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Oloa

Laititi RNA fa'asologa-Illumina

Laiti RNA (sRNA) molelaʻau, e masani lava i lalo ole 200 nucleotides le umi, e aofia ai microRNAs (miRNAs), RNAs faʻalavelave laiti (siRNAs), ma piwi-interacting RNAs (piRNAs).Faatasi ai ma nei mea, miRNAs, e tusa ma le 20-24 nucleotides le umi, e sili ona mataʻina mo a latou matafaioi faʻatulafonoina i le tele o gaioiga feaveaʻi.Fa'atasi ai ma fa'ata'ita'iga fa'amatalaga fa'apitoa ma tulaga fa'apitoa, fa'aalia e miRNA le fa'asao maualuga i ituaiga eseese.

Fa'avae: Illumina NovaSeq


Au'aunaga Fa'amatalaga

Bioinformatics

I'uga Fa'aaliga

Lomiga Fa'apitoa

Vaega

● Filifiliga tele ole RNA a'o le'i saunia le faletusi

● Bioinformatic su'esu'ega e fa'atotonugalemu ile va'aiga o le miRNA ma a latou fa'amoemoe

Tulaga Auaunaga

Au'ili'iliga bioinformatics atoatoa:mafai ai le faʻamaoniaina o miRNAs lauiloa ma tala fou, faʻamaonia o sini faʻatatau miRNA ma faʻamatalaga galue tutusa ma faʻatamaoaigaina i le tele o faʻamaumauga (KEGG, GO)

Pulea lelei: matou te fa'atinoina le fa'atonuga autu i la'asaga uma, mai le fa'ata'ita'iga ma tapenaga faletusi i le fa'asologa ma bioinformatics.O lenei mata'ituina lelei e fa'amautinoa ai le tu'uina atu o fa'ai'uga maualuluga.

Lagolago Post-Fa'atau: O la matou tautinoga e faʻalautele atu i tua atu o le faʻamaeʻaina o galuega faʻatasi ma le 3-masina pe a maeʻa le faʻatau atu taimi o auaunaga.I le taimi lea, matou te ofoina atu le tulitatao o poloketi, fesoasoani faʻafitauli, ma Q&A sauniga e faʻafeiloaʻi ai soʻo se fesili e fesoʻotaʻi ma iʻuga.

Tomai Tele: fa'atasi ai ma se fa'amaumauga o le tapunia manuia o le tele o poloketi sRNA e aofia ai le silia ma le 100 ituaiga i vaega su'esu'e eseese, o la matou 'au e aumaia le tele o le poto masani i galuega uma.

Fa'ata'ita'iga Mana'oga ma le Tu'uina atu

Faletusi

Fa'avae

Fa'amatalaga fautuaina

Fa'amatalaga QC

Tele ua filifilia

Illumina SE50

10M-20M faitau

Q30≥85%

Fa'ata'ita'iga Mana'oga:

Nucleotides:

Conc.(ng/μl)

Aofa'i (μg)

mama

Fa'amaoni

≥ 80

≥ 0.5

OD260/280=1.7-2.5

OD260/230=0.5-2.5

Fa'atapula'a pe leai se polotini po'o le fa'aleagaina o DNA o lo'o fa'aalia ile gel.

RIN≥6.5;

5.0≥28S/18S≥1.0;

fa'atapula'a pe leai se maualuga maualuga

● La'au:

A'a, Po'o Po'o Petal: 450 mg

Laulaau po'o Fatu: 300 mg

Fualaau: 1.2 g

● Manu:

Loto po'o le Intestinal: 450 mg

Viscera poʻo Faiʻai: 240 mg

Muso: 600 mg

Ivi, Laulu po'o Pa'u: 1.5g

● Arthropods:

Iniseti: 9g

Crustacea: 450 mg

● toto atoa: 2 paipa

● Selula: 106 sela

● Serum ma Plasma:6 mL

Fautuaina Fa'ata'ita'iga Tu'uina atu

pusa:
2 ml centrifuge paipa (E le fautuaina le pepa apa)
Fa'ailoga fa'ata'ita'iga: Vaega+fa'atusa eg A1, A2, A3;B1, B2, B3... ...

utaina:
1. Dry-aisa: Fa'ata'ita'iga e mana'omia ona fa'apipi'i i taga ma tanu i le aisa-mago.
2.RNAstable tubes: RNA samples e mafai ona faʻamago i le RNA stabilization tube(eg RNAstable®) ma lafo i le vevela potu.

Auala o Galuega Au'aunaga

Fa'ata'ita'iga QC

Fa'ata'ita'iga mamanu

fa'ata'ita'iga tu'uina atu

Fa'ata'ita'iga tu'uina atu

Fa'ata'ita'iga pailate

RNA su'ega

Sauniuniga Faletusi

Fausia faletusi

Fa'asologa

Fa'asologa

Iloiloga fa'amaumauga

Iloiloga fa'amaumauga

A uma le fa'atauga Auaunaga

Au'aunaga pe a uma ona fa'atau


  • Muamua:
  • Sosoo ai:

  • Bioinformatics

    wps_doc_14

    Faailoaina o le miRNA: fausaga ma le loloto

     

     

     miRNA-precursor-structure-ma-sequencing-loloto

     

    Fa'aaliga 'ese'ese o le miRNA - fa'apipi'i fa'apitoa

    图片34

     

    Fa'amatalaga fa'atino o le fa'amoemoe o miRNA fa'aalia fa'aalia

    图片35

    Su'esu'e le alualu i luma o su'esu'ega na fa'afaigofieina e le BMKGene' sRNA sequencing services e ala i le aoina o fa'asalalauga.

      

    Chen, H. et al.(2023) 'O fa'ama'i fa'ama'i e taofia ai le biosynthesis saponin ma le photosynthesis i Panax notoginseng', Plant Physiology and Biochemistry, 203, i.108038. doi: 10.1016/J.PLAPHY.2023.108038.

    Li, H. et al.(2023) 'O le laau FYVE domain-containing protein FREE1 e fesoʻotaʻi ma vaega microprocessor e taofiofia le miRNA biogenesis', EMBO lipoti, 24(1).doi: 10.15252/EMBR.202255037/SUPPL_FILE/EMBR202255037-SUP-0004-SDATAFIG4.TIF.

    Yu, J. et al.(2023) 'O le MicroRNA Ame-Bantam-3p Pulea le Atina'eina o Pupal Larval e ala i le Fa'aautuina o le Fa'atupuina Fa'atupu Fa'atupu Fa'a-pei o Domains 8 Gene (megf8) i le Honeybee, Apis mellifera', International Journal of Molecular Sciences, 24(6), p .5726. doi: 10.3390/IJMS24065726/S1.

    Zhang, M. et al.(2018) 'Suʻesuʻega Tuʻufaʻatasia o MiRNA ma Genes e fesoʻotaʻi ma Aano o manufasi e faʻaalia ai o le Gga-MiR-140-5p e aʻafia ai le faʻaogaina o le gaʻo i totonu o moa', Cellular Physiology and Biochemistry, 46 (6), itulau 2421-2433.doi: 10.1159/000489649.

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