BMKCloud Pinda mukati

Zvigadzirwa

Kutevedzana kweDNA/RNA - Nanopore Sequencer

ONT sequencing imorekuru imwe chete inotevedzana nemagetsi panguva chaiyo yakavakirwa pa nanopores, musimboti wekutevedzana kwepuratifomu yega yega wakafanana. DNA/RNA ine tambo mbiri inosungana neprotein ine mapapiro maviri iri mubiofilm uye ichisunungura pasi pekutungamirirwa kweprotein yemota, pasi pekushanda kwemusiyano wevoltage kubva kumativi ese ebiofilm, tambo dzeDNA/RNA dzinopfuura nepakati peprotein yenanopore padanho rakati. Nekuda kwekusiyana kwehunhu hwemakemikari emabhesi akasiyana paDNA/RNA strand, kana base imwe chete kana DNA molecule inopfuura nepakati penanopore chiteshi, inokonzera shanduko yezviratidzo zvemagetsi zvakasiyana. Nekuona uye kuenderana nezviratidzo izvi, mhando dzebase dzinoenderana dzinogona kuverengerwa, uye kuona kwesequence panguva chaiyo kunogona kupedzwa.

Taura nesu

Ruzivo rweBasa

Mhedzisiro yeDemo

Ruzivo rweBasa Zvimiro

Puratifomu	Saizi yeRaibhurari	Kuburitswa kweData redzidziso (Pasero)	Kururama kwechinhu chimwe chete	Mashandisirwo
Nanopore	8Kb, 10kb, 20kb, Ultralong, cDNA-PCR	70-90Gb/Sero	85-92%	Kufona kweSV, De novo, Kutevedzana kwakazara, Iso-Seq, Kutsanangurwa kweGene, Kuonekwa kweDNA methylation

Zvakanakira Basa

● Anopfuura makore mashanu eruzivo paPacBio sequencing platform nezviuru zvemapurojekiti akavharwa ane marudzi akasiyana-siyana.
● BMKGENE ishamwari yepamutemo yeOxford Nanopore, ine chitupa cheRNA/DNA chepuratifomu mbiri.
● Kune mhando dzema sequencers ane michina yakakwana uye throughput yakakwana yeku sequencing.
● Zvichibva papuratifomu yeNanopore, tsvakurudzo dzinopfuura gumi dzeDenovo dzemhuka nemiti dzakaburitswa mumamagazini anozivikanwa pasi rose.

Zvinodiwa zveMienzaniso

Rudzi rwemuenzaniso

Huwandu

Kuisa pfungwa (Qubit ®)

Vhoriyamu

Kuchena

Zvimwe

DNA yeGenomic

Zvichienderana nezvinodiwa neData

≥20ng/μl

≥15μl

OD260/280=1.7-2.2;

OD260/230≥1.5；

Rakapenya zvakanyanya pa 260 nm, hapana kusvibiswa

Kuwanda kwesimba kunofanirwa kuyerwa neQubit neQubit/Nanopore ≤ 2

Huwandu hweRNA

≥1.2μg

≥100μg/μl

≥15μl

OD260/280=1.7-2.5;

OD260/230=0.5-2.5；hapana kusvibiswa

Kukosha kweRIN ≥7.5

Mafambiro Ebasa

Kugadzirira muenzaniso

Kuvakwa kweraibhurari

Kutevedzana kwezvikamu

Kuongorora data

Kuendeswa kwepurojekiti

Yakapfuura: Maraibhurari akagadzirwa kare eIllumina

Zvinotevera: Kutevedzana kweZvikamu Zvakatsanangurwa zveLocus (SLAF-Seq)

Kuongororwa Kwemhando Ye data reDNA Sampuli

Tafura 1. Nhamba dzedata rakachena.

BMKID	rawSeqNum	rawSumBase	cleanSeqNum	cleanSumBase	cleanN50Len	cleanN90Len	cleanMeanLen	cleanMaxLen	cleanMeanQual
DNA_BMK01	1,218,239	26.37	1,121,736	25.90	28,014	15,764	23,090	143,181	9