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Chromatin Immunoprecipitation Sequencing (ChIP-seq)
O le ChIP-Seq e tu'uina atu ai le fa'avasegaina o le genome-lautele o taula'iga DNA mo suiga fa'asolopito, fa'asologa o tusitusiga, ma isi polotini e feso'ota'i DNA.E tu'ufa'atasia le filifilia o le chromatin immuno-precipitation (ChIP) e toe fa'aleleia ai fa'alavelave fa'apolotini-DNA fa'apitoa, fa'atasi ai ma le mana o le fa'asologa o le isi augatupulaga (NGS) mo le fa'asologa maualuga o le DNA toe maua.E le gata i lea, talu ai o le protein-DNA lavelave e toe maua mai sela ola, e mafai ona faʻatusatusa nofoaga o loʻo fusifusia i ituaiga sela eseese ma aano, pe i lalo o tulaga eseese.O talosaga e amata mai i tulafono faatonutonu tusitusia i auala tau atina'e i faiga fa'ama'i ma tua atu.
Tulaga: Illumina NovaSeq Platform
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Fa'asologa atoa o le genome bisulfite
DNA methylation i le tulaga lona lima i le cytosine (5-mC) o loʻo i ai se aʻafiaga taua i le faʻaalia o le kenera ma gaioiga feaveaʻi.O faʻasologa o le methylation e le masani ai ua fesoʻotaʻi ma le tele o tulaga ma faʻamaʻi, e pei o le kanesa.O le WGBS ua avea ma tulaga auro mo le suʻesuʻeina o le genome-wide methylation ile faʻavae tasi.
Tulaga: Illumina NovaSeq Platform
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Su'esu'ega mo le Transposase-Accessible Chromatin fa'atasi ai ma le fa'asologa maualuga (ATAC-seq)
ATAC-seq o se metotia faʻasolosolo maualuga mo le suʻesuʻeina o le genome-wide chromatin accessibility, lea e taua mo le pulea epigenetic lalolagi o faʻamatalaga o kenera.E fa'apipi'i fa'asologa fa'asologa i vaega o chromatin matala e ala ile Tn5 transposase.A maeʻa le PCR faʻateleina, e fausia se faletusi faʻasologa.O vaega uma o le chromatin e mafai ona maua i lalo o se tulaga avanoa-taimi fa'apitoa, e le gata i nofoaga o lo'o fusifusia ai se vaega fa'aliliuga, po'o se vaega fa'asologa fa'asolosolo.
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Fa'aitiitiga Fa'atusa o le Bisulfite Sequencing (RRBS)
Ole su'esu'ega ole DNA methylation e masani lava ona avea ma autu vevela ile su'esu'ega fa'ama'i, ma e feso'ota'i vavalalata ma fa'amatalaga o kenera ma uiga fa'apitoa.RRBS o se auala saʻo, lelei ma taugofie mo suʻesuʻega DNA methylation.Fa'atamaoaigaina o le fa'alauiloaina ma le CpG motu motu e ala i le enzymatic cleavage (Msp I), fa'atasi ma le fa'asologa o le Bisulfite, e maua ai le su'esu'ega o le DNA methylation maualuga.
Tulaga: Illumina NovaSeq Platform
