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10x Genomics Visium Spatial Transcriptome

Spatial transcriptomics tekinoroji yekucheka-kumucheto inobvumira vaongorori kuti vaongorore maitiro ekutaura kwemajini mukati mematishu vachichengetedza mamiriro avo epamhepo.Imwe puratifomu ine simba mune ino domain ndeye 10x Genomics Visium yakasanganiswa neIllumina kutevedzana.Iyo musimboti we10X Visium iri pane yakasarudzika chip ine yakasarudzika nzvimbo yekubata inoiswa zvikamu zvetishu.Iyi nzvimbo yekubata ine barcoded mavara, imwe neimwe inoenderana nenzvimbo yakasarudzika mukati mematishu.Iwo akabatwa maRNA mamorekuru kubva munyama anobva anyorwa neakasarudzika mamorekuru identifiers (UMIs) panguva yekudzokesa kunyorwa maitiro.Aya mabarcoded spots uye UMIs anogonesa chaiyo spatial mepu uye quantification yegeneti kutaura pane imwechete-sero resolution.Iko kusanganiswa kwe spatially barcoded samples uye UMIs inovimbisa kurongeka uye kujeka kweiyo data inogadzirwa.Nekushandisa iyi Spatial Transcriptomics tekinoroji, vaongorori vanogona kuwana nzwisiso yakadzama yekurongeka kwenzvimbo yemasero uye kupindirana kwakaoma kwema molecular kunoitika mukati mematishu, zvichipa ruzivo rwakakosha mumatanho ari pasi pemaitiro ezvipenyu mundima dzakawanda, kusanganisira oncology, neuroscience, developmental biology, immunology. , uye zvidzidzo zvebotanical.

Platform: 10X Genomics Visium uye Illumina NovaSeq


  • FOB Mutengo:US $0.5 - 9,999 / Piece
  • Min.Order Quantity:100 Piece/Zvimedu
  • Supply Ability:10000 Piece / Zvimedu pamwedzi
  • Service Details

    Bioinformatics

    Demo Results

    Zvinyorwa zvinoratidzwa

    Features

    ● Kugadziriswa: 100 µM

    ● Spot Diameter: 55 µM

    ● Nhamba yenzvimbo: 4992

    ● Kutora nzvimbo: 6.5 x 6.5 mm

    ● Nzvimbo yega yega ine barcode yakatakurwa neprimers inoumbwa nezvikamu zvina:

    - poly(dT) muswe wemRNA priming uye cDNA synthesis

    -Unique Molecular Identifier (UMI) kugadzirisa kusarura kwekusimudzira

    - Spatial barcode

    -Kusunga kutevedzana kwechidimbu kuverenga 1 kutevedzana kwekutanga

    ● H & E kusvibiswa kwezvikamu

    Zvakanakira

    One-stop service: inobatanidza zvese ruzivo uye hunyanzvi-hwakavakirwa matanho, kusanganisira cryo-chikamu, kusvibisa, optimization yetishu, spatial barcoding, kugadzirira raibhurari, kutevedzana uye bioinformatics.

    ● Chikwata chehunyanzvi chehunyanzvi: ine ruzivo mumhando dzinopfuura mazana maviri nemakumi mashanu emhando dzematishu uye 100+ mhando dzinosanganisira vanhu, mbeva, zvinoyamwisa, hove uye zvidyarwa.

    Real-time update pane yese purojekiti: nekutonga kwakazara kwekufambira mberi kwekuedza.

    Yakakwana yakajairika bioinformatics:pasuru inosanganisira 29 ongororo uye 100+ yepamusoro-mhando nhamba.

    Customized data kuongorora uye kuona: inowanikwa kune zvakasiyana zvikumbiro zvekutsvaga.

