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BMKMANU S1000 Spatial Transcriptome

Kurongeka kwenzvimbo yemaseru kunoita basa rakakosha mumaitiro akasiyana-siyana ebhayoloji, sekupinzwa kwemuviri, kukura kwe embryo, zvichingodaro. Spatial transcriptome sequencing, iyo inoratidza gene expression profiling uchichengeta ruzivo rwechinzvimbo, inopa ruzivo rwakakura mu transcriptome-level tissue architecture.Nekuvandudza tekinoroji, iyo yekupedzisira-yakajeka tishu morphology uye chaiyo dhizaini musiyano we spatial molecular expression inoda kudzidzwa nepamusoro resolution.BMKGENE inopa yakazara, imwe-stop spatial transcriptome sequencing sevhisi kubva kumasample kuenda kune biological ruzivo.

Spatial transcriptomics tekinoroji inogonesa maonero matsva munhandare yekutsvagisa dzakasiyana nekugadzirisa gene expression profil ine spatial yemukati mune heterogenous samples.

Spatial transcriptome chip: BMKMANU S1000

Platform: Illumina NovaSeq Platform


Service Details

Bioinformatics

Demo Results

BMKMANU S1000 Spatial Transcriptome Technical Scheme

S1000.

Zvakanakira zveBMKMANU S1000

1) Sub-cellular resolution: Nzvimbo yega yega yekutora yaive > 2 miriyoni spatial Barcoded Spots ine dhayamita ye2.5 µm uye nzvimbo ye5 µm pakati penzvimbo dzemavara, ichigonesa spatial transcriptome kuongororwa ne sub-cellular resolution (5 µm).

s1000 (1)

2) Multi-level resolution analysis: Flexible multi-level analysis kubva ku100 μm kusvika ku5 μm kugadzirisa zvakasiyana-siyana zvenyama pakugadzirisa kwakakwana.

s1000 (2)

3) Comprehensive transcriptome profiling: Zvinyorwa zvakatorwa kubva kune yese tissue slide zvinogona kuongororwa, pasina kudziviswa pahuwandu hwechinangwa chemajini uye nzvimbo inotarirwa.

s1000 (3)

Sevhisi Zvinotsanangurwa

Library

Sequencing strategy

Data Output inokurudzirwa

S1000 cDNA raibhurari

BMKMANU S1000-Illumina PE150

60Gb / muenzaniso

Sample Zvinodiwa

Muenzaniso

Number

Size

RNA Hunhu

OCT yakamisikidzwa tishu block

2-3 zvidhinha / muenzaniso

Approx.6.8x6.8x6.8 mm3

RIN≥7

Kuti uwane rumwe ruzivo nezve nhungamiro yegadziriro yemuenzaniso uye mafambiro ebasa, ndapota inzwa wakasununguka kutaura kune a

Kuyerera Kwebasa Rebasa

Muenzaniso QC

Kuedza kugadzira

sampuli yekuendesa

Muenzaniso wekutumira

Pilot experiment

Pre-kuedza

Spatial barcoding

Spatial barcoding

Kugadzirira Raibhurari

Kuvakwa kweraibhurari

Sequencing

Sequencing

Data analysis

Data analysis

Mushure mekutengesa Services

Mushure mekutengesa masevhisi


  • Zvakapfuura:
  • Zvinotevera:

  • 10x (9)

    Iyo data yakagadzirwa neBMKMANU S1000 inoongororwa uchishandisa software "BSTMatrix", iyo yakazvimiririra yakagadzirwa neBMKGENE, kusanganisira:

    1) Gene kutaura matrix chizvarwa
    2) HE mufananidzo kugadzirisa
    3) Inoenderana neyakadzika yechitatu-bato software yekuongorora
    4) Pamhepo "BSTViewer" inobatsira kuwana mhedzisiro yekuona pane zvakasiyana zvigadziriso.

    1.Spot clustering

    s1000 (4)

    2.Spatial distribution

    s1000 (5)s1000 (6)

    Note: Chisarudzolevel=13 (100 µm, ruboshwe); 7 (50 µm, rudyi)

    3.Marker kutaura kuwanda kuunganidza heatmap

    s1000 (7)

    4.Inter-sample data analysis

    s1000 (8)

     

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