Fa'asologa atoa o le genome bisulfite
Tulaga Auaunaga
● Fa'auma atoatoa: tu'uina atu auaunaga sili ona lelei e tasi mai le fa'ata'ita'iga fa'ata'ita'iga, fau faletusi, fa'asologa i su'esu'ega bioinformatics.
● Saʻo maualuga: Mature methylation faʻaliliuina tekinolosi e mafai ona suʻesuʻeina saʻo le tulaga methylation o se tasi C faavae.
● Fa'asalalau lautele: su'esu'eina o nofoaga o le methylation ile genome-lautele tulaga.
● Toe faʻaleleia lelei: faʻatautaia mautu, talafeagai mo suʻesuʻega faʻatusatusa i le tele o faʻataʻitaʻiga.
Fa'ata'ita'iga Fa'amatalaga
|
Fa'avae
|
Faitau Umi
|
Fuafuaga Fa'asologa
|
| Illumina | PE 150 | 30X, tolu fa'asologa o meaola, fa'atasi ai ma genome fa'amatalaga maualuga |
Fa'ata'ita'iga Manaoga
|
Ituaiga Fa'ata'ita'i
|
Aofa'i (μg)
|
Conc.(ng/μl)
|
Volume (μl)
|
mama
|
| gDNA | ≥ 2 | ≥ 20
| ≥ 20
| Fa'atapula'aina le fa'aleagaina ma le fa'aleagaina
|
Auala o Galuega Au'aunaga
Fa'ata'ita'iga tu'uina atu
Su'ega DNA
Fausia faletusi
Fa'asologa
Iloiloga fa'amaumauga
Tuuina atu o faʻamatalaga
Bioinformatics
1. Genome-lautele DNA methylation tufatufaina
2.Faʻamatalaga i luga ole itulagi CGI maualuga-methylated
