Prokaryotic rna sequering
Foliga
● Rna faʻataʻitaʻiga gaosi e aofia ai rrna uppleation na sosoo ai e Stail RNA Fale Tusi Lesona.
● Agoninictatic auiliiliga faʻavae e faʻavae i luga o le ogatasi i se faʻasino manatu
● O le auiliiliga e aofia ai le Geneware Faʻaupuga ma DEGS ae o loʻo faʻamatala mai foi le faʻamatalaga
Tulaga lelei
●Malo le lelei: Matou te faʻatinoina vaega o loʻo faʻaaogaina i vaega uma, mai le faʻataʻitaʻiga ma le faletusi tapenaga i se lipine ma bioninictoctics. O lenei manatu faʻapitoa e mautinoa ai le tuʻuina atu o le sili ona lelei-tulaga maualuga.
●Paga-faʻapitoa faʻasologa o faʻamatalaga: Ona o Sauniga o Ruma
●Faauma auiliiliga fetuutuunai i prokaryotic Transporttomes: O le paionegotamiga paipa o loʻo aofia ai le naʻo le auiliiliga o le paluga, e aofia ai foʻi ma le auiliiliga o le fausaga tusitusi, ma sui o tagata. E aofia ai foʻi le auiliiliga o le malae otaota, ua taʻua ai ma le vavalo o tulaga lua o loʻo ma sini.
●Meli-faatau lagolago: O la tatou tautinoga na sosoʻo atu i tua atu o le poloketi ua maeʻa le 3-masina ina ua faʻatau atu auaunaga. I le taimi lea, matou te ofoina atu ai le faʻagaioiga e mulimulitaʻia, faʻafitauli fesoasoani, ma Q & ma ni sauniga e faʻailoa ai soʻo se fesili e fesoʻotaʻi ma faʻaiuga.
Faataʻitaʻi Manaʻoga ma Tuʻuina atu
| Faletusi | SUʻESUʻEGA FAʻATASI | Faamatalaga Fautuaina | Lelei Puleaina |
| Na faaitiitia RRNA | Imumlina pex50 | 1-2 GB | Q30085% |
Faʻataʻitaʻiga Manaʻoga:
| Pepa. (Ng / μl) | Aofaiga (μg) | Amio mama | Sao |
| ≥ 50 | ≥ 1 | OD260 / 280 = 1.8-2.0 Od260 / 230 = 1.0-2.5 Faʻatapulaʻa pe leai se porotini poʻo le DNA o loʻo faʻaalia o le gel. | RINGOW6.5 |
Fautuaina le Aiatusi Tuufaatasi
Container: 2 ml centrifrifoge tube (tin fortil e le fautuaina)
Faataʻitaʻi igoa: Vaega + Remactate eg A1, A2, A3; B1, b2, b3.
Uta:
1. Meaola-Ice: faʻataʻitaʻiga e tatau ona teu i ato ma tanu i le mama-aisa.
2. Rnastable tubes: rna faʻataʻitaʻiga e mafai ona mago i le rna stabilization tube (eg rnastable®) ma lafo i le vevela vevela.
Auaunaga galue
Faataʻitaʻiga Faafou
Fausiaina o le Faletusi
Liu
Faamatalaga o faamatalaga
A maeʻa-faʻatau auaunaga
Talaaopio Iloilo auiliiliga galuega
E aofia ai le auiliiliga lenei:
● Vave faʻamatalaga lelei
● Ogatasi ma le manatu i le manatu
● Tusitala le Iloiloga Agavaa: RNA Fracmentation Sociance, Faʻaofi le tele ma le le mautonu ma le le mautonu
● Faʻatinoina o le faʻamatalaga o le vavalo
● FAʻAALIGA FAʻAALIGA: FESOOTAIGA MA MANUIA MA LE FAʻAFETAI FAʻAMATALAGA (PCA)
● Tetee Gene Faasilasilaga (DEGS)
● O le faʻamatalaga o le faʻamatalaga ma le faʻauiga o le degs
● Surna Suʻega: Vaeluaina, Faʻafanua, Sini, ma lona lua fausaga o le taimi nei
● O faʻamatalaga o loʻo faʻaogaina: Operons, amata ma le faʻaiʻuga tulaga, e le mafai ona faʻatagaina le itulagi (UTS), faʻalauiloa, ma le SNEP / UNGR
Sequening Sauration
Actiilil seleni o le coding gene
Fesoʻotaʻiga i le va o faʻataʻitaʻiga
Eseesega faaalia Genera (DEGS) Iloiloga
Iloiloga Faʻamaoniga Faʻapitoa
SRNNA FAAPEFEA
Saili i alualu i luma faʻalauteleina e le BMKGENA's Nanopore atoa-umi-umi le umi o le DRA o le DRAN Conquetion Service Services i lenei faʻasalalauga lomiga.
Guan, CPt et al. (2018) 'O le Global Transcrictome suiga o BioFilm-fausia Staphylococcus epidermicIDIDis tali atu i le atoa alkoids o Sopholoa.PILES COUDS ORRIBIYUY, 67 (2), i. 223. DOI: 10.2130307 / PJM-2019-024.