    Optional joint analysis neSingle-cell mRNA sequencing

    Zvinotsanangurwa

    Mienzaniso Zvinodiwa

    Library

    Sequencing strategy

    Data inokurudzirwa

    Quality Control

    OCT-yakaiswa cryo samples, FFPE samples

    (Yakanyanya kureba: approx. 6x6x6 mm3)

    3 zvidhinha pamuenzaniso

    10X Visium cDNA raibhurari

    Illumina PE150

    50K PE inoverenga pane imwe nzvimbo

    ( 60Gb )

    RIN>7

    Kuti uwane rumwe ruzivo nezve nhungamiro yegadziriro yemuenzaniso uye mafambiro ebasa, ndapota inzwa wakasununguka kutaura kune a

    Service Workflow

    Muchikamu chekugadzirira chemuenzaniso, yekutanga kuwanda kweRNA kutorwa kuyedzwa kunoitwa kuti ive nechokwadi chekuti RNA yemhando yepamusoro inogona kuwanikwa.Muchikamu chekugadzirisa matishu zvikamu zvakasvibiswa uye zvinoonekwa uye mamiriro epermeabilization ekuburitswa kwemRNA kubva munyama anogadziriswa.Iyo yakagadziridzwa protocol inozoshandiswa panguva yekuvaka raibhurari, ichiteverwa nekutevedzana uye kuongororwa kwedata.

    Iyo yakazara sevhisi kufambiswa kwebasa kunosanganisira chaiyo-nguva yekuvandudza uye kusimbiswa kwevatengi kuchengetedza inopindura mhinduro loop, kuve nechokwadi chekuita kwakatsetseka kweprojekiti.

    图片4

  • Zvakapfuura:
  • Zvinotevera:

  • 10x (9)

     

    Inosanganisira ongororo inotevera:

     Data Quality Control:

    o Kubuda kwedata uye kugovera zvibodzwa zvemhando

    o Kuonekwa kwemajini pane imwe nzvimbo

    o Kuvharwa kwenyama

     Yemukati-sample wongororo:

    o Gene kupfuma

    o Spot clustering, kusanganisira yakaderedzwa dimension analysis

    o Kuongororwa kwekutaura kwakasiyana pakati pemasumbu: kuzivikanwa kwemakina magene

    o Inoshanda tsinhiro uye hupfumi hwemaka gene

     Inter-group analysis

    o Kubatanidzwazve kwemavara kubva kune ese masampuli (semuenzaniso. ane chirwere uye kutonga) uye kubatanidza zvakare

    o Kuzivikanwa kwemajini emakaki kusumbu rega rega

    o Inoshanda tsinhiro uye hupfumi hwemaka gene

    o Musiyano Kuratidzwa kwesumbu rimwechete pakati pemapoka

    Inner-sample Analysis

    Spot clustering

    10x (10)

     

    Mucherechedzo wemajini uye kugovera kwenzvimbo

     

    10x (12)

    10x (11)

     

    Inter-group Analysis

    Musanganiswa wedata kubva kumapoka maviri uye re-cluster

    10x (13)

     

     

    Marker majini emasumbu matsva

    图片5

    Ongorora kufambira mberi kwakafambiswa neBMKGene's spatial transcriptomics sevhisi ne10X Visium Mune aya akanyorwa zvinyorwa:

    Chen, D. et al.(2023) 'mthl1, inogona Drosophila homologue ye mammalian adhesion GPCRs, inobatanidzwa mukuita antitumor kune jekiseni oncogenic masero munhunzi', Proceedings of the National Academy of Sciences yeUnited States of America, 120 (30), p.e2303462120.doi: /10.1073/pnas.2303462120

    Chen, Y. et al.(2023) 'STEEL inogonesa yakakwirira-resolution delineation yespatiotemporal transcriptomic data', Pfupiso muBioinformatics, 24 (2), mapeji 1-10.doi: 10.1093/BIB/BBAD068.

    Liu, C. nevamwe.(2022) 'Aspatiotemporal atlas ye organogenesis mukugadzirwa kwemaruva eorchid', Nucleic Acids Research, 50 (17), mapeji 9724-9737.doi: 10.1093/NAR/GKAC773.

    Wang, J. nevamwe.(2023) 'Kubatanidza Spatial Transcriptomics uye Single-nucleus RNA Sequencing Inozivisa Ingangove Yekurapa Matanho eUterine Leiomyoma', International Journal yeBiological Sayenzi, 19 (8), mapeji 2515-2530.doi: 10.7150/IJBS.83510.

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