Prokaryotic RNA fa'asologa

Prokaryotic RNA sequencing e fa'aogaina ai le fa'asologa o augatupulaga (NGS) e fa'aalia ai le i ai ma le aofa'i o le RNA i se taimi, e ala i le su'esu'eina o le suiga o le telefoni feavea'i.O le fa'asologa o le RNA prokaryotic a la matou kamupani, e fa'atatau tonu lava i prokaryotes ma genomes fa'asinomaga, tu'uina atu ia te oe fa'amatalaga fa'aliliuga, su'esu'ega fa'asologa o gene, ma isi. Ua fa'aaogaina lautele i su'esu'ega fa'asaienisi fa'avae, su'esu'ega o vaila'au ma atina'e, ma isi mea.

Tulaga: Illumina NovaSeq Platform

Faafesootai matou

Au'aunaga Fa'amatalaga

Fa'amatalaga Auaunaga

Tulaga: Illumina NovaSeq tulaga, PE150

Ituaiga faletusi: 350bp fa'aofi le faletusi cDNA (fa'alagolago ile fa'asoa maualuga)

Fuafuaga fa'asologa: Fa'atasi-Fa'ai'uga 150 bp

Fautuaina le aofaʻi o faʻamatalaga: 2 Gb raw data / faʻataʻitaʻiga

Bioinformatics

(1) Fa'asologa o fa'amaumauga o fa'atonuga lelei: Tu'ufa'atasiga Nucleotide, Fa'asoa tulaga lelei, su'esu'ega rRNA;

(2) Su'esu'ega o le fa'asologa o fa'asologa: Fa'amaumauga o fa'amaumauga o le fa'asologa, Tulaga o le fa'asologa, Kava o le fa'asologa;

(3) Faʻamatalaga faʻamatalaga genome (NR, Swiss-Prot, Pfam, COG, GO, KEGG);

(4) Faʻamatalaga faʻamatalaga: Faʻasalalauga faʻamatalaga (Faʻatasi ma le lua pe sili atu faʻataʻitaʻiga), Iloiloga o faʻamatalaga i le va o faʻataʻitaʻiga (Venn suʻesuʻega, suʻesuʻega Faʻatasi, suʻesuʻega PCA);

(5) Su'esu'ega fa'aaliga eseese (Fa'atasi ma fa'ata'ita'iga se lua pe sili atu);

(6) Su'esu'ega fa'asologa o Gene: Su'esu'ega Venn, Su'esu'ega Cluster, Su'esu'ega fa'amatalaga galuega (COG, GO, KEGG), Su'esu'ega fa'atamaoaigaina o galuega (GO, KEGG), Kalafi fa'atamaoaigaina galuega, su'esu'ega Ipath;

(7) su'esu'ega sRNA: va'aiga sRNA, fa'amatalaga sRNA (Blast, sRNAMap, sRNATarBase, SIPHT, Rfam), sRNA fausaga lona lua va'aiga, sRNA taula'iga gene va'aiga;

(8) Suʻesuʻega faʻasologa o tusitusiga: Operon suʻesuʻega, Faʻamatalaga amata ma faʻaiʻuga nofoaga vavalo, faʻamatalaga UTR ma auʻiliʻiliga, Faʻasalalauga faʻasologa o faʻasalalauga, suʻesuʻega SNP / InDel (SNP / InDel faʻamatalaga, faʻasalalauga faʻaitulagi SNP / InDel, fuainumera SNP).