BMKCloud Log in
条形banner-03

Products

16S/18S/ITS Amplicon Sequencing-PacBio

Iyo subunit pa16S uye 18S rRNA ine ese akachengetedzeka zvakanyanya uye hyper-anochinjika matunhu ndeye yakakwana molecular zvigunwe zvekuzivikanwa kweprokaryotic uye eukaryotic zvipenyu.Tichitora mukana wekutevedzana, aya amplicon anogona kunangwa zvichibva pazvikamu zvakachengetwa uye matunhu anochinja-chinja anogona kunyatsoratidzwa hutachiona huchibatsira muzvidzidzo zvinofukidza hutachiona hwekusiyana kwekuongorora, taxonomy, phylogeny, nezvimwe. Imwe-morekuru real-time(SMRT) ) kutevedzana kwePacBio papuratifomu inogonesa kuwana kuverenga kwakareba kwakaringana, uko kunogona kuvhara maamplicon akazara (approx. 1.5 KB).Maonero akatambanudzwa enzvimbo yemajini akasimudzira zvakanyanya kugadziriswa kwezvipenyu mumutsara wemabhakitiriya kana fungi.

Platform:PacBio Sequel II


Service Details

Demo Results

Nyaya Yokudzidza

Basa Rinobatsira

1

● Kuverenga kwenguva refu kunoratidza zvizere-kureba kutevedzana kwe16S/18S/ITS

● Yakanyanya kurongeka base kufona nePacBio CCS mode sequencing

● Kugadziriswa kwemhando yemhando muOTU/ASV chirevo

● Yazvino QIIME2 inoongorora kuyerera ine ongororo dzakasiyana maererano nedatabase, zvirevo, OTU/ASV.

● Inoshanda kuzvidzidzo zvakasiyana-siyana zvenharaunda

● BMK ine ruzivo rwakakura neinopfuura 100,000 samples / gore, inovhara ivhu, mvura, gasi, sludge, tsvina, ura, ganda, kuvirisa muto, tumbuyu, zvirimwa, nezvimwe.

● BMKCloud yakagonesa kududzira dhata ine makumi mana neshanu emunhu ekuongorora maturusi

Sevhisi Zvinotsanangurwa

SequencingPlatform

Library

Yakakurudzirwa data

Nguva yekuchinja

PacBio Sequel II

SMRT-bhero

5K/10K/20K Tags

44 Mazuva ekushanda

Bioinformatics inoongorora

● Raw data quality control

● OTU clustering/De-noise(ASV)

● OTU chirevo

● Alpha kusiyana-siyana

● Beta siyana

● Kuongorora kweboka

● Kuongororwa kwesangano kunopesana nekuedza zvinhu

● Basa rekufanotaura kwemajini

16sPacbio

Sample Zvinodiwa uye Delivery

Sample Zvinodiwa:

ForDNA zvinyorwa:

Muenzaniso Type

Mari

Concentration

Kuchena

DNA zvinyorwa

> 1 μg

> 20 ng/μl

OD260/280= 1.6-2.5

Zvezvakatipoteredza samples:

Muenzaniso mhando

Inokurudzirwa sampling maitiro

Ivhu

Sampling huwandu: approx.5 g;Chinhu chakasara chakasvava chinoda kubviswa pamusoro;Geda zvidimbu zvakakura uye upfuure ne2 mm sefa;Aliquot samples mune sterile EP-chubhu kana cyrotube yekuchengetera.

Feces

Sampling huwandu: approx.5 g;Unganidza uye aliquot samples mune isinga bereki EP-tube kana cryotube yekuchengetera.

Intestinal contents

Samples dzinofanirwa kugadziriswa pasi peaseptic mamiriro.Geza matishu akaunganidzwa nePBS;Centrifuge iyo PBS uye tora iyo precipitant mu EP-machubhu.

Sludge

Sampling huwandu: approx.5 g;Unganidza uye aliquot sludge sampuli mune isina utachiona EP-tube kana cryotube yekuchengetera

Mvura

Kune sampuli ine hushoma huwandu hwehutachiona, hwakadai semvura yemupombi, mvura yemugodhi, nezvimwewo, Unganidza ingangoita 1 L mvura uye upfuure nepakati pe0.22 μm sefa yekupfumisa hutachiona pa membrane.Chengetedza membrane muchubhu isina utachiona.

Ganda

Nyatsokweva pamusoro peganda nemucheka wedonje usina utachiona kana blade yekuvhiya woiisa muchubhu isina utachiona.

Yakakurudzirwa Sample Delivery

Svitsa masampula munitrogen yemvura kwemaawa 3-4 uye chengetedza mune yemvura nitrogen kana -80 dhigirii kusvika kurebesa kwenguva refu.Sample shipping with dry-ice inodiwa.

Kuyerera Kwebasa Rebasa

sampuli yekuendesa

Muenzaniso wekutumira

Kugadzirira Raibhurari

Kuvakwa kweraibhurari

Sequencing

Sequencing

Data analysis

Data analysis

Mushure mekutengesa Services

Mushure mekutengesa masevhisi


  • Zvakapfuura:
  • Zvinotevera:

  • 1.Annotation rate yeV3 + V4 (Illumina)-based microbial community profiling vs. Full-length (PacBio)-based profiling.
    (Data remapurojekiti makumi matatu akatorwa zvisina tsarukano akaiswa pahuwandu)

    3

    2.Annotation mwero weakazara-kureba amplicon sequencing pamhando-nhanho mumhando dzakasiyana dzemuenzaniso

    4

    3.Kugovera marudzi

    5
    4.Phylogenetic muti

    6

    Nyaya yeBMK

    Arsenic exposure inoita kuti intestinal barriers ikuvadze uye zvinokonzeresa activation yegut-chiropa axis inotungamira mukuzvimba uye pyroptosis yechiropa mumadhadha.

    Rakatsikiswa:Sayenzi Yenzvimbo Yese,2021

    Sequencing strategy:

    Samples: Kudzora vs 8 mg / kg ATO yakafumurwa boka
    Sequencing data goho: 102,583 mbishi CCS sequences muhuwandu
    Kudzora: 54,518 ± 747 inoshanda CCS
    ATO-yakafumurwa : 45,050 ± 1675 inoshanda CCS

    Mibairo yakakosha

    Alpha kusiyana:Kuratidzwa kweATO kwakachinja zvakanyanya hupfumi hwemukati memukati uye kusiyana mumadhadha.

    Metastats kuongororwa:
    Mune phylum level: 2 bhakitiriya phyla inongowanikwa mumapoka ekutonga
    Mune genus level: 6 genera yakawanikwa yakasiyana zvakanyanya muhuwandu hwakawanda
    Muchikamu chezvipenyu: 36 marudzi akaonekwa akazara, uye 6 azvo akasiyana zvakanyanya mukuwanda kwerelavive.

    Reference

    Thingholm, LB, et al."Vanhu Vakafutisa vane uye vasina Type 2 Diabetes Vanoratidza Yakasiyana Gut Microbial Functional Capacity and Composition."Cell Host & Microbe26.2(2019).

    kuwana quote

    Nyora meseji yako pano uye titumire kwatiri

    Tumira meseji yako kwatiri: