ≥ 3 g
| ||
DNA aumai
| Fa'atonuga: ≥ 1 ng/ µL Aofaiga: ≥ 30 ng Fa'atapula'a pe leai se fa'aleagaina po'o le fa'aleagaina
| Fa'atotonuga Aofaiga
OD260/280
OD260/230
Fa'atapula'a pe leai se fa'aleagaina po'o le fa'aleagaina
| ≥ 40 ng/ µL 4 µg/fesosolo sela/fa'ata'ita'iga
1.7-2.2
≥1.5 | Fa'atotonuga Aofaiga
OD260/280
OD260/230
Fa'atapula'a pe leai se fa'aleagaina po'o le fa'aleagaina | ≥ 50 ng/ µL 10 µg/fela solo/fa'ata'ita'iga
1.7-2.2
1.8-2.5 |
Sauniuniga Faletusi e leai se PCR: Fa'atotonuga≥ 40 ng/ µL Aofaiga≥ 500 ng |
E aofia ai le au'ili'iliga nei:
Fuainumera o le fa'aogaina i fa'asinoga genome - fa'asologa o le loloto o le tufatufaina
SNP valaau i le tele o faʻataʻitaʻiga
Fa'ailoga InDel - fa'amaumauga o le umi o InDel i le itulagi CDS ma le genome-lautele itulagi
Fa'asoa eseese i le genome - Circos plot
Fa'amatalaga fa'atino o kenera fa'atasi ai ma suiga fa'ailoa - Gene Ontology
Chai, Q. et al. (2023) 'O le glutathione S-transferase GhTT19 e fuafua ai le fa'a'ofuina o laula'au fuga e ala i le fa'atonutonuina o le fa'aputuina o le anthocyanin i totonu o le cotton', Plant Biotechnology Journal, 21(2), itulau. 433. doi: 10.1111/PBI.13965.
Cheng, H. et al. (2023) 'Chromosome-level wild Hevea brasiliensis genome e maua ai meafaigaluega fou mo le fa'atupuina o le genomic-fesoasoani ma nofoaga taua e siitia ai fua pa'u', Plant Biotechnology Journal, 21(5), itulau 1058–1072. doi: 10.1111/PBI.14018.
Li, A. et al. (2021) 'Genome of the estuarine oyster e maua ai fa'amatalaga i aafiaga o le tau ma fetuutuuna'i palasitika', Communications Biology 2021 4:1, 4(1), itulau 1–12. doi: 10.1038/s42003-021-02823-6.
Zeng, T. et al. (2022) 'Su'esu'ega o suiga o le genome ma le methylation i moa fa'aSaina i le aluga o taimi e maua ai le fa'amatalaga i le fa'asaoina o ituaiga', Communications Biology, 5(1), itulau 1–12. doi: 10.1038/s42003-022-03907-7.