DNA / RNA Sequencing - Nanopore Sequencer

ONT sequencing ndeye imwe chete molecule chaiyo-nguva yemagetsi chiratidzo chekuenzanisa tekinoroji yakavakirwa nanopores, iyo yekuteedzera musimboti wepuratifomu imwe neimwe yakafanana.DNA/RNA yakasungwa kaviri inosungirira kune nanoporous protein yakanyudzwa mubiofilm uye ichisununguka pasi pekutungamira kwemota protein, pasi pekuita kwevoltage musiyano kubva kumativi ese ebiofilm, DNA/RNA tambo dzinopfuura nemu nanopore chiteshi protein pane imwe nzvimbo. rate.Nekuda kwekusiyana kwemakemikari ezvigadziko zvakasiyana paDNA / RNA strand, kana chigadziko chimwe chete kana DNA molecule ichipfuura nemu nanopore chiteshi, zvinokonzeresa shanduko yemagetsi akasiyana masaini.Nekuona uye kuenderana nezviratidzo izvi, mhando dzepasi dzinoenderana dzinogona kuverengerwa, uye chaiyo-nguva yekuona yekutevedzana inogona kupedzwa.

Taura nesu

Service Details

Demo Result

Service Details Features

Platform	Library Size	Theoretical Data Kuburitsa (Per Cell)	Single-base Kururama	Applications
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Sero	85-92%	SV kufona, De novo, Yakazara-kureba kutevedzana, Iso-Seq, Gene annotation, Kuonekwa kweDNA methylation

Basa Rinobatsira

● Kupfuura makore mashanu 'ruzivo paPacBio sequencing chikuva nezviuru zvemapurojekiti akavharwa ane marudzi akasiyana.
● BMKGENE ishamwari yepamutemo yeOxford Nanopore, ine dual platform RNA/DNA certification.
● Kune mamodheru makuru evatevedzeri vane midziyo yakakwana uye yakakwana sequencing throughput.
● Kubva papuratifomu yeNanopore, zvinopfuura 10 mhuka nemiti Denovo tsvakurudzo dzakabudiswa mumagazini ane mukurumbira pasi rose.

Muenzaniso Zvinodiwa

Muenzaniso Type

Mari

Concentrtion(Qubit ®)

Volume

Kuchena

Vamwe

Genomic DNA

Zvichienderana neData Inodiwa

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5;

Yakajeka peak pa260 nm, hapana kusvibiswa

Concentration inoda kuyerwa neQubit uye Qubit/Nanopore ≤ 2

Zvose RNA

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5;hapana kusvibiswa

RIN kukosha ≥7.5

Service Workflow

Kugadzirira kwemuenzaniso

Kuvakwa kweraibhurari

Sequencing

Data analysis

Kuendesa kweprojekiti

Zvakapfuura: DNA / RNA Sequencing - Illumina Sequencer

Zvinotevera: Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Data Quality Kuongororwa kweDNA Sample

Tafura 1. Nhamba dze data yakachena.

BMKID	rawSeqNum	rawSumBase	cleanSeqNum	cleanSumBase	cleanN50Len	cleanN90Len	cleanMeanLen	cleanMaxLen	cleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9