Illumina uye BGI
Features
●Mapuratifomu:Illumina NovaSeq 6000, NovaSeq , HiSeq X Ten uye BGI-DNB-T7
●Sequencing modes:PE50, PE100, PE150, PE250
●Kudzora kunaka kwemaraibhurari kusati kwateedzana
●Kuteedzera kuburitsa data uye QC:kuendeswa kweQC mushumo uye mbishi data mune fastq fomati mushure me demultiplexing uye kusefa Q30 inoverengwa.
Basa Rinobatsira
●Kusiyana-siyana kweSequencing masevhisi:mutengi anogona kusarudza kutevedzana nemugwagwa, kuyerera sero kana huwandu hwe data.
●Kusiyana-siyana kwemapuratifomu:DNB maraibhurari anogona kuendeswa kune Illumina mapuratifomu
●Zvakawanda ruzivo paIllumina sequencing chikuva:nezviuru zvemapurojekiti akavharwa ane marudzi akasiyana-siyana.
●Kuendeswa kwekuteedzera QC mushumo:nemhando metrics, kurongeka kwedata uye kuita kwese kweprojekiti yekuteedzera.
●Mature sequencing process:nenguva pfupi yekutenderera.
●Rigorous Quality Control: Isu tinoshandisa zvakaomesesa QC zvinodiwa kuti tive nechokwadi chekuunza zvinogara zvemhando yepamusoro mhedzisiro.
Sample Zvinodiwa*
Partial Lane Sequencing
| Nhamba yedata (X) | Concentration (qPCR/nM) | Volume |
| X ≤ 50 Gb | ≥ 2 nM | ≥ 20 μl |
| 50 Gb ≤ X <100 Gb | ≥ 3 nM | ≥ 20 μl |
| X ≥ 100 Gb | ≥ 4 nM | ≥ 20 μl |
Single Lane (Illumina)
| Platform | Concentration (qPCR/nM) | Volume |
| HiSeq X Gumi | ≥ 2 nM | ≥ 20 μl |
| NovaSeq 6000 SP | ≥ 1 nM | ≥ 25 μl |
| NovaSeq 6000 S4 | ≥ 1.5 nM | ≥ 25 μl |
| NovaSeq X | ≥ 1.5 nM | ≥ 25 μl |
| BGI-DNBSEQ-T7 | ≥ 1.5 nM | ≥ 25 μl |
Pamusoro pekutarisa uye huwandu hwakazara, yakakodzera peak pateni inodiwa zvakare.
Ndokumbira utaure nesu kana masampula ako akasasangana nekutanga zvinhu zvinodiwa.
Service Workflow
Raibhurari yemhando yekudzora
Sequencing
Data quality control
Kuendesa kweprojekiti
Raibhurari QC mushumo
Chirevo chemhando yeraibhurari inopihwa isati yateedzana, kuongorora huwandu hweraibhurari, uye kupatsanurwa.
Sequencing QC report
Tafura 1. Nhamba dzekuenzanisa data.
| Muenzaniso ID | BMKID | Raw inoverenga | Raw Data (bp) | Kuverenga kwakachena (%) | Q20(%) | Q30(%) | GC(%) |
| C_01 | BMK_01 | 22,870,120 | 6,861,036,000 | 96.48 | 99.14 | 94.85 | 36.67 |
| C_02 | BMK_02 | 14,717,867 | 4,415,360,100 | 96.00 | 98.95 | 93.89 | 37.08 |
Mufananidzo 1. Kugovera kwehutano pamwe chete nekuverenga mumuenzaniso wega wega
Mufananidzo 2. Base content distribution
Mufananidzo 3. Kugovera zvinyorwa zvekuverenga mukuenzanisa data
